Abstract
Background and aims
Soil organic carbon (SOC) dynamics are vital in the context of climate change and sustainable soil management. The ẟ13C signatures of SOC are powerful indicators and tracers of C fluxes through soils and of transformation processes within soils. Depth gradients of ẟ13C can be considered as their archive. However, many different drivers and processes impact ẟ13C signatures of SOC simultaneously, thus hampering their interpretation.
Methods
Here we summarize the current knowledge about drivers, processes and C sources determining the δ13C signatures of organic matter along soil profiles.
Results
The largest ẟ13C gradients within soil profiles (> 10‰) have been observed at sites where vegetation has shifted between C3 and C4 plants, thus changing the isotopic signatures of C inputs. In soil profiles without such vegetation changes, the δ13C signatures typically increase by 1–3‰ from topsoil to subsoil. Three main reasons for this are (i) the decreasing ẟ13C of atmospheric CO2 (Suess effect) has led to a depletion in plant biomass by about 2.0‰ since 1850, (ii) increasing atmospheric CO2 concentrations have also depleted plant biomass by about 1.8‰, and (iii) isotopic fractionation occurs during continuous microbial C recycling and necromass accumulation. Moreover, a greater mobility of 13C-enriched hydrophilic dissolved organic C and other C input sources may impact ẟ13C gradients in soils.
Conclusions
External drivers, such as climatic and atmospheric changes, affect the ẟ13C signature of C inputs, and have stronger and increasing influence on ẟ13C gradients in soil profiles compared to soil internal processes.
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Introduction
Terrestrial carbon (C) pools and the underlying processes affecting their dynamics are important in the context of climate change mitigation, because ecosystems can act as both, sources and sinks of C. The future climate and functioning of ecosystems will be affected by the stability of the terrestrial carbon sink of which approximately 2270 Pg C is stored as soil organic carbon (SOC) in the upper two metres of the world’s soils (Jackson et al. 2017). This is equivalent to 200 years of current anthropogenic emissions (11 Pg C yr−1 2010–2019) (Friedlingstein et al. 2020; Mathieu et al. 2015). Isotopic analysis and approaches can elucidate the exchange of carbon between the pedosphere and the atmosphere (Metya et al. 2021), as well as the geosphere (Kalks et al. 2021), biosphere (Iversen et al. 2012) and hydrosphere (Hindshaw et al. 2016). In natural materials the abundance of the stable isotope 13C is between 0.96% and 1.16% of the C atoms, while the radioactive isotope 14C contributes < 10−12 % and the rest is 12C (Meija et al. 2016). The abundances of stable isotopes can be expessed by the δ13C annotation as the difference (in per mille, ‰) between the isotopic ratio (of heavy and light isotopes) of a sample and the isotopic ratio of a reference standard (e.g. for C Pee Dee Belemnite). The δ13C signature of C compounds is useful for tracing and quantifying recent C fluxes and pools, and can elucidate the origin and environmental conditions during the formation of the compounds up to a few thousand years ago (Boutton 1996; Ehleringer et al. 2000). Soil depth gradients in δ13C of SOC evolve as a result of internal and external drivers and processes, which are related to the soil C turnover, pedogenesis and the climate (Staddon 2004; Briones et al. 1999; Stevenson et al. 2005). δ13C depth gradients can elucidate palaeoecological developments and can help analysing mechanisms resulting in C storage changes (Krull and Retallack 2000; Diochon and Kellman 2008). The δ13C signature of SOC typically rises by 1–3‰ with depth in most soil profiles around the world (Fig. 1) (Wynn et al. 2005; Högberg et al. 2005; Boström et al. 2007; Ehleringer et al. 2000; Torn et al. 2002; Balesdent et al. 2018b; Dalal et al. 2005; Roscoe et al. 2001).
Typical patterns of the δ13C signature of SOC within soil profiles at sites with stable native vegetation and different climatic conditions. Mulga forest (Australia; 516 mm precipitation) (Dalal et al. 2005), Cerrado savannah woodland (Brazil; 1340 mm) (Roscoe et al. 2001), mature forest (Cameroon; 1560 mm) (Guillet et al. 2001) and wildland forest (Hawaii; 3000–4600 mm) (Bashkin and Binkley 1998). Reference is made here to the database of (Balesdent et al. 2018b) as a collection of soil ẟ13C depth gradients
There are several hypotheses that attempt to explain this phenomenon: (i) the influence of atmospheric or ecospheric changes, (ii) the preferential decomposition of different C components, (iii) the isotopic fractionation during the degradation of SOC, continuous necromass recycling and the distribution of fungal and bacterial biomass within soil profiles, (iv) the CO2 fixation and methane metabolism of microorganisms, (v) the input of root-derived C to the soil and (vi) the transport of dissolved organic carbon (DOC) (Boström et al. 2007; Ehleringer et al. 2000; Högberg et al. 2005; Nel and Cramer 2019; Bird et al. 2003; Kohl et al. 2015; Ni et al. 2020). Importantly, these drivers are not mutually exclusive and a number of them can simultaneously impact the δ13C signature, which makes it difficult to quantify their impact separately. Furthermore, the processes determining the inputs, development and isotopic fractionation of deep (> 30 cm soil depth) soil C have not been fully explored, despite that on average 47% of the SOC of the uppermost metre is stored at a depth of 30–100 cm (Mathieu et al. 2015; Ehleringer et al. 2000; Balesdent et al. 2018a). The slow turnover rates in the subsoil due to low microbial activity make it even more difficult to measure fractionation processes in situ (Mathieu et al. 2015). Even in the topsoil, measurement accuracy and the implementation of the gas sampling limits the detection of discriminations against 13C of < 0.5‰ during soil respiration processes (Boström et al. 2007). In soils with carbonates, these must be first removed without damaging the SOC. Otherwise soil inorganic C could overlay the depth gradient of the δ13C signature of SOC (Torn et al. 2002). Often not all the factors influencing the δ13C signature of SOC are known and considered, therefore interpretations of depth gradients are sometimes ambiguous. The following sections in this paper review the internal and external drivers, C sources and processes affecting the δ13C signature of SOC, and compile them into an overall picture to provide a basis for the interpretations of isotopic depth gradients (Fig. 2).
Internal and external drivers, carbon sources and processes affecting the δ13C signature of soil organic carbon. White boxes represent the carbon pools related to the pedosphere and the arrows show the fluxes between these pools. Affiliated keywords describe the drivers, processes and sources of carbon that have the potential to affect the isotopic signatures of bulk soil organic carbon. Plant graphics designed by Julia Schröder
External drivers and C sources affecting the ẟ13C signatures of SOC
The isotopic signatures of C inputs to the pedosphere are affected by numerous external environmental drivers influencing the previous photosynthetic C assimilation, and depend on the biological and geological sources of the C compounds. The following subchapters discuss the importance of atmospheric and other environmental drivers to the isotopic signatures of plant C assimilates and the subsequently formed soil organic C, as well as the impact of changing compositions of C sources, which are also affected by environmental conditions.
