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The L(M196)H mutation in Rhodobacter sphaeroides reaction center results in new electrostatic interactions

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Abstract

New histidine residue was introduced in M196 position in the reaction center of Rhodobacter sphaeroides in order to alter polarity of the BChl dimer’s protein environment and to study how it affects properties and structure of the primary electron donor P. It was shown that in the absorption spectrum of the mutant RC the 6 nm red shift of the Q Y P band was observed together with considerable decrease of its amplitude. The mid-point potential of P/P + in the mutant RC was increased by +65 (±15) mV as compared to the E m P/P + value in the wild-type RC suggesting that the mutation resulted in new pigment–protein interactions. Crystal structure of RC L(M196)H determined at 2.4 Å resolution implies that BChl Р В and introduced histidine-M196 organize new electrostatic contact that may be specified either as π–π staking or as hydrogen–π interaction. Besides, the structure of the mutants RC shows that His-M196 apparently became involved in hydrogen bond network existing in BChl Р В vicinity that may favor stability of the mutant RC.

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Abbreviations

BChl:

Bacteriochlorophyll

BPhe:

Bacteriopheophytin

P :

Primary electron donor

P A and P B :

BChls constituting P

RC:

Reaction center

WT:

Wild type

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Acknowledgments

The authors thank Dr. Maria Khrenova from Moscow State University for helpful discussions and Maria Leonova for technical assistance. This work was supported by “Molecular and Cell Biology” grant and grants from Russian Foundation for Basic Research (13-04-01148a, 12-04-00332, 13-04-40297-H and 4771.2014.4).

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    Tatiana Y. Fufina, Lyudmila G. Vasilieva & Vladimir A. Shuvalov

  2. Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    Azat G. Gabdulkhakov

Authors
  1. Tatiana Y. Fufina
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  2. Lyudmila G. Vasilieva
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  3. Azat G. Gabdulkhakov
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  4. Vladimir A. Shuvalov
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Correspondence to Lyudmila G. Vasilieva.

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Fufina, T.Y., Vasilieva, L.G., Gabdulkhakov, A.G. et al. The L(M196)H mutation in Rhodobacter sphaeroides reaction center results in new electrostatic interactions. Photosynth Res 125, 23–29 (2015). https://doi.org/10.1007/s11120-014-0062-0

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