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Cloning and Molecular Characterization of a SERK Gene Transcriptionally Induced During Somatic Embryogenesis in Ananas comosus cv. Shenwan

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Abstract

A somatic embryogenesis receptor-like kinase (SERK) gene, designated as AcSERK1, was isolated from pineapple (Ananas comosus cv. Shenwan). AcSERK1 shared all the characteristic domains of the SERK family, including five leucine-rich repeats, one proline-rich region motif, transmembrane domain, and kinase domains. Somatic embryogenic cultures of pineapple were established following transfer of callus cultures to Murashige and Skoog (1962) medium containing 2,4-dichlorophenoxyacetic acid. The role of AcSERK1 during establishment of somatic embryogenesis in culture was investigated. The AcSERK1 was highly expressed during embryogenic competence acquisition and global embryo formation in culture. These findings were obtained along with morphological changes in callus cultures exhibiting embryogenic potential. Overall, levels of expression of AcSERK1 were lower in nonembryogenic tissues and organs than in embryogenic callus. In situ hybridization analysis revealed that AcSERK1 expression was detected in embryogenic tissues, including single competent cells, meristematic centers wherein embryogenic structures are formed, and global embryos. These results suggested that AcSERK1 expression was associated with induction of somatic embryogenesis and that it could be used as a potential marker gene to monitor the transition of pineapple callus tissues into competent and embryogenic cells and tissues.

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Acknowledgments

This research was supported by the Natural Science Foundation of China (30971984), Project 948 of Ministry of Agriculture (2010-G2-11), Commonweal Industry Scientific Research Project of Ministry of Agriculture (nyhyzx07-30), and Open Found Project of Key Laboratory of Utilization of Tropical Crop Germplasm Resources, Ministry of Agriculture (KFKT-2010-07).

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Authors and Affiliations

  1. Horticultural Biotechnology College of South China Agricultural University, Guangzhou, Guangdong, 510642, China

    Jun Ma, Yehua He, Chenghou Wu, Heping Liu & Zhongyi Hu

  2. College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, 611100, China

    Jun Ma

  3. South Subtropical Crop Research Institute, Zhanjiang, Guangdong, 524091, China

    Guangming Sun

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  1. Jun Ma
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  2. Yehua He
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  3. Chenghou Wu
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  4. Heping Liu
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  5. Zhongyi Hu
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  6. Guangming Sun
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Correspondence to Yehua He.

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Ma, J., He, Y., Wu, C. et al. Cloning and Molecular Characterization of a SERK Gene Transcriptionally Induced During Somatic Embryogenesis in Ananas comosus cv. Shenwan. Plant Mol Biol Rep 30, 195–203 (2012). https://doi.org/10.1007/s11105-011-0330-5

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