Skip to main content
SpringerLink
Log in

The effect of boron on calcium uptake and growth in micropropagated potato plantlets

  • Full Papers
  • Published:
Potato Research Aims and scope Submit manuscript

Summary

Boron (B) requirements differ widely among plant species and the concentration range between toxicity and deficiency is less for B than for any other nutrient. Excess B can adversely affect calcium (Ca) uptake and plant growth. Potato cvs Bintje and Norland plantlets were micropropagated on Murashige & Skoog (MS) nutrient medium, supplemented with 3 (MS control level) or 9 mM Ca, and a range of H3BO3 levels (0.025 to 0.300 mM B). Medium B levels of 0.100 and 0.300 mM decreased Ca content in leaves and shoots of cv. Norland, but not Bintje. Medium B level of 0.025 mM, which is 25% of the control MS level, enhanced Ca uptake in cv. Norland and did not compromise normal plantlet growth in either cultivar. This lower H3BO3 level (0.025 mM B), and a B-free gelling agent such as Gelrite, could be used for micropropagation of potato and possibly other species susceptible to Ca defifiency disorders.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abdulnour, J.E., D.J. Donnelly & N.N. Barthakur, 1998a. Enhanced calcium uptake in micropropagated potato plantlets by macroelement dilution.Journal of Plant Nutrition and Soil Science 161:81–85.

    CAS  Google Scholar 

  • Abdulnour, J.E., D.J. Donnelly & N.N. Barthakur, 1998b. Cation and total macroelement concentrations influence Ca uptake in micropropagated potato plantlets.Journal of Plant Nutrition and Soil Science 161:613–617.

    CAS  Google Scholar 

  • Abousalim, A. & S.H. Mentell, 1994. A practical method for alleviating shoot-tip necrosis symptoms inin vitro shoot cultures ofPistachia vera cv. Mateur.Journal of Horticultural Science 69:357–365.

    Google Scholar 

  • Asad, A., R.W. Bell, B. Dell & L. Huang, 1997. External boron requirements for canola (Brassica napus L.) in boron buffered solution culture.Annals of Botany 80:65–73.

    Article  CAS  Google Scholar 

  • Brenchley, W.E. & K. Warington, 1927. The role of boron in the growth of plants.Annals of Botany 41:167–187.

    CAS  Google Scholar 

  • De Fossard, R.A., A. Myint & E.C.M. Lee, 1974. A broad spectrum tissue culture experiment with tobacco (Nicotiana tabacum) pith tissue callus.Physiologia Plantarum 31:125–130.

    Google Scholar 

  • Gaines, T.P. & G.A. Mitchell, 1979. Boron determination in plant tissue by azomethine H method.Communication in Soil Science and Plant Analysis 10:1099–1108.

    CAS  Google Scholar 

  • Ganmore-Neuman, R. & S. Davidov, 1993. Uptake and distribution of calcium in rose plantlets as affected by calcium and boron concentration in culture solution.Plant and Soil 155/156:151–154.

    Google Scholar 

  • Goldberg, S., 1997. Reactions of boron with soils.Plant and Soil 193:35–48.

    Article  CAS  Google Scholar 

  • Gupta, U.C., Y.W. Jame, C.A. Campbell, A.J. Leyshon & W. Nicholaichuk, 1985. Boron toxicity and deficiency: A review.Canadian Journal of Soil Science 65:381–409.

    CAS  Google Scholar 

  • Gupta, U.C. & J.B. Sanderson, 1993. Effect of sulfur, calcium, and boron on tissue nutrient concentration and potato yield.Journal of Plant Nutrition 16:1013–1023.

    CAS  Google Scholar 

  • Hu, H., P.H. Brown & J.M. Labavitch, 1996. Species viability in boron requirement is correlated with cell wall pectin.Journal of Experimental Botany 47:227–232.

    CAS  Google Scholar 

  • Lloyd, G. & B. McCown, 1981. Commercially-feasible micro-propagation of mountain laurel,Kalmia latifolia, by use of shoot tip culture.International Plant Propagator's Society Proceedings 30:421–427.

    Google Scholar 

  • MacKay, D.C., W.M. Langille & E.W. Chipman, 1962. Boron deficiency and toxicity in crops grown on sphagnum peat soil.Canadian Journal of Soil Science 42:302–310.

    CAS  Google Scholar 

  • Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco cultures.Physiologia Plantarum 15:473–497.

