Skip to main content
SpringerLink
Log in

A Novel PhLRR Gene Promoter is Sufficient for Engineering Male Sterility in Petunia

  • Original Paper
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

Tissue-specific promoters can drive genes specifically expressed in the target organs and have been widely used in plant molecular breeding. In this study, a 1.2-kb promoter region of an anther-specific gene PhLRR from Petunia hybrida “Fantasy” was isolated and fused to the β-glucuronidase (GUS) gene. The pPhLRR::GUS vector was heterogeneously transformed into tobacco in which the GUS staining was only detected in the early development stage of anthers and no GUS expression in any other three floral whirls or vegetative organs was observed. It is very different from other well-studied anther-specific promoters which drive genes specifically expressed in the later development stage of anthers or only in the pollens. Furthermore, the pPhLRR::Barnase was introduced into petunia and induced complete male sterility without influencing the ornamental characteristics or the female fertility in transformed plants. These results indicate that PhLRR promoter is a new kind of petunia anther-specific promoter and could be taken as a valuable tool in ornamental plant breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ai Y, He Y, Hu Y, Zhang Q, Pan C, Bao M (2014) Characterization of a novel male sterile mutant of Tagetes patula induced by heat shock. Euphytica 200:159–173

    Article  Google Scholar 

  • Bate N, Twell D (1998) Functional architecture of a late pollen promoter: pollen-specific transcription is developmentally regulated by multiple stage-specific and co-dependent activator elements. Plant Mol Biol 37:859–869

    Article  CAS  PubMed  Google Scholar 

  • Chen L, Tu Z, Hussain J, Cong L, Yan Y, Jin L, Yang G, He G (2010) Isolation and heterologous transformation analysis of a pollen-specific promoter from wheat (Triticum aestivum L.). Mol Biol Rep 37:737–744

    Article  PubMed  Google Scholar 

  • Chen L, Miao Y, Wang C, Su P, Li T, Wang R, Hao X, Yang G, He G, Gao C (2012) Characterization of a novel pollen-specific promoter from wheat (Triticum aestivum L.). Plant Mol sBiol Rep 30:1426–1432

    Article  CAS  Google Scholar 

  • Cnudde F, Moretti C, Porceddu A, Pezzotti M, Gerats T (2003) Transcript profiling on developing Petunia hybrida floral organs. Sex Plant Reprod 16:77–85

    Article  CAS  Google Scholar 

  • Cook M, Thilmony R (2012) The OsGEX2 gene promoter confers sperm cell expression in transgenic rice. Plant Mol Biol Rep 30:1138–1148

    Article  CAS  Google Scholar 

  • De Block M, Debrouwer D, Moens T (1997) The development of a nuclear male sterility system in wheat. Expression of the barnase gene under the control of tapetum specific promoters. Theor Appl Genet 95:125–131

    Article  Google Scholar 

  • Drews GN, Goldberg RB (1989) Genetic control of flower development. Trends Genet 5:256–261

    Article  CAS  PubMed  Google Scholar 

  • Endo M, Tsuchiya T, Satio H, Matsubara H, Hakozaki H, Masuko H, Kamada M, Higashitani A, Takahashi H, Fukuda H, Demura T, Watanabe M (2004) Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray. Genes Genet Syst 79:213–226

    Article  CAS  PubMed  Google Scholar 

  • Eyal Y, Curie C, McCormick S (1995) Pollen specificity elements reside in 30 bp of the proximal promoters of two pollen-expressed genes. Plant Cell 7:373–384

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gardner N, Felsheim R, Smith AG (2009) Production of male-and female-sterile plants through reproductive tissue ablation. J Plant Physiol 166:871–881

    Article  CAS  PubMed  Google Scholar 

  • Golkari S, Gilbert J, Prashar S, Procunier JD (2007) Microarray analysis of Fusarium graminearum-induced wheat genes: identification of organ-specific and differentially expressed genes. Plant Biotechnol J 5:38–49

    Article  CAS  PubMed  Google Scholar 

  • Hobo T, Suwabe K, Aya K, Suzuki G, Yano K, Ishimizu T, Fujioka T, Kaneko F, Kazama T, Mizuta Y, Takahashi H, Shiono K, Nakazono M, Tsutsumi N, Nagamura Y, Kurata N, Watanabe M, Matsuoka M (2008) Various spatiotemporal expression profiles of anther-expressed genes in rice. Plant Cell Physiol 49:1417–1428

