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Isolation and Characterization of IaYABBY2 Gene from Incarvillea arguta

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Abstract

The establishment of ad-abaxial polarity is an important characteristic of the development of lateral organs in plants. YABBY genes encode higher plant-specific nuclear proteins which play critical roles in promoting abaxial cell fate. IaYABBY2 (IaYAB2, Genbank accession no. KF250432), isolated from Incarvillea arguta, is a member of YABBY gene family. Sequence characterization and phylogenetic analyses show that IaYABBY2 is a member of the YAB2 subfamily of Arabidopsis thaliana. Subcellular localization analysis indicates that IaYABBY2 is localized in the nucleus. Ectopic expression of IaYABBY2 in Arabidopsis plants resulted in the partial abaxialization of adaxial epidermises of leaves and sepals and development defect of florescence. The transgenic lines also showed higher level of anthocyanin content and photosynthesis capability after differential environment stress. These results indicate that the IaYABBY2 functions in the ad-abaxial polarity, development of shoot apical meristem, and environmental stress.

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Acknowledgments

This article was supported by the Young Academic and Technical Leader Raising Foundation of Yunnan Province (no. 2012HB041).

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Authors and Affiliations

  1. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany Chinese Academy of Sciences, Kunming, 650201, People’s Republic of China

    Xudong Sun, Yanlong Guan & Xiangyang Hu

  2. Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People’s Republic of China

    Xudong Sun, Yanlong Guan & Xiangyang Hu

  3. University of the Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Yanlong Guan

Authors
  1. Xudong Sun
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  2. Yanlong Guan
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  3. Xiangyang Hu
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Correspondence to Xiangyang Hu.

Additional information

Xudong Sun and Yanlong Guan contributed equally to this study.

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Sun, X., Guan, Y. & Hu, X. Isolation and Characterization of IaYABBY2 Gene from Incarvillea arguta . Plant Mol Biol Rep 32, 1219–1227 (2014). https://doi.org/10.1007/s11105-014-0725-1

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  • DOI: https://doi.org/10.1007/s11105-014-0725-1

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