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Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome

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Abstract

In the present survey, interspecific somatic protoplast fusion between a dihaploid cultivated potato Solanum L. tuberosum (BF15) and a wild species Solanum verneï (V3) has been performed using the PEG method. Five putative hybrids were first selected. Among them, only two were retained. DNA analysis, using flow cytometry, showed that the first hybrid (VB2) was dihaploid (2n=2x=24 chromosomes), whereas the second (VB1) was hexaploid (2n=6x=72 chromosomes). In the greenhouse, these putative hybrids showed that they were able of raising and producing large tubers but with a modified shape compared to parental ones. Furthermore, they harbored an intermediate leaf morphology compared to their parents. The hybrid nature of these plants was first confirmed according to their esterase and peroxidase isoenzyme patterns. The RAPD analysis of genomic DNA and microsatellite based amplification (I-SSR) showed that both clones VB1 and VB2 are asymmetric somatic hybrids. They seem to have eliminated the major part of V3 parental nucleus but not the totality. The analysis of chloroplast DNA suggests that both hybrid plastomes were the result of a recombination between parental ones.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic

BAP:

6-benzylaminopurine

CPW:

cell and protoplast washing

DAPI:

4,6-diamino-2-phenylindole

IAA:

indole-3-acetic acid

I-SSR:

inter-simple sequence repeat

KM:

Kao and Michayluk basal medium

MS:

Murashige and Skoog basal medium

NAA:

α-naphthalenacetic acid

PEG:

polyethylene glycol

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Authors and Affiliations

  1. Département de Génie Biologique, Laboratoire des Biotechnologies Végétales Appliquées à l’Amélioration des Cultures Ecole Nationale d’Ingénieurs de Sfax, B.P.W 3038, Sfax, Tunisia

    S. Trabelsi & R. Gargouri-Bouzid

  2. Morphogenèse Végétale Expérimentale, Bãt. 360, Université Paris Sud, 91405, Orsay Cedex, France

    F. Vedel & A. Nato

  3. Faculté des Sciences de Tunis, Tunisia

    L. Lakhoua

  4. Département des Sciences et Vie, Faculté des Sciences de Sfax, B.P. 802, 3018, Sfax, Tunisia

    N. Drira

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  1. S. Trabelsi
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  2. R. Gargouri-Bouzid
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  3. F. Vedel
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  4. A. Nato
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  5. L. Lakhoua
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Correspondence to S. Trabelsi.

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Trabelsi, S., Gargouri-Bouzid, R., Vedel, F. et al. Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome. Plant Cell Tiss Organ Cult 83, 1–11 (2005). https://doi.org/10.1007/s11240-005-3667-3

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  • DOI: https://doi.org/10.1007/s11240-005-3667-3

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