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In vitro induction of inflorescence in Dioscorea zingiberensis

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Abstract

Inflorescence induction and morphogenesis of regenerated flowers were investigated in vitro in Dioscorea zingiberensis C. H. Wright. Inflorescence induction was influenced by the type and concentration of phytohormones. When floral bud explants were incubated on a Murashige and Skoog medium containing a combination of 2.0 mg l−1 6-benzyladenine and 0.5 mg l−1 indole-3-butyric acid, the highest frequency of inflorescence induction was observed. However, in the presence of gibberellic acid, induction efficiency was reduced although node length of inflorescence was increased. Ontogenetic studies revealed that the inflorescence primordia originated directly from axillary epidermal cells of the perianth and bract of the explants after 7 days. In vitro, male flowers developed normally and blossomed after 90–100 days. In addition, some bisexual flowers were observed. These results demonstrated that there were differences in sexual differentiation of floral buds in vitro compared with that in vivo.

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Abbreviations

BA:

6-Benzyladenine

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

KT:

Kinetin

MS:

Medium Murashige and Skoog medium

NAA:

Naphthaleneacetic acid

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Acknowledgments

We greatly appreciate the help of Dr. Jihong Liu in revising this paper. This work was supported by grants from the Ph.D. Programs Foundation of Ministry of Education of China (No. 2005050416).

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Authors and Affiliations

  1. College of Life Science and Technology, Huazhong Agricultural University, 430070, WuHan, Hubei, China

    Xiao-Long Huang, Bo Yang & Jia-Ling Yao

  2. College of Horticulture and Forestry Science, Huazhong Agricultural University, 430070, WuHan, Hubei, China

    Chun-Gen Hu

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  1. Xiao-Long Huang
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  2. Bo Yang
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  4. Jia-Ling Yao
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Correspondence to Jia-Ling Yao.

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Huang, XL., Yang, B., Hu, CG. et al. In vitro induction of inflorescence in Dioscorea zingiberensis . Plant Cell Tiss Organ Cult 99, 209–215 (2009). https://doi.org/10.1007/s11240-009-9595-x

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  • DOI: https://doi.org/10.1007/s11240-009-9595-x

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