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High-frequency direct shoot regeneration from Drymaria cordata Willd. leaves

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Abstract

An efficient and reproducible procedure is described for direct shoot regeneration in Drymaria cordata Willd. using leaf explants cultured on Murashige and Skoog (MS) medium supplemented with α-naphthalene acetic acid (NAA) and 6-benzyladenine. The regeneration frequency varied with the plant growth regulator concentrations, orientation of the explants, and the carbon source and basal salts present in the regeneration medium. The highest mean number of shoots per explant (10.65 ± 1.03) was recorded on MS plates containing 3% sucrose and 0.8% agar supplemented with 0.1 mg/l NAA and 1.0 mg/l BAP. Shoot buds were induced in the basal parts of the leaf explants. Concentrations of NAA exceeding 1 mg/l suppressed shoot regeneration. Explants bearing the entire lamina and petiole were much more responsive than those having only the lamina. The plantlets that regenerated from the leaf explants were rooted successively on MS medium alone or in combination with indole butyric acid (IBA). The highest mean number of root organogenesis, with 25.67 ± 3.68 roots per leaf segment, was obtained in the presence of 1 mg/l IBA. Histological investigations of the regenerating shoots showed that the shoot buds had emerged from epidermal cells without callus formation. More than 90% of the in vitro-propagated plants survived when transferred to a greenhouse for acclimatization. Thus, this optimized regeneration system may be used for rapid shoot proliferation and genetic transformation.

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Abbreviations

PGR:

Plant growth regulator

MS:

Murashige and Skoog

SH:

Schenk and Hildebrand

B5:

Gambrog et al. (1968) medium

IBA:

Indole-3-butyric acid

BA:

6-Benzyladenine

TDZ:

Thidiazuron

NAA:

Naphthalene acetic acid

2,4-D:

Dicholorophenoxy acetic acid

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Acknowledgments

This work was supported by Konkuk University BrainPool for Dr B. K. Ghimire.

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Authors and Affiliations

  1. Department of Applied Life Science, Konkuk University, Seoul, 143-701, South Korea

    B. K. Ghimire, E. H. Kim & I. M. Chung

  2. Bioherb Research Institute, Kangwon National University, Chunchon, 200-701, South Korea

    E. S. Seong, E. J. Goh & C. Y. Yu

  3. Food Srevice Cuisine Songho College, Hoingsung, 225-704, South Korea

    N. Y. Kim

  4. Department of Horticulture, Kangwon National University, Chunchon, 200-701, South Korea

    W. H. Kang

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  1. B. K. Ghimire
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  2. E. S. Seong
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  3. E. J. Goh
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  4. N. Y. Kim
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  5. W. H. Kang
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  6. E. H. Kim
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  8. I. M. Chung
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Correspondence to I. M. Chung.

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Ghimire, B.K., Seong, E.S., Goh, E.J. et al. High-frequency direct shoot regeneration from Drymaria cordata Willd. leaves. Plant Cell Tiss Organ Cult 100, 209–217 (2010). https://doi.org/10.1007/s11240-009-9627-6

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  • DOI: https://doi.org/10.1007/s11240-009-9627-6

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