Abstract
Partial desiccation treatment was applied to improve plant regeneration response in irradiated in vitro cultures. Embryogenic callus cultures of sugarcane cv. Co-671 were exposed to different doses of gamma radiation (0–80 Gy) and radiation effect was evaluated in terms of post-irradiation callus recovery, growth and regeneration of plants. Proliferative capacity of cultures was inversely correlated with radiation dose as the percentage surviving cultures or white proliferating clumps (WPC) decreased as the radiation dose increased up to 80 Gy. LD50 was found to be around 20–30 Gy and at higher doses, poor regeneration frequency was observed after 4–6 weeks of post-irradiation culture. To stimulate regeneration response, irradiated cultures were subjected to partial desiccation for 6 h and the treatment resulted in enhanced plant regeneration response. The study suggests that partial desiccation treatment can be useful in stimulating regeneration response of irradiated in vitro cultures.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahloowalia BS (1998) In vitro techniques and mutagenesis for the improvement of vegetatively propagated plants. In: Jain SM, Brar DS, Ahloowalia BS (eds) Somaclonal variation and induced mutations in crop improvement. Kluwer Academic Publishers, Dordrecht, pp 293–309
Charbaji T, Nabulsi I (1999) Effect of low doses of gamma irradiation on in vitro growth of grapevine. Plant Cell Tiss Org Cult 57:129–132
Desai NS, Suprasanna P, Bapat VA (2003) Improvement in somatic embryogenesis, analysis of somaclonal variation and genetic transformation in Indian Sugarcane varieties. In: International Seminar on Sugarcane Genomics and genetic transformation. VSI, Pune, India, pp 29–31
Desai NS, Suprasanna P, Bapat VA (2004) Partial desiccation of embryogenic callus improves plant regeneration frequency in sugarcane (Saccharum spp.). J Plant Biotechnol 6(4):229–233
Etienne H, Montoro P, Ferriere M, Carron MP (1993) Effects of desiccation, medium osmolarity and Abscisic acid on the maturation of Hevea brasiliensis somatic embryos. J Exp Bot 267:1613–1619
Grene R (2002) Oxidative stress and acclimation mechanisms in plants. In: Somerville CR, Myerowitz (eds) The Arabidopsis Book. American Society of Plant Biologists, Rockville, USA, pp 1–20
Guinn G (1976) Water deficit and ethylene evolution by young cotton bolls. Plant Physiol 57:403–405
Hammatt N, Davey MR (1987) Somatic embryogenesis and plant regeneration from cultured zygotic embryos of soybean. J Plant Physiol 128:219–226
Haslekas C, Stacy R, Nygaard V, Culianez-Macia F, Aalen R (1998) The expression of a periredoxin antioxidant gene, AtPer1, in Arabidopsis thaliana is seed specific and related to dormancy. Plant Mol Biol 36:833–845
IRRI (2003) IRRISTAT for Windows. Version 4.4. IRRI, Metro Manila, Philippines
Karmarkar VM, Kulkarni VM, Suprasanna P, Bapat VA, Rao PS (2005) Study of radio-sensitivity to gamma irradiation at different moisture levels in multiple shoot cultures of banana cv. Basrai (AAA). Physiol Mol Biol Plants 11(1):149–152
Kulkarni VM, Ganapathi TR, Suprasanna P, Bapat VA, Rao PS (1997) Effect of gamma irradiation on in vitro multiple shoot cultures of banana (Musa species). J Nucl Agric Biol 26(4):232–240
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Rance IM, Tian W, Mathews H, de Kochko A, Beachy RN, Fauquet C (1994) Partial desiccation of mature embryo derived calli, a simple treatment that dramatically enhances the regeneration ability of indica rice. Plant Cell Rep 12:647–651
Rasheed S-U, Rashid M, Asad S, Zafar Y, Washeed RA (2001) Use of radiation and in vitro techniques for development of salt tolerant mutants in sugarcane and potato. IAEA TECDOC 1227:51–60
Srinivas L, Ganapathi TR, Suprasanna P, Bapat VA (2006) Desiccation and ABA improves conversion of somatic embryos of banana cv. Rasthali (AAB). Indian J Biotechnol 5:521–526
Van Harten AM (1998) Mutation breeding: theory and practical applications. Cambridge Univ. Press, Cambridge
Vertucci CW, Farrant JM (1995) Acquisition and loss of desiccation tolerance. In: Kigal J, Galili G (eds) Seed development and germination. Marcel Dekker, New York, USA, pp 237–271
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Suprasanna, P., Rupali, C., Desai, N.S. et al. Partial desiccation augments plant regeneration from irradiated embryogenic cultures of sugarcane. Plant Cell Tiss Organ Cult 92, 101–105 (2008). https://doi.org/10.1007/s11240-007-9299-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11240-007-9299-z