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Involvement of molecular oxygen in the donor-side photoinhibition of Mn-depleted photosystem II membranes

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Abstract

It has been shown by Khorobrykh et al. (Biochemistry (Moscow) 67:683–688, 2002); Yanykin et al. (Biochim Biophys Acta 1797:516–523, 2010); Khorobrykh et al. (Biochemistry 50:10658–10665, 2011) that Mn-depleted photosystem II (PSII) membrane fragments are characterized by an enhanced oxygen photoconsumption on the donor side of PSII which is accompanied with hydroperoxide formation and it was suggested that the events are related to the oxidative photoinhibition of PSII. Experimental confirmation of this suggestion is presented in this work. The degree of photoinhibition was determined by the loss of the capability of exogenous electron donors (Mn2+ or sodium ascorbate) to the reactivation of electron transport [measured by the light-induced changes of chlorophyll fluorescence yield (∆F)] in Mn-depleted PSII membranes. The transition from anaerobic conditions to aerobic ones significantly activated photoinhibition of Mn-depleted PSII membranes both in the absence and in the presence of exogenous electron acceptor, ferricyanide. The photoinhibition of Mn-depleted PSII membranes was suppressed upon the addition of exogenous electron donors (Mn2+, diphenylcarbazide, and ferrocyanide). The addition of superoxide dismutase did not affect the photoinhibition of Mn-depleted PSII membranes. It is concluded that the interaction of molecular oxygen (rather than superoxide anion radical formed on the acceptor side of PSII) with the oxidized components of the donor side of PSII reflects the involvement of O2 in the donor-side photoinhibition of Mn-depleted PSII membranes.

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Abbreviations

PSII:

Photosystem II

RC:

Reaction center

WOC:

Water-oxidizing complex

Mn-depleted PSII membranes:

Photosystem II membrane fragments deprived of manganese

Pheo:

Pheophytin—the primary electron acceptor of PSII

P680 :

The primary electron donor of PSII

QA :

The primary plastoquinone electron acceptor of PSII

QB :

The secondary plastoquinone electron acceptor of PSII

TyrZ:

Redox active tyrosine residue 161 of D1 protein

SOD:

Superoxide dismutase

Cyt c :

Cytochrome c

DPC:

Diphenylcarbazide

ΔF:

Photoinduced changes of chlorophyll fluorescence yield of PSII

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Acknowledgments

This work was supported by the Russian Foundation of Basic Research (14-04-01667 and 14-04-00974) and MCB RAS.

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    A. A. Khorobrykh & V. V. Klimov

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  1. A. A. Khorobrykh
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  2. V. V. Klimov
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Correspondence to A. A. Khorobrykh.

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Khorobrykh, A.A., Klimov, V.V. Involvement of molecular oxygen in the donor-side photoinhibition of Mn-depleted photosystem II membranes. Photosynth Res 126, 417–425 (2015). https://doi.org/10.1007/s11120-015-0135-8

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