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Reduction of the primary donor P700 of photosystem I during steady-state photosynthesis under low light in Arabidopsis

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Abstract

During steady-state photosynthesis in low-light, 830-nm absorption (A830) by leaves was close to that in darkness in Arabidopsis, indicating that the primary donor P700 in the reaction center of photosystem I (PSI) was in reduced form. However, P700 was not fully oxidized by a saturating light pulse, suggesting the presence of a population of PSI centers with reduced P700 that remains thermodynamically stable during the application of the saturating light pulse (i.e., reduced-inactive P700). To substantiate this, the effects of methyl viologen (MV) and far-red light on P700 oxidation by the saturating light pulse were analyzed, and the cumulative effects of repetitive application of the saturating light pulse on photosynthesis were analyzed using a mutant crr2-2 with impaired PSI cyclic electron flow. We concluded that the reduced-inactive P700 in low-light as revealed by saturating light pulse indicates limitations of electron flow at the PSI acceptor side.

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Abbreviations

A830 :

Leaf absorption at 830 nm

Chl:

Chlorophyll

Fm and Fm′:

Maximum fluorescence level in the dark- and light-adapted leaf, respectively

Fs and Fo :

Steady state- and minimum-fluorescence level, respectively

FR:

Far-red light

MV:

Methyl viologen

P700:

Primary donor pigment in the reaction center of PSI

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Acknowledgments

We thank Dr. Toshiharu Shikanai (Kyoto University) for helpful discussions and suggestions. This work was supported by a Grant-in-aid for Young Scientists B (18780117) from MEXT to Michito Tsuyama, for Young Scientists from Kyushu University to Michito Tsuyama, and for Scientific Research on Priority Areas (16085206) from MEXT to Toshiharu Shikanai.

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  1. Department of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Michito Tsuyama & Yoshichika Kobayashi

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  1. Michito Tsuyama
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  2. Yoshichika Kobayashi
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Correspondence to Michito Tsuyama.

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Tsuyama, M., Kobayashi, Y. Reduction of the primary donor P700 of photosystem I during steady-state photosynthesis under low light in Arabidopsis . Photosynth Res 99, 37–47 (2009). https://doi.org/10.1007/s11120-008-9379-x

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