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Honys D, Twell D (2003) Comparative analysis of the Arabidopsis pollen transcriptome. Plant Physiol 132:640–652

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hsu YF, Tzeng JD, Liu MC, Yei FL, Chung MC, Wang CS (2008) Identification of anther-specific/predominant genes regulated by gibberellin during development of lily anthers. J Plant Physiol 165:553–563

    Article  CAS  PubMed  Google Scholar 

  • Huang X, Yue Y, Sun J, Peng H, Yang Z, Bao M, Hu H (2015) Characterization of a fertility-related SANT/MYB gene (PhRL) from petunia. Sci Hortic 183:152–159

    Article  CAS  Google Scholar 

  • Kato H, Xie G, Sato Y, Imai R (2010) Isolation of anther-specific gene promoters suitable for transgene expression in rice. Plant Mol Biol Rep 28:381–387

    Article  CAS  Google Scholar 

  • Koltunow AM, Truettner J, Cox KH, Wallroth M, Goldberg RB (1990) Different temporal and spatial gene expression patterns occur during anther development. Plant Cell 2:1201–1224

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lännenpää M, Hassinen M, Ranki A, Hölttä-Vuori M, Lemmetyinen J, Keinonen K, Sopanen T (2005) Prevention of flower development in birch and other plants using a BpFULL1: BARNASE construct. Plant Cell Rep 24:69–78

    Article  PubMed  Google Scholar 

  • Lee YH, Chung KH, Kim HU, Jin YM, Kim HI, Park BS (2003) Induction of male sterile cabbage using a tapetum-specific promoter from Brassica campestris L. ssp. pekinensis. Plant Cell Rep 22:268–273

    Article  CAS  PubMed  Google Scholar 

  • Liao L, Liu C, Ning G, Bao M (2012) A pFBP6::Barnase construct resulted in stigma and style ablation and floral abscission in transgenic tobacco. Plant Mol Biol Rep 30:1196–1203

    Article  CAS  Google Scholar 

  • Luo H, Lee J, Hu Q, Nelson-Vasilchik K, Eitas T, Lichwar C, Kausch A, Chandlee J, Hodges T (2006) RTS, a rice anther-specific gene is required for male fertility and its promoter sequence directs tissue-specific gene expression in different plant species. Plant Mol Biol 62:397–408

    Article  CAS  PubMed  Google Scholar 

  • Ma L, Sun N, Liu X, Jiao Y, Zhao H, Deng XW (2005) Organ-specific expression of Arabidopsis genome during development. Plant Physiol 138:80–91

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ma J, Duncan D, Morrow DJ, Fernandes J, Walbot V (2007) Transcriptome profiling of maize anthers using genetic ablation to analyze pre-meiotic and tapetal cell types. Plant J 50:637–648

    Article  CAS  PubMed  Google Scholar 

  • Ma G, Ning G, Zhang W, Zhan J, Lv H, Bao M (2011) Overexpression of Petunia SOC1-like gene FBP21 in tobacco promotes flowering without decreasing flower or fruit quantity. Plant Mol Biol Rep 29:573–581

    Article  CAS  Google Scholar 

  • Mahmood T, Zar T, Saqlan Naqvi S (2008) Multiple pulses improve electroporation efficiency in Agrobacterium tumefaciens. Electron J Biotechnol 11:145–148

    Article  Google Scholar 

  • Mariani C, De Beuckeleer M, Truettner J, Leemans J, Goldberg RB (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347:737–741

    Article  CAS  Google Scholar 

  • Masuko H, Endo M, Saito H, Hakozaki H, Park J, Kawagishi-Kobayashi M, Takada Y, Okabe T, Kamada M, Takahashi H, Higashitani A, Watanabe M (2006) Anther-specific genes, which expressed through microsporogenesis, are temporally and spatially regulated in model legume, Lotus japonicus. Genes Genet Syst 81:57–62

    Article  CAS  PubMed  Google Scholar 

  • Porceddu A, Reale L, Lanfaloni L, Moretti C, Sorbolini S, Tedeschini E, Ferranti F, Pezzotti M (1999) Cloning and expression analysis of a Petunia hybrida flower specific mitotic-like cyclin. FEBS Lett 462:211–215

