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Production of transgenic Aralia elata regenerated from Agrobacterium rhizogenes-mediated transformed roots

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Abstract

Transgenic hairy roots were induced from petiole and root segments of in vitro plant Aralia elata, a medicinal woody shrub, after co-cultivation with A. rhizogenes ATCC 15834. The percentage of putative hairy root induction from root segments was higher (26.7%) than petiole explants (10.0%). Hairy roots showed active production of lateral roots with vigorous elongation. Transgenic plants were regenerated from hairy roots via somatic embryogenesis. These plants had wrinkled leaves, short petioles and numerous lateral hairy roots. The RT-PCR analysis showed the expression of rol A, B, C, D, aux 1 and 2 genes differed between the transgenic lines. Endogenous IAA level was higher in transgenic than non-transgenic plants. Conclusively, transgenic hairy roots were developed for first time in A. elata and the transgenic hairy root lines showed distinct morphological growth pattern and gene expression.

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Acknowledgements

This research was supported by the Korea Research Foundation (F00007).

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Authors and Affiliations

  1. Division of Forest Resources, College of Forest Sciences, Kangwon National University, 200-701, Chunchon, Republic of Korea

    H.J. Kang, V.R. Anbazhagan, X.L. You, J.S. Yi & Y.E. Choi

  2. Division of Biotechnology, Korea Forest Research Institute (KFRI), 441–350, Suwon, Republic of Korea

    H.K. Moon

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  1. H.J. Kang
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  2. V.R. Anbazhagan
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  3. X.L. You
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  4. H.K. Moon
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  5. J.S. Yi
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  6. Y.E. Choi
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Correspondence to Y.E. Choi.

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Kang, H., Anbazhagan, V., You, X. et al. Production of transgenic Aralia elata regenerated from Agrobacterium rhizogenes-mediated transformed roots. Plant Cell Tiss Organ Cult 85, 187–196 (2006). https://doi.org/10.1007/s11240-005-9070-2

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  • DOI: https://doi.org/10.1007/s11240-005-9070-2

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