    CAS  Google Scholar 

  • Nable, R.O., G.S. Bañuelos & J.G. Paull, 1997. Boron toxicity.Plant and Soil 198: 181–198.

    Google Scholar 

  • Pregno, L.M. & J.D. Armour, 1992. Boron deficiency and toxicity in potato cv. Sebago on an oxisol of the Atherton Tablelands, North Queensland.Australian Journal of Experimental Agriculture 32:251–253.

    Article  CAS  Google Scholar 

  • Roberts, S. & J.K. Rhee, 1990. Boron utilization by potato in nutrient cultures and in field plantings.Communication in Soil Science and Plant Analysis 21:921–932.

    CAS  Google Scholar 

  • Russel, J.A. & B.H. McCown, 1988. Recovery of plants from protoplasts of hybrid-poplar and aspen clones.Plant Cell Reporter 7:59–62.

    Google Scholar 

  • SAS Institute Inc., 1985. SAS for Linear Models: A Guide to the ANOVA and GLM Procedures, SAS Institute, Cary, NC, USA.

    Google Scholar 

  • Scherer, P.A. & E. Muller, 1988. Multielement analysis of agar and Gelrite impurities investigated by inductively coupled plasma emission spectrometry as well as physical properties of tissue culture media prepared with agar or the gelling gum Gelrite.Acta Horticulturae 226:655–658.

    Google Scholar 

  • Sha, L., B.H. McCown & L.A. Peterson, 1985. Occurrence and cause of shoot tip necrosis in shoot cultures.Journal of the American Society for Horticultural Science 110:631–634.

    Google Scholar 

  • Singha, S. & E.C. Townsend, 1985. Mineral nutrient status of crabapple and pear shoots culturedin vitro on varying concentrations of three commercial agars.Journal of the American Society for Horticultural Science 110:407–411.

    CAS  Google Scholar 

  • Song, S.W., H.Y. Cao & W.D. Liu, 1997. Research on the relationship between cotton varieties with different sensitivity to boron-deficiency and calcium absorption.China Cottons 24:9–10.

    Google Scholar 

  • Teasdale, R.D., 1987. Micronutrients. In: J.M. Bonga & D.J. Durzan (Eds), Cell and Tissue Culture in Forestry, Vol. 1. Martinus Nijhoff Publishers, Dordrecht, Netherlands, p. 422.

    Google Scholar 

  • Thomas, R.L., R.W. Sheard & J.R. Moyer, 1967. Comparison of conventional and automated procedures for nitrogen, phosphorus, and potassium analysis of plant material using a single digestion.Agronomy Journal 59:240–243.

    CAS  Google Scholar 

  • Walworth, J.L. & J.E. Muniz, 1993. A compendium of tissue nutrient concentration for field-grown potatoes.American Potato Journal 70:570–597.

    Google Scholar 

  • Williams, R.R., 1991. Factors determining mineral uptakein vitro.Acta Horticulturae 289:165–166.

    Google Scholar 

  • Woodbridge, C.G., J. Wieneke & F. Fuhr, 1974. The interaction between calcium and boron in apple seedlings.Journal of Plant Nutrition and Soil Science 137:177–188.

    CAS  Google Scholar 

  • Yamauchi, T., T. Hara & Y. Sonoda, 1986. Effects of boron deficiency and calcium supply on the calcium metabolism in tomato plants.Plant and Soil 93:223–230.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre de Recherche en Horticulture, Envirotron, Université Laval, G1K 7P4, QC, Canada

    J. E. Abdulnour

  2. Department of Plant Science, McGill University, Macdonald Campus, 21,111 Lakeshore Road, H9X 3V9, Ste Anne de Bellevue, QC, Canada

    D. J. Donnelly

  3. Department of Natural Resource Sciences, Macdonald Campus, McGill University, 21,111 Lakeshore Road, H9X 3V9, Ste Anne de Bellevue, QC, Canada

    N. N. Barthakur

Authors
  1. J. E. Abdulnour
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. J. Donnelly
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. N. Barthakur
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abdulnour, J.E., Donnelly, D.J. & Barthakur, N.N. The effect of boron on calcium uptake and growth in micropropagated potato plantlets. Potato Res 43, 287–295 (2000). https://doi.org/10.1007/BF02358088

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02358088

Additional keywords

Search

Navigation