Drivers affecting the ẟ13C signatures of plant-assimilated C
As plant biomass is the main source of SOC, factors controlling the fractionation of 13C during photosynthesis influence the isotopic signature of SOC. During photosynthesis, plants discriminate against 13C isotopes driven by the diffusion resistance of their stomata and photosynthetic enzymes, or rather by the reaction rates between CO2 and Ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) during carboxylation processes (Schweizer et al. 1999; Bowling et al. 2008). The isotopic difference of the produced plant tissue and the consumed CO2 (isotopic discrimination) can be estimated for C3 plants using the following equation (Farquhar et al. 1989):
where the constants a and b represent the fractionation of 13C during diffusion through the stomata and the net fractionation during carboxylation predominantly by RuBisCO, respectively. The variables pi and pa are the intracellular and ambient partial pressure of CO2 respectively. The discrimination against 13C during photosynthesis generally provokes more negative isotopic signatures of plant tissues and SOC than those of atmospheric CO2, but does not lead to depth gradients of the isotopic signature within soil profiles by itself when the δ13C signature of plant C input to the soil are stable. Atmospheric drivers and changing environmental conditions over time, which modify the isotopic signature of photosynthetically assimilated C, can contribute to depth gradients of the δ13C signature of SOC owing to the larger amount of newer material in the topsoil than in the subsoil (Fig. 3a) (Balesdent et al. 2018a). The transport of decomposed and transformed carbon compounds to the subsoil and the addition of fresh litter inputs to the topsoil let 14C age gradients evolve. The mean age of SOC in global topsoils (0–30 cm) is around 1.4 kyr calculated by compiling global radiocarbon profiles, while the SOC in subsoils (30–100 cm) is on average 8.3 kyr old (Shi et al. 2020). Nevertheless, fresh carbon assimilates can have a visible impact on the δ13C signatures of SOC, especially in the topsoil. For example, a decade of experimentally CO2-enriched atmospheric conditions with depleted isotopic signatures led to a significant increase of the depth gradient of the δ13C signature of SOC (Fig. 3b) (Iversen et al. 2012).
a Global estimate of the distribution of the last 50 years of carbon inputs (1965–2015) within soil profiles. Data originate from (Balesdent et al. 2018a). b Depth gradient of the δ13C signature of SOC under a sweetgum plantation with Free-Air Carbon dioxide Enrichment (FACE). The blue line shows the depth gradient of δ13C after the plants have been grown in an atmosphere with 565 ppm CO2 and a depleted signature of δ13C = − 51‰ for eleven years, and the grey line represents the reference soil without manipulation. Data adapted from (Iversen et al. 2012)
Changes in the ẟ13C signatures of atmospheric CO2
The δ13C signature of the atmospheric CO2 is about − 8.7‰ today, which is 2.0‰ less than in 1850 before industrialisation (Fig. 4) (Rubino et al. 2019; Keeling et al. 2001). The decreasing δ13C signature is caused by the combustion of isotopically depleted fossil fuels (so called Suess effect) and is transmitted through plants into the SOC (Graven et al. 2017; Högberg et al. 2005). The impact on ẟ13C gradients in soils by the Suess effect depends on the soil type, the ratio of fresh C inputs to long-term stabilised C, the translocation of newly produced C into deeper soil layers, and the general C turnover time (cf. Fig. 3).
a Atmospheric CO2 concentration and δ13C signature from 1000–2022. Atmospheric CO2 records since 1959 (grey diamonds) based on yearly averages of direct observations from Mauna Loa (Hawaii) and the South Pole by the Scripps Institution of Oceanography (SCRIPPS) (Keeling et al. 2001). Furthermore, average δ13C signatures since 1980 derived from monthly seasonally adjusted data based on flask air samples from Mauna Loa (orange dots). The older data of the atmospheric CO2 concentration (black diamonds) and the δ13C signature (orange dots) originate from Law Dome ice core records generated by Rubino et al. (2019). b Correction factors for δ13C signatures and calculated δ13C signatures of mean C3 plant tissues produced during the last 2000 years related to increasing atmospheric CO2 concentrations (pCO2) (black/grey line), decreasing δ13C signatures (orange/rose line), and their combined effect (blue/dark blue line). For the δ13C signatures since 1980, seasonally-adjusted values were chosen (Keeling et al. 2001). The net isotopic difference between the atmospheric CO2 and the developed C3 plant tissue was approximated in relationship to changes in pCO2 using an equation from Schubert and Jahren (2015): \(\varDelta \delta^{13}C=\left[28.26*0.22(p{CO}_{2}+23.9)\right]/\left[28.26+0.22(p{CO}_{2}+23.9)\right].\)The isotopic signature of plant tissues at different points in time was calculated by: \(\delta {}^{13}{C}_{p}=\left[\left({R}_{a}/\left(\varDelta \delta{}^{13}C/1000+1\right)\right)/{R}_{VPDB}-1\right]1000\). Ra and RVPDB are the isotopic ratios of the atmospheric CO2 and the reference standard, respectively. For the correction only of changes of pCO2 the δ13C signature of 1019 was converted into a constant Ra, while the changing δ13C signatures of atmospheric CO2 were used for the combined effect (changing pCO2 and δ13C). The correction value was always calculated by \(\delta {}^{13}{C}_{p\left(t = x\right)}- \delta {}^{13}{C}_{p\left(t = 0\right)}\)
The δ13C signature of the atmospheric CO2 also changed in the Holocene before anthropogenic modifications, thus in the time period of pedogenesis of many soils. The CO2 of the glacial atmosphere during the Last Glacial Maximum had a δ13C of -7.1‰, which rose to a mean value of − 6.4‰ in the Holocene (Marino et al. 1992). During the Holocene, the δ13C signature of atmospheric CO2 increased by about 0.25‰ during the first 5 kyr before present (BP), and decreased by 0.05‰ after 6 kyr BP (before anthropogenic influence), driven by changes in the terrestrial biosphere and the marine carbonate cycle (Elsig et al. 2009). In particular, terrestrial biomass production and the uptake of isotopically light CO2 by plants grew during the early Holocene, which promoted an increasing atmospheric δ13C signature (Eggleston et al. 2016; Elsig et al. 2009). Most soils in the northern latitudes are younger than 12 kyr BP because ice advances have displaced older material and left periglacial deposits or bare bedrock material (Mathieu et al. 2015). Isotopic signatures of SOC produced during initial soil development in the early Holocene (SOC older than 6 kyr) should be more depleted than the signatures of SOC of preindustrial times, and could act as a small driver against the generally increasing δ13C signatures. However, the most pronounced changes in the isotopic signatures of plant assimilates have been observed recently due to the continuously increasing rate of anthropogenic CO2 emissions.
