Abstract
MicroRNAs (miRNAs) are small, single-stranded, non-coding endogenous RNAs that play vital roles in the regulation of gene expression at the post-transcriptional level. Several studies of miRNAs in diploid and tetraploid Paulownia plants have been reported; however, miRNAs involved in response to drought stress have not yet been investigated. In this study, six small RNA libraries of Paulownia fortunei, two drought-treated and four controls, were built and then sequenced on an Illumina/Solexa GAIIx platform. A total of 30 conserved miRNAs and 88 novel miRNAs were identified in the sRNA libraries. Among them, 22 miRNAs in common were differentially expressed both in diploid and tetraploid P. fortunei under the drought stress. Degradome sequencing was used to identify the target genes of the miRNAs. Moreover, nine differentially expressed miRNAs and ten target genes were validated by quantitative real-time polymerase chain reaction (qRT-PCR). This study has provided data for further research into the molecular mechanisms associated with drought stress in diploid and autotetraploid P. fortunei plants.
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Acknowledgements
This work was supported by the Joint Funds of the National Natural Science Foundation of China (NSFC) (U1204309), the Fund of the Transformation Project of the National Agricultural Scientific and Technological Achievement of China (2012GB2D000271), the Central Financial Forestry Science Promotion Project (GTH [2012]01), the Fund of the Science Key Program of Department of Henan Education (12A220003), the Fund of the Technology Innovation Team Project of Zhengzhou (121PCXTD515), the Fund of the Science and Technology Key Project of Henan Education Department (14A220001), and the Fund of Zhongyuan Scholarship Foundation of Henan Province (122101110700).
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The raw sequence data are available at the NIH Short Read Archive database (http://www.ncbi.nlm.nih. gov/sra) under the accession number SRP062453 (alias: PRJNA293002).
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Fan, G., Niu, S., Li, X. et al. Functional Analysis of Differentially Expressed MicroRNAs Associated with Drought Stress in Diploid and Tetraploid Paulownia fortunei . Plant Mol Biol Rep 35, 389–398 (2017). https://doi.org/10.1007/s11105-017-1031-5
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DOI: https://doi.org/10.1007/s11105-017-1031-5