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PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea

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Abstract

An efficient protocol to improve microspore embryogenesis is established in an important oleiferous crop, Brassica juncea (Indian mustard). Colchicine was used for enhancing microspore embryogenesis and also to obtain doubled haploid embryos. Colchicine at high concentrations (>10 mg l−1), for 24 h, proved convenient for direct recovery of diploid embryos. Higher temperature treatment and an antiauxin PCIB (p-chlorophenoxyisobutyric acid) enhanced microspore embryogenesis significantly as compared to colchicine. An increase in temperature from 32°C to 35°C proved very efficient in increasing embryogenesis by 10-fold. The highest embryogenesis rate was obtained when PCIB was added at 35°C in the culture after 1 day of culture initiation. 20 μM PCIB could enhance microspore embryogenesis by 5-fold. Different abnormal shapes of embryos like lemon, banana, flask and fused cotyledons were observed. Both normal and fused cotyledonous embryos showed normal germination when transferred on the B5 basal medium.

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Abbreviations

DAPI:

4,6-Diamidino-2-phenylindole

PCIB:

p-Chlorophenoxyisobutyric acid

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Acknowledgement

The authors thank European commission for financial assistance.

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Author notes

  1. Pradeep K. Agarwal

    Present address: Marine Algae and Marine Environment, Central Salt and Marine Chemicals Research Institute, Bhavnagar, 364 002, India

  2. S. S. Bhojwani

    Present address: Dayalbagh Educational Institute, Dayalbagh, Agra, 282 005, India

Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110 007, India

    Pradeep K. Agarwal & S. S. Bhojwani

  2. Department of Life Sciences, Bhavnagar University, Bhavnagar, 364 002, India

    Parinita Agarwal

  3. Plant Research International, Wageningen University and Research Centre, 6700 AA , Wageningen, The Netherlands

    Jan B. M. Custers & Chun-ming Liu

Authors
  1. Pradeep K. Agarwal
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  4. Chun-ming Liu
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  5. S. S. Bhojwani
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Correspondence to Pradeep K. Agarwal.

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Agarwal, P.K., Agarwal, P., Custers, J.B.M. et al. PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea . Plant Cell Tiss Organ Cult 86, 201–210 (2006). https://doi.org/10.1007/s11240-006-9108-0

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  • DOI: https://doi.org/10.1007/s11240-006-9108-0

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