Changes in the concentration of atmospheric CO2
Atmospheric CO2 concentration changes throughout the planet’s history affects the isotopic signature of plants and therefore also SOC (Fig. 4) (Canadell et al. 2021; Graven et al. 2020; Farquhar 1991). The speed of the increase in atmospheric CO2 concentration between 1900 and 2019 was at least ten times faster than at any other point of time in the last 800 kyr (Canadell et al. 2021). This is a unique driver of temporal changes in the isotopic signatures of C inputs into the pedosphere. Increasing atmospheric CO2 concentrations (pa) are accompanied by decreasing isotopic signatures of the plant tissues because the amount of active carboxylation sites on RuBisCO and the carboxylation efficiency are increased, so that a larger amount of CO2 entering the leaves is usable for fixation (Schubert and Jahren 2012; Gamage et al. 2018). Besides, the stomatal conductance of the plant leaves decreases when the pCO2 rises, which results in a lower pi/pa ratio and higher δ13C signatures of C assimilates (see Eq. 1) (Farquhar et al. 1982; Gamage et al. 2018). Another study claims that mostly changing fractionation factors of the photorespiration and the conductance of the mesophyll lead to increasing isotopic signatures of plant tissues of about 0.014‰ ± 0.007‰ ppm−1 (Keeling et al. 2017). Others predict a hyperbolic relationship between changes in pCO2 levels and in fractionation during photosynthesis, with larger modifications in the degree of isotopic fractionation (> 0.01‰ ppm−1) when low pCO2 levels (≪ 450‰) experience changes (Schubert and Jahren 2012, 2015). During the later Holocene, after 8 kyr BP the atmospheric pCO2 increased from 260 ppm to 280 ppm (preindustrial times) (Elsig et al. 2009; Joos et al. 2004). The rise of pCO2 during the last deglaciation has been calculated to cause a decrease in the δ13C signature of 1.4‰ ± 1.2‰ for gymnosperms and 0.5‰ ± 1.5‰ for angiosperms (Hare et al. 2018). A global increase of about 2.1‰ of the discrimination factor against 13C of C3 plants has been computed by analysing δ13C data from fossil leaves and bulk terrestrial organic matter from the last 30 kyr and explained by a rise in pCO2 by 80 ppm from the Last Glacial to preindustrial times (Schubert and Jahren 2015).
All in all, the decreasing isotopic signature of atmospheric CO2 (Suess effect) and its increasing concentration (pCO2) have an important impact on the δ13C signature of SOC because each has led to a depletion in the isotopic signature of new plant biomass inputs by about 2.0‰ and 1.8‰ respectively since industrialisation (1850) (calculated with data and equations from (Keeling et al. 2001; Rubino et al. 2019; Schubert and Jahren 2015) (Fig. 4). A tree ring chronology displays decreasing values of about 3.5‰ between 1850 and 2005, probably because of the increasing pCO2 and decreasing δ13C signature (Wang et al. 2011). Archived samples from wheat continuously grown on the same unfertilised field similarly show a significantly decreasing trend in the δ13C signature over 153 years (1845–1997), which corresponds to the decreasing signature of atmospheric CO2, especially after 1960 (Zhao et al. 2001). The δ13C signature of the grain and straw of wheat decreased by about 2.8‰ and 2.5‰, respectively, whereas the signature of global atmospheric CO2 decreased by only about 1.5‰ over that period. The remaining change can be explained by the increasing discrimination against 13C because of rising pCO2, alternating wheat cultivars and climatic changes of the precipitation amount and the temperature.
Changes in the environment
There are several factors influencing the δ13C of plants through their effect on the pi/pa ratio (see Eq. 1), which is altered by the rate of diffusion of CO2 into the leaves or the rate of CO2 consumption by photosynthesis (Boutton 1996). Important environmental factors include irradiance, atmospheric pCO2 (see Changes in the concentration of atmospheric CO2 section), vapour pressure deficit, soil moisture availability, soil salinity, soil fertility, air pollution, altitude, latitude and exposition, while biological factors include photosynthetic capacity, plant development status, genetic variation, competition, growth form and life span (Fu et al. 1993; Boutton 1996; Kohn 2010). For example, the δ13C signatures of plant biomass increase with better nitrogen (N) availability, because the intercellular CO2 consumption is higher and the pi/pa ratio is lower when C-fixing enzymes and photosynthetic capacity are promoted by higher N contents in the leaves (Fu et al. 1993). Furthermore, the δ13C signature of C3 plants is strongly correlated with mean annual precipitation because the discrimination against heavy isotopes is lower when stomata have to close more often to minimise water loss and the intercellular concentration of CO2 (pi) decreases (Madhavan et al. 1991; Farquhar et al. 1989; Kohn 2010). Isotopic δ13C signatures of C3 plants above − 25.5‰ can only be achieved in present environments with precipitation amounts < 500 mm yr−1 when plants experience water stress more often (Kohn 2010). A study observed decreasing δ13C signatures (under less arid conditions) of − 0.6‰ 100 mm−1 for C3 plants and − 0.3‰ 100 mm−1 for C4 plants sampled along a precipitation gradient (25-600 mm) with an isotherm (Ma et al. 2012). They were also able to observe a related decrease in the isotopic signature of SOC (of the upper 2–3 cm) by about − 0.4‰ 100 mm−1 for the precipitation range. Others found the δ13C signatures of C3 plants grown in 75% air humidity to be 1.8–2.6‰ lower than in 30% air humidity, while C4 plants showed 0.8‰ higher isotopic signatures under moister conditions (Madhavan et al. 1991). For some C4 vegetation and plant species, no or a postive correlation with precipitation can be found (Ma et al. 2012; Swap et al. 2004). However, a substantial shift of up to 15‰ higher δ13C signatures can occur when particular C3 plant species change their photosynthetic pathway to CAM (Crassulacean acid metabolism) in response to drought (Farquhar et al. 1989; Bloom and Troughton 1979). The latter is only of small and localised importance when these species are abundant. Increasing precipitation amounts, for example in Europe and western North America (Herzschuh et al. 2022) or in several regions of low latitudes (Braconnot et al. 2007; Rao et al. 2012) during the Holocene, could result in decreasing isotopic signatures of plants and formed SOC, which can contribute to local depth gradients of increasing δ13C signatures of SOC. Recently, climate change has increasingly been reducing water availability in many parts of the world, which leads to higher δ13C signatures in plants (Canadell et al. 2021; Farquhar et al. 1989). This can be a future driver working against the commonly observed increasing δ13C signatures of SOC with depth.
Sources of C inputs with different ẟ13C signatures
The main input source of new SOC to the pedosphere are autochthonous plant assimilates that are released from plants during their decomposition and as root exudates or are exported to their mycorrhizal fungal partners. The ẟ13C signatures of these C inputs depend on the photosynthetic pathway the plants use and on the plant organ which gets decomposed. Furthermore, organic carbon can enter the soil not only directly from plants, but also as allochthonous carbon due to anthropogenic activity or natural lateral transport. Furthermore, C can reach soils from the underlying parent material through weathering processes as geogenic C, from landscape fires as pyrogenic C, or through dark microbial C fixation.
