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Micropropagation and production of arbutin in oriental strawberry tree (Arbutus andrachne L.)

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Abstract

A protocol for micropropagation of Arbutus andrachne from seeds was developed. Results indicated that none of the seeds cultured on Murashige and Skoog (MS) medium, with or without plant growth regulators (PGRs), germinated. Seeds soaked in 250 mg l−1 gibberellic acid (GA3) at 4°C for 3 days, then cultured on water-agar medium containing 2.0 mg l−1 GA3 exhibited 80–100% germination and developed into usable seedlings. Shoot proliferation was tested on MS or B5 medium containing different concentrations of cytokinin. No shoot proliferation was observed on PGR-free medium. Proliferation was more successful on MS than on B5 medium. On both media, the most successful proliferation was obtained using zeatin as a cytokinin type. Rooting was tested on MS medium containing different concentrations of auxin. Rooting failed on PGR-free medium and on medium containing indole-3-acetic acid (IAA), 0.25 or 0.5 mg l−1 indole-3-butyric acid (IBA), or 0.25, 0.5 or 2.0 mg l−1 α-naphthaleneacetic acid (NAA). Rooting (40%) was most successful with 1.0 mg l−1 NAA. Rooted plantlets exhibited 80% survival in all mixtures of peatmoss and perlite, and acclimatized plants were successfully grown in the greenhouse. Quantitative analysis of arbutin performed on in vivo and in vitro leaves using high-performance liquid chromatography (HPLC) revealed that in vivo leaves contained higher arbutin content (0.3–0.81% w/w) than in vitro leaves (0.09% w/w). The highest yield of arbutin in vivo was detected in leaves collected in August, and the lowest yield in leaves collected in December.

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Abbreviations

BA:

6-Benzyladenine

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

NAA:

α-Naphthaleneacetic acid

PGRs:

Plant growth regulators

PVP:

Polyvinylpyrrolidone

TDZ:

1-Phenyl-3-(1,2,3-thiadiazo-5-yl)urea

2iP:

6-(γ,γ,Dimethylallylamino)-purine

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Acknowledgments

The authors would like to thank the Deanship for Scientific Research at Jordan University of Science and Technology for funding this study (Project #187/2007).

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Authors and Affiliations

  1. The National Center for Agricultural Research and Extension, Ramtha, Jordan

    Soha E. Mostafa

  2. Plant Production Department, Faculty of Agriculture, Jordan University of Science and Technology, P.O. Box 3030, Irbid, 22110, Jordan

    Nabila S. Karam

  3. Department of Horticulture and Agronomy, College of Agriculture, University of Jordan, Amman, Jordan

    Rida A. Shibli

  4. Medicinal Chemistry and Pharmacognosy Department, Faculty of Pharmacy, Jordan University of Science and Technology, P.O. Box 3030, Irbid, 22110, Jordan

    Feras Q. Alali

Authors
  1. Soha E. Mostafa
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  2. Nabila S. Karam
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  3. Rida A. Shibli
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  4. Feras Q. Alali
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Correspondence to Nabila S. Karam.

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Mostafa, S.E., Karam, N.S., Shibli, R.A. et al. Micropropagation and production of arbutin in oriental strawberry tree (Arbutus andrachne L.). Plant Cell Tiss Organ Cult 103, 111–121 (2010). https://doi.org/10.1007/s11240-010-9760-2

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  • DOI: https://doi.org/10.1007/s11240-010-9760-2

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