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Study of the effect of reducing conditions on the initial chlorophyll fluorescence rise in the green microalgae Chlamydomonas reinhardtii

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Abstract

Incubation of Chlamydomonas reinhardtii cells under nutrient deficiency results in the faster initial rise in the light-induced chlorophyll fluorescence kinetic curve. We showed that short-term anaerobic incubation of algal cells altered initial fluorescence in a way similar to nutrient starvation, suggesting an important role of the plastoquinones redox state in the observed effect. Bi-component analysis of highly resolved initial fluorescence rise kinetics in sulfur- or oxygen-depleted C. reinhardtii cells suggested that one of the mechanisms underlying the observed phenomenon involves primary closure (photochemical inactivation via Qa reduction) of β-type PSII as compared to α-PSII. Moreover, results of modeling of the fluorescence curve brought us to the conclusion that accumulation of closed centers in α-PSII supercomplexes may also cause a faster initial fluorescence rise. The observed correlations between nutrient supply rate and initial fluorescence rise pattern in green algae can serve to characterize culture nutritional status in vivo.

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Abbreviations

Chl:

Chlorophyll

PSII:

Photosystem II

PSU:

Photosynthetic unit

LHCII:

Light-harvesting complex II

PQ:

Plastoquinon

Pheo:

Pheophytin

OEC:

Oxygen-evolving complex

PEE :

Energy exchange probability

PPFD:

Photosynthetic photon flux density

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Acknowledgments

This study was supported by the Russian Foundation for Basic Research (10-04-00864-a) and Russian Federal Target Programme (8077).

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Authors and Affiliations

  1. Faculty of Biology, Moscow State University, Vorobyevi Gory, 119992, Moscow, Russia

    T. K. Antal, A. Kolacheva, A. Maslakov, G. Yu. Riznichenko, T. E. Krendeleva & A. B. Rubin

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  1. T. K. Antal
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  2. A. Kolacheva
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  3. A. Maslakov
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  4. G. Yu. Riznichenko
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  5. T. E. Krendeleva
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  6. A. B. Rubin
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Correspondence to T. K. Antal.

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Antal, T.K., Kolacheva, A., Maslakov, A. et al. Study of the effect of reducing conditions on the initial chlorophyll fluorescence rise in the green microalgae Chlamydomonas reinhardtii . Photosynth Res 114, 143–154 (2013). https://doi.org/10.1007/s11120-012-9789-7

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  • DOI: https://doi.org/10.1007/s11120-012-9789-7

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