C3 and C4 plant-assimilated C
Vegetation type, cropped plant species and agricultural practices change perpetually over time and affect the isotopic signature of SOC in soil profiles in many terrestrial systems (Basile-Doelsch et al. 2009; Trouve et al. 1994; Pessenda et al. 2010). Enrichments or depletions of δ13C signatures of more than 10‰ can be found between surface soil and deep soil layers, probably due to temporal changes in the vegetation or, more precisely, the proportion of C3 and C4 plants (Fig. 5) (Martinelli et al. 1996; Mariotti and Peterschmitt 1994; Desjardins et al. 1991; Pessenda et al. 2010; Krull et al. 2005; Schneckenberger and Kuzyakov 2007; Trouve et al. 1994). The photosynthetic pathway of C3 plants leads to isotopic signatures of their biomass of between δ13C = − 20‰ and − 37‰, whereas C4 plants show signatures of between δ13C = − 9‰ and − 17‰ (O’Leary 1988; Boutton et al. 1998; Kohn 2010). The mean isotopic difference between C3 and C4 plants is approximated to be about 14‰ (− 27‰ vs. − 13‰), which is most clearly translated into δ13C soil depth gradients when there is a complete vegetation change (C3/C4 or C4/C3) and the SOC in the topsoil mostly consists of fresh C assimilates in contrast to the subsoil.
Depth gradients of δ13C signatures within soil profiles at sites with and without vegetation changes. Coloured lines show soil profiles after a vegetation change from C3 to C4 plants (a and b) or from C4 to C3 (c). a is drawn with data from (Pessenda et al. 2010) for two adjacent soil profiles in Brazil under a rainforest (REBIO V) (grey) and an open arboreal savannah ‘Tabuleiro’ (REBIO III) (yellow), which was previously a rainforest. b is created with data from (Schneckenberger and Kuzyakov 2007) for soil profiles in Germany under a grassland (grey) and after 9 years of Miscanthus cultivation (green). c originates from (Trouve et al. 1994) and shows adjacent soil profiles in Congo under a savannah (grey) and a 21-year-old Pinus plantation (brown)
The δ13C signature of SOC reflects the relative contribution of C3 and C4 vegetation to the community net primary productivity, and can serve as a proxy indicator of the development of vegetation and climate over time, because the geographical distribution of C4 plants is correlated with temperature and precipitation (Boutton et al. 1993; Osborne and Sack 2012; Rao et al. 2010). The main natural expansions of C4 plants in low and middle latitudes have occurred since the late Miocene (Rao et al. 2012) as an adaption to improve the water and carbon acquisition of plants in an environment with decreased pCO2 compared with the preceding Paleogene (Osborne and Sack 2012). C4 plants are better adapted to higher temperature environments, however an increase in pCO2 in particular promotes C3 plants (Ehleringer and Cerling 2002). Rao et al. (2012) conclude that during the Holocene the proportion of C4 vegetation often decreases at low latitudes because of increasing precipitation, while at middle latitudes the proportion of C4 vegetation increases because of rising temperatures. The vegetation changes at low latitudes can be a factor leading to greater increases in the δ13C signatures of SOC with depth, while at middle latitudes these changes can attenuate isotopic depth gradients. However, since changes in the precipitation amount and temperature during the Holocene are not uniform at different latitudes and on different continents (Braconnot et al. 2007), their influences on the vegetation and thus on the depth gradient of the δ13C signature of SOC must be individually assessed for each site. Currently C4 plants account globally for approximately 20% of terrestrial gross primary production (Beer et al. 2010) and have their highest abundances on savannahs and grasslands between 30–40 °N and 15–25 °S (Bird and Pousai 1997; Rao et al. 2010). At high latitudes above 60 °N and below 46 °S, C4 plants are and have mostly been absent because the temperatures are too low (Rao et al. 2012), so natural vegetation changes should have minor effects on the depth gradients of δ13C signatures there. The vegetation also changes due to human activities (e.g. arable farming, forestry or livestock) (Boutton et al. 1993; Basile-Doelsch et al. 2009). For example, livestock grazing can promote C3 grass over C4 vegetation in tallgrass prairie and can result in more depleted δ13C signatures of the SOC than on ungrazed sites in the upper 90 cm of soil (Boutton et al. 1993). In contrast, the introduction of C4 plants such as maize in arable fields at higher latitudes is a factor leading to higher isotopic signatures of newly formed SOC and lower δ13C gradients within soil profiles (Schneider et al. 2021).
Above- and belowground C inputs
The average maximum rooting depth of different biomes ranges from 0.5 m for tundra to 15 m for tropical grassland and savannah (Canadell et al. 1996) and determines the location of belowground C inputs. The rooting depths of different plant species could affect the δ13C signatures within soil profiles if their isotopic signatures of the rhizodeposits deviate from that of above ground litter. The roots of C3 plants commonly show enriched isotopic signatures of about 1.2‰ compared to the leaves, while the roots of C4 plants often have smaller enrichments of 0.4‰ (Bird et al. 2003; Werth and Kuzyakov 2010). It was demonstrated that the respired CO2 of C3 plant leaves and of the roots of woody species is commonly enriched in 13C, while the respiration of the roots of herbaceous species is commonly depleted; both leading to post-photosynthetic fractionations (Ghashghaie and Badeck 2014). This could promote an enrichment of the roots of herbaceous C3 plants compared to their leaves besides the influences of deviating biochemical compositions, diel carbohydrate dynamics and other possible factors reviewed by Cernusak et al. (2009). A rapid rise of the δ13C signature of 0.5-1.0‰ with increasing soil depth within the upper 10 cm could be therefore promoted by an increasing proportion of root-derived C and a lower above ground litter derived fraction (Bird et al. 2003) along with effects of preferential degradation. Additionally, vegetation changes can result in variations in the depth of C inputs. Especially in the boreal and temperate zone, there was a succession of tree species across the landscape after the last ice age until it was mostly forest-dominated. It could be assumed that ancient root-derived carbon in the subsoil can evoke a difference between the isotopic signatures of the SOC in the top- and subsoil. But as cold and temperate forest ecosystems show higher isotopic discriminations during photosynthesis than temperate grasslands (Kaplan et al. 2002), such vegetation changes might be a driver against increasing δ13C signatures with depth.
Pyrogenic C
Another C input to soils is pyrogenic carbon, which is produced by the burning of biomass. Around 12% of emissions from landscape fires are buffered by the production of pyrogenic C, which accumulates in soils because of its high recalcitrance (Jones et al. 2019). Pyrogenic C comprises on average 14% of total SOC and clay-rich soils can even store > 30% of total SOC as pyrogenic carbon (Reisser et al. 2016). The isotopic signature of pyrogenic C largely reflects the signature of the source, but can deviate by about ± 2‰ depending on the burning temperature (Bird and Ascough 2012). An effect of pyrogenic C on the isotopic signature of SOC only appears in environments where fires are frequent at least during one time interval of pedogenesis. Soil δ13C depth gradients could be affected if the proportion of pyrogenic C to bulk SOC changes within the soil profile and the isotopic signature of pyrogenic C differs from that of the bulk SOC.
