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Factors contributing to in vitro shoot-tip necrosis and their physiological interactions

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Abstract

Plant tissue culture plays an important role in the production and conservation of plant species. Its application, however, is hindered by some growth abnormalities such as shoot-tip necrosis (STN) caused by the culture conditions. This review article summarizes the literature published on the causes of in vitro STN in plants such as medium type, plant growth regulators, calcium, boron, medium additives, the culture environment, their interaction and physiological effects.

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Abbreviations

ABA:

Abscisic acid

B:

Boron

BA:

[6-benzylaminopurine]

Ca:

Calcium

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

iP:

N6-isopentenyladenine

MS medium:

Murashige and Skoog (1962) basal medium

mT:

meta-Topolin

mTR:

meta-Topolin riboside

PGR:

Plant growth regulators

STN:

Shoot-tip necrosis

TDZ:

Thidiazuron

WPM:

Woody plant medium

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Authors and Affiliations

  1. Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa

    Michael W. Bairu, Wendy A. Stirk & Johannes Van Staden

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  1. Michael W. Bairu
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  2. Wendy A. Stirk
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  3. Johannes Van Staden
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Correspondence to Johannes Van Staden.

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Bairu, M.W., Stirk, W.A. & Van Staden, J. Factors contributing to in vitro shoot-tip necrosis and their physiological interactions. Plant Cell Tiss Organ Cult 98, 239–248 (2009). https://doi.org/10.1007/s11240-009-9560-8

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