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Flash-induced consumption of molecular oxygen on the donor side of photosystem II in Mn-depleted subchloroplast membrane fragments: specific effects of manganese and calcium ions

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Abstract

It has been shown that removal of manganese from the water-oxidizing complex (WOC) of photosystem II (PSII) leads to flash-induced oxygen consumption (FIOC) which is activated by low concentration of Mn2+ (Yanykin et al., Biochim Biophys Acta 1797:516–523, 2010). In the present work, we examined the effect of transition and non-transition divalent metal ions on FIOC in Mn-depleted PSII (apo-WOC-PSII) preparations. It was shown that only Mn2+ ions are able to activate FIOC while other transition metal ions (Fe2+, V2+ and Cr2+) capable of electron donation to the apo-WOC-PSII suppressed the photoconsumption of O2. Co2+ ions with a high redox potential (E 0 for Co2+/Co3+ is 1.8 V) showed no effect. Non-transition metal ions Ca2+ by Mg2+ did not stimulate FIOC. However, Ca2+ (in contrast to Mg2+) showed an additional activation effect in the presence of exogenic Mn2+. The Ca2+ effect depended on the concentration of both Mn2+ and Ca2+. The Ca effect was only observed when: (1) the activation of FIOC induced by Mn2+ did not reach its maximum, (2) the concentration of Ca2+ did not exceed 40 μM; at higher concentrations Ca2+ inhibited the Mn2+-activated O2 photoconsumption. Replacement of Ca2+ by Mg2+ led to a suppression of Mn2+-activated O2 photoconsumption; while, addition of Ca2+ resulted in elimination of the Mg2+ inhibitory effect and activation of FIOC. Thus, only Mn2+ and Ca2+ (which are constituents of the WOC) have specific effects of activation of FIOC in apo-WOC-PSII preparations. Possible reactions involving Mn2+ and Ca2+ which could lead to the activation of FIOC in the apo-WOC-PSII are discussed.

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Abbreviations

PSII:

Photosystem II

RC:

Reaction center

WOC:

Water-oxidizing complex

Apo-WOC-PSII:

Photosystem II membrane fragments deprived of the WOC

Cytb 559 :

Cytochrome b 559

Pheo:

Pheophytin—the primary electron acceptor of PSII

P680 :

The primary electron donor of PSII

Q A :

The primary plastoquinone electron acceptor of PSII

Q B :

The secondary plastoquinone electron acceptor of PSII

TyrZ:

Redox active tyrosine residue 161 of D1 protein

DPC:

Diphenylcarbazide

ΔF:

Photoinduced changes of chlorophyll fluorescence yield of PSII

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Acknowledgments

This work was supported by the Russian Foundation of Basic Research (12-04-31166, 12-04-90044, 11-04-00523) and MCB RAS.

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    D. V. Yanykin, A. A. Khorobrykh, S. A. Khorobrykh & V. V. Klimov

  2. Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27, Akademicheskaya St., 220072, Minsk, Belarus

    N. L. Pshybytko

Authors
  1. D. V. Yanykin
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  2. A. A. Khorobrykh
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  3. S. A. Khorobrykh
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  4. N. L. Pshybytko
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  5. V. V. Klimov
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Correspondence to A. A. Khorobrykh.

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Yanykin, D.V., Khorobrykh, A.A., Khorobrykh, S.A. et al. Flash-induced consumption of molecular oxygen on the donor side of photosystem II in Mn-depleted subchloroplast membrane fragments: specific effects of manganese and calcium ions. Photosynth Res 117, 367–374 (2013). https://doi.org/10.1007/s11120-013-9868-4

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