Dark microbial fixed C
Most microorganisms in the soil are chemoorganoheterotrophs and use organic molecules as their C and energy source (Kästner et al. 2021; Delgado-Baquerizo et al. 2018). However, besides photoautotrophs certain prokaryotic taxa are capable of directly fixing inorganic C chemoautotrophically or anaplerotically, which might result in δ13C signatures of the biomass of the latter closer to the atmospheric source than photosynthetically assimilated C (Nel and Cramer 2019). The enzymes active in the anaplerotic pathway mainly accept the inorganic C phase of HCO3−, which can be substantially enriched in 13C by up to 7–11‰ in comparison with CO2 because of the temperature-dependent fractionation during its hydrogenation (Mook et al. 1974; Zhang et al. 1995). Such anaplerotic fixation can lead to higher δ13C signatures in microbial biomass and is further influenced by the fraction of atmospheric CO2 in the upper soil layers, which has a higher 13C content than soil CO2 derived from respiration (Nel and Cramer 2019). Furthermore, the soil CO2 around the soil organism is enriched in 13C in comparison with soil-respired CO2 due to the different diffusion coefficients of 13CO2 and 12CO2 out of the soil, which can result in a depletion of soil-respired CO2 of 4.4‰ (Cerling et al. 1991). The fixation of inorganic C by some microorganisms can be used to maintain metabolic activity under C-poor conditions (Šantrůčková et al. 2018). The rates of dark microbial C fixation are small compared with soil respiration rates (1.2–3.9% of the respiration) and even with the fixation rate of photoautotrophic soil surface microbes (Spohn et al. 2020; Delgado-Baquerizo et al. 2018; Schmidt et al. 2016). Dark microbial C fixation could increase δ13C signatures of SOC in particular environments where heterotrophic metabolism is slow or where inorganic C provides the dominant basis for the microbial community, for example in deep arctic or hyper-arid soils (Šantrůčková et al. 2018; Ji et al. 2017).
Allochthonous C
Anthropogenic modifications, such as deep ploughing, landfills, plaggen soil and mining, shift and bury large amounts of topsoil C into the subsoil (Schneider et al. 2021). Historic heathland and peatland cover of agricultural soils influences the quality, quantity and isotopic signature of the stored SOC (Schneider et al. 2021). Another allochthonous input of C into the soil system is the use of organic fertilisers. Animal manure can be depleted in comparison with animal feed (plant materials) by about 0.3‰ (Bol et al. 2005; Kristiansen et al. 2004). A significant decrease in the δ13C signature of SOC in the topsoil was observed after 37 years of manure application because it had a lower δ13C signature than the original SOC (Gerzabek et al. 1997). Another long-term field experiment with different fertilisation regimes found the C content and δ13C signature of SOC in the upper 20 cm to be similar, irrespective of the usage of animal manure or mineral fertiliser, probably because the amount of the manure was much smaller than the residues of the crops (Bol et al. 2005). It can be concluded that organic fertilisers only change the δ13C signature of SOC when the applied amount is high and the isotopic signature of animals’ diet differs significantly from that of crops on the field.
Geogenic C
Geogenic organic carbon (GOC) is bedrock derived C and can affect the SOC isotopic signature in the subsoil. Sedimentary bedrocks can contain 0.1–0.3 g kg−1 GOC and are widespread over 65% of the terrestrial earth surface (Amiotte-Suchet et al. 2003; Kalks et al. 2021). The effect on the SOC isotopic signature is determined by the amount of the GOC in the bedrock, the rate of turnover of this GOC, the quantity of SOC, and the difference between the isotopic signatures of SOC and GOC (Kalks et al. 2021). GOC potentially has a δ13C signature different to that of modern vegetation because of lower pCO2, higher δ13C signatures of atmospheric CO2 and climate conditions during C fixation that differ from those found today. Allochthonous C in colluvial or alluvial Holocene deposits from upstream or upslope can enhance C amounts in subsoils substantially, while C from aquatic sources can lead to depleted isotopic signatures of organic C in Holocene parent materials (Schneider et al. 2021). If C compounds are incorporated into sediments under marine conditions, the isotopic signature of plankton is the most important, while under continental conditions the detritus of higher plants affects the isotopic signature of the organic C in the sediments (Deines 1988). Carbon in sedimentary rocks has been termed kerogen, and consists of coal-like particles of amorphous C formed by compaction and dewatering during diagenesis. The isotopic signature of these kerogens is not only influenced by the C sources, but also by the fractionation due to the transformation from plant tissue to organic matter and diagenetic changes in the sediment (Deines 1988).
Internal processes affecting the ẟ13C signatures of SOC
Organic C compounds become decomposed, transformed, translocated and stabilised within the soil profile by soil-internal processes (see Fig. 2). Carbon components with various chemical structures, physical shapes (of particulate organic matter) and isotopic signatures can be translocated and stabilised along the soil profiles. When new organic material enters the soil, the balance between microbial priming and microbial entombing affects the organic material’s fate in the soil (Liang et al. 2017). The influence of a preferential decomposition (catabolism) and physico-chemical stabilisation of SOC on compounds with specific isotopic signatures and the effects of microbial biomass synthesis (anabolism) are discussed below. Furthermore, the transport of DOC is discussed in relation to its impact on the isotopic signature of SOC.
Preferential C turnover and physico-chemical stabilisation
Preferential C turnover
During initial decomposition stages (leaf senescence, litterfall and SOC formation) an enrichment of the remaining organic material with 13C by 1.7–2.9‰ was observed irrespective of the vegetation type (Buchmann et al. 1997). This is supported by the observation of depleted isotopic signatures of evolving CO2 during the initial decomposition processes (Kristiansen et al. 2004). In contrast, other incubation experiments found for initial decomposition stages respired CO2 from plant litter to be depleted 13C in comparison with the litter but to be enriched after more than a week of incubation (Schweizer et al. 1999; Fernandez et al. 2003). The depletion of the respired CO2 in the first stage of the initial decomposition could be because of a kinetic fractionation effect by highly active microbes, when the energy availability and nutrient status of the C compounds are high (Feng 2002; Fernandez et al. 2003). It could likewise be related to a turnover of microbial biomass if the microbes have used depleted C sources compared to the freshly added substrate prior to the addition (Blagodatskaya and Kuzyakov 2008). In the later stage of the initial decomposition of litter, the preferential usage of enriched substrates such as cellulose or enriched microbial necromass could be responsible for the observed increase in the isotopic signature of the respired CO2 (Fernandez et al. 2003; Prescott and Vesterdal 2021). All in all, the changing δ13C signatures of the remaining C compounds during the initial decomposition stages can be explained by time-dependent opposing factors: enrichment by the kinetic isotope fractionation of microbial respiration and depletion caused by preferential decomposition (Balesdent and Mariotti 1996; Feng 2002; Fernandez et al. 2003). In general aging of organic matter results in 13C enrichments. However, others have observed non-directional changes of < 0.5‰ in the isotopic signature of leaf litter from tree and shrub species during the first year of decomposition although half of the litter mass was lost (Boutton 1996). Carbon decomposition and transformation was even investigated in long-term bare fallow experiments that did not receive any fresh litter input. An increase of 0.4‰ in the δ13C signature in the topsoil SOC was found when soils lay bare without vegetation for 37 years (Gerzabek et al. 1997). Another long-term experiment with bare fallow found an enrichment in 13C of 0.008–0.024‰ per year and an absolute increase of 2.0‰ in an 80-year period, during which 65% of the SOC was lost (Menichetti et al. 2015). An enrichment of the remaining material in 13C is in line with the observed trend of increasing δ13C signature with soil depth, because the proportion of transformed C compounds is higher in the subsoil than in the topsoil.
