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Effect of plant growth promoting Bacillus subtilis and Pseudomonas fluorescens on growth and pigment composition of radish plants (Raphanus sativus) under NaCl stress

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Photosynthetica

Abstract

Soil salinity is one of the most severe factors limiting growth and physiological response in Raphanus sativus. In this study, the possible role of plant growth promoting bacteria (PGPB) in alleviating soil salinity stress during plant growth under greenhouse conditions was investigated. Increasing salinity in the soil decreased plant growth, photosynthetic pigments content, phytohormones contents (indole-3-acetic acid, IAA and gibberellic acid, GA3) and mineral uptake compared to soil without salinity. Seeds inoculated with Bacillus subtilis and Pseudomonas fluorescens caused significantly increase in fresh and dry masses of roots and leaves, photosynthetic pigments, proline, total free amino acids and crude protein contents compared to noninoculated ones under salinity. The bacteria also increased phytohormones contents (IAA and GA3) and the contents of N, P, K+, Ca2+, and Mg2+ but decreased ABA contents and Na+ and Cl− content which may contribute in part to activation of processes involved in the alleviation of the effect of salt.

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Abbreviations

ABA:

abscisic acid

ACC:

1-aminocyclopropane-1-carboxylate

Car:

carotenoid

CAS:

chrom azurol S

Chl:

chlorophyll

DM:

dry mass

FM:

fresh mass

GA3 :

gibberellic acid

GC:

gas chromatography

HCN:

hydrogen cyanide

IAA:

indole-3-acetic acid

LSD:

least significant difference

PGPB:

plant growth promoting bacteria

PVK:

Pikovskaya

RT:

retention time

SD:

standard deviation

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  1. Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Roxy, Cairo, Egypt

    H. I. Mohamed & E. Z. Gomaa

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Mohamed, H.I., Gomaa, E.Z. Effect of plant growth promoting Bacillus subtilis and Pseudomonas fluorescens on growth and pigment composition of radish plants (Raphanus sativus) under NaCl stress. Photosynthetica 50, 263–272 (2012). https://doi.org/10.1007/s11099-012-0032-8

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