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The Sophora Alopecuroid H + -PPase Gene SaVP1 Confers Multiple Abiotic Stress Tolerance in Arabidopsis

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Abstract

The physiological role of a vacuolar H+-PPase (SaVP1) from an eremophyte, Sophora alopecuroid, was evaluated through overexpressing in Arabidopsis. Overexpression of SaVP1 in Arabidopsis enhanced tolerance to drought and salt stresses and resulted in the up-regulation of several K+ and Ca2+ channel/ transporters genes, which showed a function similar to that of vacuolar H+-PPase from other plants. Moreover, the transgenic plants showed higher temperature tolerance than wild type plants, and had a higher seed yield. Overexpression of SaVP1 could improve the content of indole-3-acetic acid (IAA) in floral organs under high-temperature stress. Meanwhile, the application of IAA in low concentration led to higher fertility in the SaVP1-overexpressing plants under high temperature. These results indicate that SaVP1 might activate the auxin biosynthesis and signaling pathway in Arabidopsis to improve tolerance to heat. SaVP1 from an eremophyte could also be applied as a candidate gene for enhancing multiple stress tolerance in other plant species.

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Fig. 1a,b
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Abbreviations

SOD:

Superoxide dismutase

POD:

Peroxidases

MDA:

Malondialdehyde

IAA:

Indole-3-acetic acid

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Acknowledgments

The work was funded by the Project from Ministry of Agriculture of China (2013ZX08005-004) and the open funds of the National Key Laboratory of Crop Genetic Improvement (200902).

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Authors and Affiliations

  1. Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alaer, Xinjiang, 843300, China

    Yanqin Wang

  2. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Yanqin Wang, Shuangxia Jin, Ling Min, Xin He, Yaoyao Li, Yi Zhu, Yichun Nie, Xianlong Zhang & Longfu Zhu

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  1. Yanqin Wang
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  2. Shuangxia Jin
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  4. Xin He
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  6. Yi Zhu
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  9. Longfu Zhu
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Correspondence to Longfu Zhu.

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Table S1

Primers used in this research. (XLSX 12 kb)

Figure S1

The sequence of SaVP1 from Sophora alopecuroides. (JPEG 2116 kb)

Figure S2

Relative transcript level of SaVP1 in leaves and roots of Sophora alopecuroides after 0.5 M NaCl treatment for 0h, 4 h, 8h, and 12h, respectively. (JPEG 248 kb)

Figure S3

The biomass of the two transgenic lines and WT plants under the control condition and after drought and salt stress treatments. (JPEG 190 kb)

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Wang, Y., Jin, S., Min, L. et al. The Sophora Alopecuroid H + -PPase Gene SaVP1 Confers Multiple Abiotic Stress Tolerance in Arabidopsis. Plant Mol Biol Rep 33, 923–930 (2015). https://doi.org/10.1007/s11105-014-0801-6

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