Different parts of plant materials, litter fractions and SOC compounds deviate in their δ13C signature relative to the bulk material, which can have an effect on the isotopic signature of the remaining SOC and respired CO2 when a preferential turnover of selected compounds takes place (Benner et al. 1987; Schweizer et al. 1999; Bowling et al. 2008). Several plant tissues show different isotopic signatures, for example the roots of legumes are enriched by about 1.5‰ in comparison with their leaves (Schweizer et al. 1999) and grains of wheat show on average a 1.9‰ higher isotopic signature than straw (Zhao et al. 2001). Isotopic signatures often deviate because of the distinct chemical compounds that form the cell structures of different plant tissues. Lipids are depleted in 13C while pectin tends to be enriched (Deines 1988). The cellulose and hemicellulose fractions are enriched by 1–2‰ relative to the whole plant material, whereas the lignin fraction is commonly depleted by 2–6‰ in comparison with the bulk material (Benner et al. 1987; Schweizer et al. 1999). Selective preservation of lignin is mostly relevant in the initial decomposition stages of litter (Kalbitz et al. 2006), but the chemical composition does not determine the long-term fate in soils (Marschner et al. 2008; Heim and Schmidt 2007; Prescott and Vesterdal 2021). Thus, the initial stages of litter decomposition provide few insights into the long-term persistence of organic compounds and the development of the isotopic signatures of C deeper than the litter layer. Physical protection, chemical interactions of organic compounds with mineral surfaces and biological factors determine the stabilisation, turnover and transformation of SOC (Schmidt et al. 2011; Marschner et al. 2008), and thus impact the δ13C signature of SOC. In the long term, microbial C recycling could play a greater role and contribute to increasing δ13C signatures with time and depth (see Microbial C recycling section) (Balesdent and Mariotti 1996). Differentiation between a preferential usage of C compounds and fractionation effects during microbial metabolism remains a challenge.
Physico-chemical stabilisation of C in the soil profile
The chemical structure of organic molecules is found to be important only during early decomposition and not for long-term stabilisation, because most SOC of natural origin can be metabolised by highly adapted microbial communities (Dungait et al. 2012; Lützow et al. 2006; Schmidt et al. 2011; Ruiz-Dueñas and Martínez 2009). Specific chemical structures and compounds may, however, influence its turnover, such as pyrogenic C from incomplete combustion (see Pyrogenic C section) or plant phytoliths (PhytOC) that have promoted C stabilisation for hundreds to millions of years (Lorenz et al. 2007; Parr and Sullivan 2005; Kirschbaum et al. 2021). Compounds can resist mineralisation processes when they provide a small energetic yield relative to the energy amount required for synthesis of the enzymes targeting them (Henneron et al. 2022). Plant phytoliths are plant opals containing silica and on average 1% C, and can be stabilised in soils because of their high recalcitrance, with accumulation rates of around 18 g C m−2 yr−1 in sugarcane fields for example (Parr and Sullivan 2005; Basu et al. 2015). The isotopic signature of newly formed PhytOC is depleted by about 13‰ in comparison with the source plant in the case of C4 plants and show nearly the same signature as the plant when a C3 plant is the source (Basu et al. 2015). In most soils, however, the amount of PhytOC is expected to be too small to impact the bulk SOC isotopic signatures, especially in the topsoil.
Physical inaccessibility to microbial communities through occlusion within aggregates, organo-mineral interactions and relocation in deeper soil horizons, which are often waterlogged, anoxic, cold and nutrient-limited, determine the long-term stabilisation of C (Kopittke et al. 2018; Dungait et al. 2012). Carbon compounds reaching deep soil layers are probably better protected from decomposition because the rate of microbial catabolism decreases substantially with depth (Fontaine et al. 2007). The soil texture can determine the SOC turnover rate, whereby coarse-textured soils demonstrate faster turnover rates (Boutton et al. 1993). This can ultimately affect the steepness of the δ13C depth gradient. For example, in a coarse-textured soil, the δ13C signature of the SOC increased by 1.3–1.6‰ from the topsoil to 100 cm soil depth compared with a fine-textured soil with a much larger δ13C gradient of 3.8–5.8‰ (Wynn et al. 2005). Mineral-associated organic carbon (MAOC) was found to originate more from fungal residues than from plant residues (Klink et al. 2022) and to have a 1–3‰ higher δ13C signature than the plant C inputs (Torn et al. 2009). Kinetic fractionation during decomposition of C compounds may result in preferential accumulation of 13C. The higher the degree of stabilisation, the longer the transformation time and ultimately thus the higher the 13C signature (Wynn et al. 2005). The stabilisation of higher amounts of 13C−enriched microbial necromass in fine-textured soils could be a reason for the stronger gradients in these soil types (see Microbial C recycling section) (Ni et al. 2020).
