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Cloning and Characterization of the BcTuR3 Gene Related to Resistance to Turnip Mosaic Virus (TuMV) from Non-heading Chinese Cabbage

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Abstract

A cDNA clone for the turnip mosaic virus (TuMV)-induced gene in non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino cv. Duanbaigeng) was isolated and characterized. The full-length cDNA clone, designated BcTuR3, was isolated during resistance response to TuMV. Sequence analysis of the cDNA clone confirmed that the translation product of the gene is homologous to other plant resistance proteins. Genomic DNA Southern blot analysis indicated that the gene represented a small multi-gene family. Northern hybridizations confirmed its elevated expression in the resistant “Duanbaigeng” and the susceptible “Aijiaohuang.” Upon inoculation with TuMV, the BcTuR3 transcript was rapidly accumulated in the infected leaves and stems. Tissue-specific expression of the gene showed that higher levels of the BcTuR3 transcript were observed in the TuMV-infected leaves, and later in the stems and roots of the resistant and susceptible varieties. These data showed that the BcTuR3 gene may be involved in plant resistance against TuMV pathogen infection.

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Abbreviations

TuMV:

Turnip mosaic virus

RACE:

Rapid amplification of cDNA ends

UTR:

Untranslated region

ORF:

Open reading frame

RT-PCR:

Real-time quantitative PCR

R-genes:

Resistance genes

SA:

Salicylic acid

SAR:

Systemic acquired resistance

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Acknowledgements

This study was supported by Innovative Scholars Project of Jiangsu Provincial Natural Science Foundation of China (No. BK2008035) and the earmarked fund for Modern Agro-industry Technology Research System.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Jingfan Ma, Xilin Hou, Dong Xiao, Li Qi, Feng Wang, Feifei Sun & Qian Wang

  2. Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing, 210095, People’s Republic of China

    Xilin Hou

  3. College of Life Sciences, Longyan University, Longyan, Fujian, 364000, People’s Republic of China

    Jingfan Ma

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  1. Jingfan Ma
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  2. Xilin Hou
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  4. Li Qi
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  5. Feng Wang
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Correspondence to Xilin Hou.

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Ma, J., Hou, X., Xiao, D. et al. Cloning and Characterization of the BcTuR3 Gene Related to Resistance to Turnip Mosaic Virus (TuMV) from Non-heading Chinese Cabbage. Plant Mol Biol Rep 28, 588–596 (2010). https://doi.org/10.1007/s11105-010-0183-3

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