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First Proteome Analysis of Poplar-Type Propolis

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Abstract

Propolis is a natural mixture of honeybee-released and plant-derived compounds produced by honeybees. Poplar propolis is rich in bioactive polyphenolic compounds, and due to its many health benefits, it is commonly used as a food supplement or functional food ingredient. However, it is the only honeybee product whose proteome hasn’t been analyzed. Here, we report a first proteome analysis of poplar-type propolis, a challenging glue-type resinous sample for protein characterization. Raw propolis mixture was precipitated with cold acetone to obtain the protein fraction. Proteins were digested with trypsin, and generated peptides were analyzed on nano-ESI-qTOF SYNAPT G2-Si mass spectrometer (MS) by data-independent acquisition (DIA) and data-dependent acquisition (DDA). Identified peptides and inferred proteins suggest the presence of new bioactive molecules as components of propolis. The poplar-type propolis proteome is composed of a mixture of proteins from the Apis and Populus genera. This is the first-ever report of the proteome of any type of propolis.

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Data Availability

All raw data are available upon request to the corresponding author.

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Funding

This study was performed as a part of the project named «Beehive as a natural resource for antibiotic alternatives» (Grant number 2018–74), financed by the private Sweden foundation Ekhagastiftelsen, founded by Gösta Videgård.

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Authors and Affiliations

  1. Apiotix Technologies, Split, Croatia

    Jelena Šuran & Božo Radić

  2. Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

    Jelena Šuran

  3. University of Virginia, Charlottesville, VA, USA

    Dilza Trevisan-Silva

  4. Ruđer Bošković Institute, Proteomics and Mass Spectrometry, Zagreb, Croatia

    Mario Cindrić & Amela Hozić

Authors
  1. Jelena Šuran
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  2. Božo Radić
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  3. Dilza Trevisan-Silva
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  4. Mario Cindrić
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  5. Amela Hozić
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Contributions

All authors contributed to the study conception and design. Raw material collection and preparation were performed by Jelena Šuran and Božo Radić, and material extraction and proteomic analysis was performed by Amela Hozić and Mario Cindrić. Bioinformatic analysis was performed by Dilza Trevisan-Silva. The first draft of the manuscript was written by Dilza Trevisan-Silva and Jelena Šuran, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jelena Šuran.

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Šuran, J., Radić, B., Trevisan-Silva, D. et al. First Proteome Analysis of Poplar-Type Propolis. Plant Foods Hum Nutr 79, 83–89 (2024). https://doi.org/10.1007/s11130-023-01127-w

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