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Specific roles of potato glutamine synthetase isoenzymes in callus tissue grown under salinity: molecular and biochemical responses

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Abstract

Soil salinity is an important determinant to crop distribution and productivity. Proline accumulates in response to salt stress. Glutamine synthetase (GS, EC 6.3.1.2) present in the phloem plays a major role in regulating proline content. Potato (Solanum tuberosum L. cv. Désirée) plants appear to have three nuclear GS-encoding genes: one codes for plastidial (GS2) and the other two code for cytosolic (GS1) isoenzymes. Potato plants possess a low to moderate tolerance to salinity. Three callus tissue lines tolerant to increasing levels of salinity were grown under different salt concentrations (0, 50 and 100 mM NaCl) and proline and GS activity levels were determined and transcript and polypeptide accumulation patterns were analysed. The data obtained suggests a differential involvement of each potato GS isoenzyme in the tissue response to salt stress, as a result of a differential GS isogene expression, with one GS1 gene product becoming the main responsible for the GS activity detected in high salt concentrations.

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Acknowledgement

This work was supported partially by the Program PRAXIS XXI, by FCT BD 9663/96.

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Authors and Affiliations

  1. Plant Functional Biology Unit, Institute for Cell and Molecular Biology (IBMC), Botany Department, School of Sciences, University of Porto, 823, 4150-180, Porto, Portugal

    Jorge Teixeira, Susana Pereira, Filipa Queirós & Fernanda Fidalgo

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  1. Jorge Teixeira
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  2. Susana Pereira
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  3. Filipa Queirós
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  4. Fernanda Fidalgo
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Correspondence to Jorge Teixeira.

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Teixeira, J., Pereira, S., Queirós, F. et al. Specific roles of potato glutamine synthetase isoenzymes in callus tissue grown under salinity: molecular and biochemical responses. Plant Cell Tiss Organ Cult 87, 1–7 (2006). https://doi.org/10.1007/s11240-006-9103-5

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  • DOI: https://doi.org/10.1007/s11240-006-9103-5

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