Abstract
Background and aims
In sustainable agricultural systems, different beneficial soil microorganisms are explored to improve crop production and tolerance of plants to different environmental stresses. The present research aimed to examine the potential of plant beneficial rhizospheric microorganisms (PBRMs) for ameliorate the negative effects of salinity stress on sage (Salvia officinalis).
Methods
In this study, the effect of symbiosis with Piriformospora indica and Pseudomonas fluorescens in counteracting salinity stress on sage plants was quantified. The biomass production, physiological and biochemical markers and essential oil content and composition were evaluated under four salinity levels (0, 25, 50 and100 mM NaCl) in inoculated and non-inoculated sage plants.
Results
Fresh and dry herb yield, relative water content (RWC), chlorophyll and essential oil content exhibited a reduction in response to salinity. In contrast, salinity increased electrolyte leakage (EL), amount of malondialdehyde (MDA), accumulation of proline and total soluble sugars (TSS), activity of catalase (CAT), ascorbate peroxidase (APX) and guaiacol peroxidase (GPX) enzymes, total antioxidant activity, total phenol and flavonoids. Inoculation with microorganisms increased fresh and dry herb yield, RWC, chlorophyll content, accumulation of proline and TSS, antioxidant enzymes activity, total antioxidant activity, total phenol and flavonoids and essential oil content and decreased EL and amount of MDA. The dominant constituents of essential oils (α-thujone, β-thujone, camphor, 1,8-cineole, α-pinene, camphene and viridiflorol) showed changes in response to salinity stress.
Conclusion
The findings of this study showed that symbiotic association with PBRMs can help sage plants overcome the adverse effects of salinity stress.
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Data availability
The datasets generated during the current study are available from the corresponding author on reasonable request.
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Z. Aslani and A. Hassani, designed and carried out the experiment, and approved the final version to be submitted. B. Abdollahi Mandoulakani, participated in statistical analysis of the data. M. Barin, participated in preparing the inoculum of microorganisms and R. Maleki participated in the GC-MS analysis.
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Aslani, Z., Hassani, A., Mandoulakani, B.A. et al. The symbiotic association with Piriformospora indica and Pseudomonas fluorescens improves salt tolerance in sage (Salvia officinalis) plants. Plant Soil 495, 391–410 (2024). https://doi.org/10.1007/s11104-023-06334-7
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DOI: https://doi.org/10.1007/s11104-023-06334-7