Microbial C recycling
Microorganisms mediate both the mineralisation and formation of SOC (Kästner et al. 2021). Just a slight fractionation during microbial transformation processes can cause a significant enrichment of the remaining SOC since the C fraction remaining in the soil is small compared with the total C inputs (Högberg et al. 2005). Most studies have found the biomass of microbes and other soil biota to be enriched by > 2‰ relative to the SOC in the topsoil due to anabolic processes (Dijkstra et al. 2006; Šantrůčková et al. 2000; Gleixner et al. 1993; Potthoff et al. 2003; Briones et al. 1999). While the microbial biomass comprises less than 5% of the SOC (Dalal 1998), the necromass (dead microbial biomass) contributes about 51, 47 and 35% to the SOC in the topsoil of croplands, grasslands and forests, rspectively (Wang et al. 2021). Microbial recycling of building blocks (e.g. amino acids, amino sugars, mono- and oligomers of fatty acids, nucleotides) from microbial necromass could provide the resources for microbial growth cycles (Kästner et al. 2021). This is expected to result in steady 13C enrichment during the continuous recycling of microbial necromass, which is preferentially used because it is energetically favourable to incorporate pre-existing compounds to biomass rather than synthesise new compounds. Even though the abundance of microorganisms is often higher in the topsoil than in the subsoil (Hicks Pries et al. 2018), their proportion of SOC is smaller in the topsoil because of the high input of plant-derived C compounds. Therefore, the impact of the microbial biomass and necromass on the δ13C signature of the SOC is greater in the subsoil. Not only the proportion to SOC, but also the isotopic signature of microbial biomass changes within soil profiles promoting enrichments in 13C with depth. A study found increasing δ13C signatures of 7.6–8.4‰ of the microbial biomass (indicated by phospholipid fatty acids; PLFA) with soil depth (from organic L layer to the mineral B horizon) (Kohl et al. 2015). The concentration of PLFA and the isotopic signatures of specific fungal and bacterial PLFA were nearly constant over depth, but the fungi/bacteria ratio decreased. The latter can explain the increase in the δ13C signature of the PLFA within the soil profile in this case because the fungi showed lower δ13C signatures than the bacteria and the bulk SOC (Kohl et al. 2015). This could be caused by some fungi being specialised degraders of lignin that is 13C depleted (Osono 2007; Benner et al. 1987). Another study also found an increasing share of bacterial necromass in total SOC with depth from 12% at 0–20 cm depth to 39% at 50–100 cm depth, while the proportion of fungal necromass remains constant at between 19 and 23% (Ni et al. 2020). All in all, the bacteria-dominated microbial biomass and necromass in subsoils could promote the formation of the isotopic soil depth gradient with an increase of 2.8‰ (Kohl et al. 2015). Furthermore, land-use type can affect the fungi/bacteria ratio of the necromass, which is higher in forest topsoils than in arable soils and grasslands (Kohl et al. 2015; Liang et al. 2019). A higher δ13C signature in the subsoil could also be promoted by the leaching of microbial products as DOC (see Translocation of DOC section) and a higher relative contribution of microbe–derived C relative to plant-derived C (Boström et al. 2007; Ehleringer et al. 2000; Kaiser and Kalbitz 2012).
The C input via mycelia can be an important pathway for C entering the soil, and can exceed the input via litter and fine root turnover (Godbold et al. 2006). A study has found that on average more than 40% of the SOC consists of fungal necromass and only 15% of bacterial necromass in the upper 25 cm of agricultural and grassland topsoils (Liang et al. 2019). The δ13C signature of the biomass of saprophytic fungi can deviate by ± 2.5‰ from their substrates (Abraham and Hesse 2003). In a greenhouse experiment, the hyphae of arbuscular mycorrhizal fungi were observed to be enriched in 13C by 1–6‰ in comparison with their host plants (Walder et al. 2012). Fruit bodies of host-specific ectomycorrhiza had a 1.2–2.9‰ higher δ13C signature than the foliar samples from their host trees (Högberg et al. 1999). However, this is not necessarily a result of a fractionation process because ectomycorrhizal fungi are often facultative saprotrophs that utilise both plant and SOC-derived compounds for growth (Hobbie et al. 2001; Kuyper 2016). The effect of fungi on the isotopic signature of SOC within soil profiles remains equivocal, although fungal necromass is an important component of SOC (Liang et al. 2019). In general, the biomass of microorganisms and other soil biota tends to be 13C enriched relative to the SOC pool, with a direct influence on the depth gradient of the isotopic signature of SOC within soil profiles due to the increasing proportion of microbial recycled SOC with soil depth.
Methanogenesis and methanotrophy
The metabolism of methanotrophs and methanogens could have an impact on the isotopic signatures of SOC, especially in wet soils with anoxic layers. Methanogenic archaea generally live under anoxic conditions (Penger et al. 2012), but methanogens also exist in oxygenated soils, probably located within anaerobic microsites of soil aggregates (Angle et al. 2017). In general, methane is produced by archaea in deep, anoxic soil layers and reaches δ13C signatures of -55.9 ± 4.2‰ in rice paddies for example (Uzaki et al. 1991). Methylotrophic methanogens produce CH4 with a δ13C signature up to 80‰ lower than their substrate, whereas hydrogenotrophic methanogens show a fractionation of 22–58‰, and acetoclastic methanogens display a fractionation of < 5‰ against the model substrate acetate, all depending on temperature (Valentine et al. 2004; Penger et al. 2012). Reaction equations of the anaerobic degradation of carbohydrates predict that one third of the methane is produced by hydrogenotrophs fuelled by hydrogen and CO2, and two thirds by acetoclastic methanogens using acetate as a substrate (Conrad 1999), but the proportion of hydrogenotrophs to methane production can be even higher (Lansdown et al. 1992). Due to the strong discrimination against 13C during methane production by methanogens, the remaining unconsumed dissolved CO2 can reach signatures above 5‰ (Dorodnikov et al. 2022). It is possible that the biomass and necromass of the archaea are isotopically enriched and accumulate within anoxic soil layers. However, actual measurements show no trends of increasing δ13C signatures in deep anoxic peat (Rask and Schoenau 1993; Alewell et al. 2011), suggesting that additional factors are involved. It can be concluded that methanogenesis has a small overall influence on bulk SOC isotopic signatures due to low absolute amounts of produced methane in most soils, except for wetlands. But even in these ecosystems anaerobic mineralisation may barely affect the isotopic signatures of SOC, because of the huge overall SOC content, for example in peatlands.
The opposite process is methane oxidation, which occurs in most aerobic soils via methanotrophs (Le Mer and Roger 2001). These methane-oxidising bacteria (MOB) prevent emissions to the atmosphere from methanogens and also take up methane from the atmosphere, making soils an important biological methane sink (Le Mer and Roger 2001; Ho et al. 2019). Methanotrophs preferentially use light C isotopes of the already isotopically depleted methane (Barker and Fritz 1981). Extremely depleted δ13C signatures of − 42‰ are observed in Early Triassic paleosols, probably because of significant amounts of SOC derived from methanotrophic biomass during this time (Krull and Retallack 2000). Besides the conversion to CO2, about 7% of the consumed CH4 can be transformed into organic acids and 40% into microbial biomass (Khanongnuch et al. 2022), which could lowe the isotopic signature of topsoil SOC. The effects of methanotrops on the δ13C signature of SOC are generally low in most soils because of their small population sizes relative to heterotrophic microorganisms, even though fractionation effects are relatively large (Krull and Retallack 2000). All in all, methanogenesis and methanotrophy are processes that should promote steeper depth gradients of the δ13C signatures of SOC because of the upward translocation of 12C as depleted CH4. But in wetlands, where these processes are most powerful depth gradients are small (see Examples of ẟ13C gradients of several soil profiles and general interpretation guidelines section) (Alewell et al. 2011).
