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Improved growth and quality of Siraitia grosvenorii plantlets using a temporary immersion system

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Abstract

The effects of temporary immersion system (TIS) culture on the growth and quality of Siraitia grosvenorii plantlets were investigated. The TIS promoted the growth and quality of S. grosvenorii plantlets. Proliferation rate, shoot length, fresh weight (FW) and dry weight (DW) of shoots, and total biomass production were significantly (P ≤ 0.05) higher in the TIS than in gelled and liquid medium, respectively. The TIS also decreased callus formation at the base of shoots. Callus diameter was significantly (P ≤ 0.05) lower in the TIS (3.30 mm) than in gelled medium (6.31 mm) and liquid medium (6.77 mm), respectively. FW (50.83 mg) and DW (7.08 mg) of callus in the TIS were also significantly (P ≤ 0.05) lower than those in gelled medium (80.00 and 10.56 mg, respectively) and liquid medium (218.75 and 23.75 mg, respectively). During rhizogenesis, minimal callus was evident at the base of shoots in the TIS, with a well-developed root system. However, the plantlets in gelled medium just produced thick, brown and easily broken roots with obvious callus and fewer secondary roots. The natural-like plantlets of S. grosvenorii obtained in the TIS would probably have positive effects on ex vitro rooting and transplanting in large-scale commercial production.

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Fig. 1

Abbreviations

BAP:

6-Benzylaminopurine

DW:

Dry weight

ETR:

Electron transfer rate

Fv/Fm:

Maximal photochemical efficiency of PSII

FW:

Fresh weight

MS:

Murashige and Skoog (1962) (medium)

NAA:

Naphthalene acetic acid

PA:

Photoautotrophic micropropagation

PM:

Photomixotrophic micropropagation

TIS:

Temporary immersion system

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Acknowledgments

This research was financially supported by the Innovation Foundation for PhD of Guangxi University (200810593090101D020), and the Research Foundation granted by the Guangxi Academy of Agricultural Sciences (200814 JI), Nanning, Guangxi, China. Comments and suggestions on the manuscript from the editor, the associate editor and three anonymous reviewers are gratefully acknowledged. We are also grateful to Prof. Toyoki Kozai in Japan and Dr. Manoj Kumar Srivastava from India for their kind help in going through and polishing the manuscript.

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Authors and Affiliations

  1. Bio-Technology Research Institute, Guangxi Academy of Agricultural Sciences, 530007, Nanning, Guangxi, People’s Republic of China

    Huabing Yan & Yangrui Li

  2. Agricultural College, Guangxi University, 530007, Nanning, Guangxi, People’s Republic of China

    Chunxiu Liang

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  1. Huabing Yan
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  2. Chunxiu Liang
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Correspondence to Huabing Yan.

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Yan, H., Liang, C. & Li, Y. Improved growth and quality of Siraitia grosvenorii plantlets using a temporary immersion system. Plant Cell Tiss Organ Cult 103, 131–135 (2010). https://doi.org/10.1007/s11240-010-9752-2

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