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Efficient protocol for in vitro production of androgenic haploids of Phlox drummondii

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Abstract

Production of haploid plants has been restricted to only a few ornamental species. In this paper an efficient anther culture protocol has been devised for production of haploid plants of Phlox drummondii, a garden ornamental. Anthers with microspores at early- to late-uninucleate stages were inoculated on MS (Murashige and Skoog, Physiol Plant 15:473–479, 1962) basal medium containing 9% sucrose, 10 μM 2,4-D + 5 μM BA in the dark for callus induction. The callus (~2 mm) was transferred to MS medium containing 3% sucrose + 10 μM BA + 5 μM NAA under a 16 h photoperiod for multiplication. Anther-derived callus showed the greatest shoot differentiation (60% with greater than 3 shoots per culture) at 13 weeks after culture initiation when maintained on MS medium supplemented with 3% sucrose and cytokinin (7.5 μM BA). At 68 weeks, only 4.6% of cultures differentiated with less than one shoot per callus. Anther-derived shoots rooted readily on MS medium containing 7.5 μM IAA. Of 60 plants that regenerated from anther callus, 50% were haploid, 30% diploid, and 20% aneuploid. Developed protocol could be useful for the haploid induction of outcrossing ornamental plants for production of their homozygous double haploids.

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Abbreviations

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

IAA:

Indole-3-acetic acid

Kn:

Kinetin

NAA:

α-Naphthalene acetic acid

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Acknowledgments

We thank the Head, Department of Botany, University of Delhi for providing facilities to conduct this study.

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Author notes

  1. Anupama Razdan

    Present address: School of Life Sciences, Jawaharlal Nehru University, New Delhi, 11006, India

Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110007, India

    Anupama Razdan & Soom Nath Raina

  2. Department of Botany, Ramjas College, University of Delhi, University Enclave, Delhi, 110007, India

    Maharaj Krishen Razdan

  3. Shyam Lal College, University of Delhi, Delhi, India

    Maharaj Krishen Razdan

  4. Department of Genetics, University of Delhi-South Campus, Benito Juarez Road, New Delhi, 110021, India

    Manchikatla Venkat Rajam

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  1. Anupama Razdan
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  2. Maharaj Krishen Razdan
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  3. Manchikatla Venkat Rajam
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  4. Soom Nath Raina
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Correspondence to Maharaj Krishen Razdan.

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Razdan, A., Razdan, M.K., Rajam, M.V. et al. Efficient protocol for in vitro production of androgenic haploids of Phlox drummondii . Plant Cell Tiss Organ Cult 95, 245–250 (2008). https://doi.org/10.1007/s11240-008-9431-8

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  • DOI: https://doi.org/10.1007/s11240-008-9431-8

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