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Direct adventitious shoot induction and plant regeneration of Embelia ribes Burm F.

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Abstract

An efficient micropropagation system via direct shoot organogenesis from hypocotyl segments of Embelia ribes Burm F. was developed. A high frequency (84%) of adventitious shoot induction was obtained on Murashige and Skoog (MS) medium supplemented with additives (283.85 μM ascorbic acid [AA], 118.96 μM citric acid [CA], 142.33 μM cysteine, and 684.22 μM glutamine) and 1.13 μM of thidiazuron (TDZ) after 4 weeks following culture. Further development of shoot primordia into well-grown shoots of 4–5 cm in length was achieved by sub-culturing explants along with shoot primordia on MS medium supplemented with 0.44 μM benzyl adenine (BA) and 0.49 μM indole butyric acid (IBA) for three sub-culture periods with an interval of 15 days between them. The highest shoot multiplication was obtained when explants were incubated on MS medium supplemented with 2.2 μM BA and 0.49 μM IBA in 4 weeks. All in vitro developed shoots, 3–4 cm in length, rooted when grown on half-strength MS basal medium along with 2.47 μM IBA within 4 weeks. Moreover, 100% of shoots developed roots when these were treated with 4.93 μM IBA for 20 min and then transferred to pots containing soilrite mix and grown in the greenhouse. In vitro and ex vitro rooted plants showed a survival of 85 and 95% respectively, during hardening in the greenhouse for a 6-week period.

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Abbreviations

AA:

Ascorbic acid

ANOVA:

Analysis of variance

BA:

Benzyl adenine

CA:

Citric acid

GA3 :

Gibberellic acid

HF:

Hormone-free

IBA:

Indolebutyric acid

Kn:

6 furfuryl amino purine

NAA:

α-naphthalene acetic acid

NOA:

Naphthoxyacetic acid

MS:

Murashige and Skoog medium

PGR:

Plant growth regulator

TDZ:

Thidiazuron (n-phenyl-n′-1, 2, 3-thidiazol-5-urea)

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Acknowledgments

This study was carried out with the financial support of the Department of Science and Technology, Govt. of India, New Delhi. The authors are thankful to the Director of the Institute of Wood Science and Technology and the Group Coordinator of Research for the encouragement and providing the use of facilities. FRLHT, Bangalore, and the Karnataka State Forest Department are acknowledged for providing the plant material. The authors are grateful to Dr. Prasad Rallabhandi, M.S., Ph.D., Assistant Professor, Department of Microbiology and Immunology, University of Maryland, School of Medicine, 685W. Baltimore Street, Baltimore, MD21201, USA, for critically reading the manuscript and suggesting the necessary corrections.

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  1. Tree Improvement and Propagation Division, Institute of Wood Science and Technology, 18th Cross, Malleswaram, Bangalore, 560 003, India

    D. Annapurna & T. S. Rathore

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  1. D. Annapurna
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  2. T. S. Rathore
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Correspondence to D. Annapurna.

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Annapurna, D., Rathore, T.S. Direct adventitious shoot induction and plant regeneration of Embelia ribes Burm F.. Plant Cell Tiss Organ Cult 101, 269–277 (2010). https://doi.org/10.1007/s11240-010-9684-x

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