    Article  CAS  PubMed  Google Scholar 

  • Rogers H, Bate N, Combe J, Sullivan J, Sweetman J, Swan C, Lonsdale D, Twell D (2001) Functional analysis of cis-regulatory elements within the promoter of the tobacco late pollen gene g10. Plant Mol Biol 45:577–585

    Article  CAS  PubMed  Google Scholar 

  • Roque E, Gómez MD, Ellul P, Wallbraun M, Madueño F, Beltrán JP, Cañas LA (2007) The PsEND1 promoter: a novel tool to produce genetically engineered male-sterile plants by early anther ablation. Plant Cell Rep 26:313–325

    Article  CAS  PubMed  Google Scholar 

  • Twell D, Yamaguchi J, McCORMICK S (1990) Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis. Development 109:705–713

    CAS  PubMed  Google Scholar 

  • Uchida W, Matsunaga S, Sugiyama R, Kazama Y, Kawano S (2003) Morphological development of anthers induced by the dimorphic smut fungus Microbotryum violaceum in female flowers of the dioecious plant Silene latifolia. Planta 218:240–248

    Article  CAS  PubMed  Google Scholar 

  • Van Der Meer IM, Stam ME, van Tunen AJ, Mol J, Stuitje AR (1992) Antisense inhibition of flavonoid biosynthesis in petunia anthers results in male sterility. Plant Cell 4:253–262

    Article  PubMed  PubMed Central  Google Scholar 

  • van Tunen AJ, Hartman SA, Mur LA, Mol JN (1989) Regulation of chalcone flavanone isomerase (CHI) gene expression in Petunia hybrida: the use of alternative promoters in corolla, anthers and pollen. Plant Mol Biol 12:539–551

    Article  PubMed  Google Scholar 

  • Van Tunen AJ, Mur LA, Brouns GS, Rienstra JD, Koes RE, Mol J (1990) Pollen-and anther-specific chi promoters from petunia: tandem promoter regulation of the chiA gene. Plant Cell 2:393–401

    Article  PubMed  PubMed Central  Google Scholar 

  • Wei H, Meilan R, Brunner AM, Skinner JS, Ma C, Gandhi HT, Strauss SH (2007) Field trial detects incomplete barstar attenuation of vegetative cytotoxicity in Populus trees containing a poplar LEAFY promoter: barnase sterility transgene. Mol Breed 19:69–85

    Article  CAS  Google Scholar 

  • Willing R, Bashe D, Mascarenhas J (1988) An analysis of the quantity and diversity of messenger RNAs from pollen and shoots of Zea mays. Theor Appl Genet 75:751–753

    Article  CAS  Google Scholar 

  • Yue Y, Ma F, Huang X, Bao M, Liu G, Hu H (2013) Transcriptional profile of differentially expressed genes related to abortive flower buds under short light period stress in petunia. Sci Hortic 164:323–332

    Article  CAS  Google Scholar 

  • Yue Y, Sun J, Huang X, Peng H, Liu G, Hu H (2014) Characterization of a novel anther-specific gene encoding a leucine-rich repeat protein in petunia. Genet Mol Res 13:9889–9898

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This research work was funded by the Fundamental Research Funds for the Central Universities (Project 2013PY085) and the Natural Science Foundation of Hubei Province of China (Project 2014CFB926).

Author information

Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Yuanzheng Yue, Di Yang, Jian Sun, Hao Peng, Chaoqun Yin, Rui Guo, Guogui Ning & Huirong Hu

Authors
  1. Yuanzheng Yue
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Di Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jian Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hao Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chaoqun Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rui Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Guogui Ning
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Huirong Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Huirong Hu.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Fig. S1

(DOC 124 kb)

Fig. S2

(DOC 239 kb)

Table S1

(DOC 35 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yue, Y., Yang, D., Sun, J. et al. A Novel PhLRR Gene Promoter is Sufficient for Engineering Male Sterility in Petunia. Plant Mol Biol Rep 34, 970–977 (2016). https://doi.org/10.1007/s11105-016-0977-z

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11105-016-0977-z

Keywords

Search

Navigation