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Efficient callus induction and plant regeneration from anther of Chinese narcissus (Narcissus tazetta L. var. chinensis Roem)

  • Cell Biology and Morphogenesis
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Abstract

Callus culture has, to date, been reported only in a few species of Narcissus. We used anthers of Chinese narcissus (Narcissus tazetta L. var. chinensis Roem) as explants for callus induction and plant regeneration. A high percentage of anthers at the early- to mid-uninucleate microspore stage were responsive on the basal MS medium supplemented with 0.5–1 mg l−1 2,4-dichlorophenoxyacetic acid and 0.5–2 mg l−1 6-benzyladenine under dark conditions. Calli were initiated from anther connective tissue or anther wall tissue, and no division of microspores occurred during callus formation, as determined by histological observation. Using 20 random amplified polymorphic DNA primers, we verified the genetic integrity of the anther-derived plants of Chinese narcissus with respect to the donor plants. These results suggest that anther culture in vitro can provide an efficient new micropropagation technique for Chinese narcissus as well as a new strategy for in vitro mass propagation of other daffodils.

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Abbreviations

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid;

NAA:

α-Naphthaleneacetic acid

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Acknowledgements

We would like to thank Prof. Huiqiao Tian for his helpful suggestions on the experiments. The research was supported by Natural Science Foundation of Fujian province of China (No. F0210009)

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Authors and Affiliations

  1. Department of biology, School of Life Science, Xiamen University, Xiamen, 361005, China

    LinJiao Chen

  2. Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Science, Xiamen University, Xiamen, 361005, China

    LinJiao Chen, XueYi Zhu, Li Gu & Jian Wu

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  1. LinJiao Chen
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  2. XueYi Zhu
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  3. Li Gu
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  4. Jian Wu
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Correspondence to LinJiao Chen.

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Communicated by K. Kamo

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Chen, L., Zhu, X., Gu, L. et al. Efficient callus induction and plant regeneration from anther of Chinese narcissus (Narcissus tazetta L. var. chinensis Roem). Plant Cell Rep 24, 401–407 (2005). https://doi.org/10.1007/s00299-005-0958-4

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