Translocation of DOC
Translocation of C from topsoil to subsoils mostly occurs by illuviation as dissolved organic carbon (DOC) or by bioturbation (Schneider et al. 2021). The DOC is typically derived from rhizodeposits, breakdown of SOC or products of microbial necromass (Trumbore et al. 1992). The mobility of DOC through the soil column is higher than that of particulate C because of its small size (< 0.45 μm), which decreases physical restrictions. Nevertheless, the transport of DOC within a soil profile is a complex process involving temporal immobilisation, microbial processing, subsequent desorption and dissolution (Kaiser and Kalbitz 2012). Philben et al. (2022) could not identify any significant difference between the isotopic signature of SOC and DOC percolation from the organic topsoil layer of a forest ecosystem and hypothesized that DOC does not impact the δ13C gradients within soil profiles. But others showed that in the topsoil, the amount of plant-derived DOC is higher, whereas in the subsoil microbe-derived DOC is more abundant and therefore often enriched in 13C (Kaiser and Kalbitz 2012; Högberg et al. 1999; Briones et al. 1999). Kaiser et al. (2001) further observed an accumulation of the hydrophilic DOC fraction with depth when water moves vertically through the soil profile. Hydrophilic DOC is enriched in 13C compared with the hydrophobic DOC fraction and leads to an enrichment in 13C with soil depth. Mineral surfaces preferentially sorb hydrophobic substances, such as depleted lignin-derived compounds, leading to an enrichment of the average δ13C signature of compounds remaining in the soil water (Kaiser et al. 2001). Changes to the isotopic signature of DOC are primarily due to the relative proportion of hydrophilic and hydrophobic compounds determined by their mobility, which could contribute to the enrichment of δ13C signatures of SOC with soil depth.
Examples of ẟ13C gradients of several soil profiles and general interpretation guidelines
Soil depth gradients of the δ13C signature of SOC can be used to identify vegetation changes and soil processes that affect C compounds. However, due to the simultaneous impact of multiple factors, their interpretation is often hampered and requires care. The interpretation must be specific to the pedo-climatic conditions and the soil type. To identify soil processes several major factors impacting δ13C signatures and the soil δ13C depth gradients need to be checked first in order to exclude them as drivers. In the following figure we summarize some steps that should be considered when analysing depth gradients of the δ13C signature of SOC and describe which additional data could be useful for an adequate interpretation (Fig. 6).
In the following, we want to give some examples of depth gradients of the δ13C signature of SOC for several soils to illustrate the soil type and site-specific context that always need to be accounted when interpreting such data. Histosols often show slightly decreasing or uniform δ13C signatures with depth, possibly because of the stabilisation of C under anaerobic, waterlogged conditions and the lower microbial fractionation (Krull and Retallack 2000; Alewell et al. 2011; Freeman et al. 2001). Anaerobic mineralisation could elevate the proportion of isotopically depleted organic substances such as lignin in the remaining C fraction without a kinetic isotope fractionation and preferential release of 12C, as observed during aerobic turnover (Benner et al. 1987; Alewell et al. 2011). This could be a driver for lower δ13C signatures in deep anoxic peat layers (Fig. 7). Environmental drivers, such as moisture availability (Xia et al. 2020) or the Suess effect on the isotopic signature of C assimilated by Sphagnum mosses and the depth of the groundwater table (100 cm), could explain the increase in the δ13C signature in the upper 100 cm of the illustrated example (Estop-Aragonés 2022). Furthermore, Gleysols show more negative δ13C signatures in groundwater-influenced horizons than other soils with aerobic conditions at similar depths, possibly due to the preservation of plant materials under anoxic conditions or the influence of allochthonous C from aquatic sources in the Holocenic parent material with depleted isotopic signatures (Schneider et al. 2021). Likewise, mineral arable soils with a mean groundwater table shallower than 80 cm show 1.4‰ lower δ13C signatures at 70–100 cm depth than soils with a mean groundwater table deeper than 200 cm (Schneider et al. 2021). Even in the topsoil of these soils where the groundwater does not affect the degradation of SOC, the δ13C signature of SOC is more depleted probably because plants are under water stress less often when groundwater tables are high, leading to more depleted isotopic signatures of C3 plants and SOC in the topsoil (Schneider et al. 2021; Boutton 1996).
Depth gradients of the ẟ13C signature of soil organic carbon and the radiocarbon age in a mature thermokarst bog in northern Alberta, Canada. The grey line shows the depth gradient of the ẟ13C signature and the black line shows the radiocarbon age in years before present (logarithmic scale). Data originate from Estop-Aragonés (2022)
Mature boreal forests show particularly steep depth gradients of ẟ13C signatures of about 3–4‰ from the organic to the underlying mineral soil horizons (Högberg et al. 2005). This can be explained by a combination of a high accumulation of fresh, depleted, plant-derived SOC and a high fungi/bacteria ratio in the topsoil and organic layer and the leaching of enriched hydrophilic DOC. The high depth gradient could be also promoted by a higher proportion of root-derived SOC in the subsoil than in the topsoil, if the δ13C signature of the roots of the woody plants is higher than that of the litter and of herbaceous plants at the soil surface (Philben et al. 2022).
There are also sites where δ13C signatures decrease with depth like in two Australian Vertisols (Krull and Skjemstad 2003). The authors explained the gradients by the stabilisation of depleted alkyl C in a low pH environment with clay-rich minerals. Such negative 13C trends could also be due to a temporally increasing proportion of C4 over C3 plant vegetation. In particular in agricultural topsoils, the isotopic δ13C signature is affected mostly by land use and C4 plants in crop rotation, with maize and other C4 cropping reducing the depth gradient of the δ13C signatures by elevating the topsoil δ13C signatures (Schneider et al. 2021). In general, it has been shown that the ẟ13C signature of SOC increases on average by 1.5‰ in German grasslands within 100 cm, and only by about 0.7‰ in croplands without maize cultivation (Fig. 8). The low depth gradient of croplands, which can be observed even without the cultivation of C4 plants, could be explained by a homogenising of the SOC with tillage in the plough layer. Tillage makes the impacts of the Suess effect and the effect of increasing pCO2 fuzzy, which are otherwise most dominant in the upper 30 cm of the soil profile. Another reason for the low depth gradients could be the low incorporation of fresh organic C compounds into the soil due to the removal of large biomass fractions with agricultural products (Smith et al. 2007).
Average depth gradients of ẟ13C signatures (solid lines) and organic carbon content (dashes lines) in German agricultural soils for croplands (orange) and grasslands (green), adapted from Schneider et al. (2021)
A comparison can be made between the depth gradients of the δ13C signature of SOC in archived pre-industrial soil cores and modern soil cores, allowing the exclusion of the effects of changing isotopic signatures of atmospheric CO2 on the plant-derived C inputs. Torn et al. (2002) found similar soil depth gradients of δ13C signatures increasing by 2‰ within one metre in an archived soil core (from about 1900) and a modern soil core from the same site in a Russian steppe. They observed that C stocks have not changed significantly over the last 100 years, and that the depth gradient of the δ13C signature was similar in both cores and could thus not be attributed to the Suess effect (Torn et al. 2002). It should be considered that the soil under a steppe vegetation stores high amounts of C and the small proportion of the newly formed C may have only a minor effect on the δ13C of bulk SOC. Thus, the results cannot be generalised to other sites. The study nevertheless shows that the trend in δ13C signature with depth also arises without the Suess effect and that other drivers must also be of importance. The isotopic enrichment with 13C within the pre-industrial soil core could be caused by changing δ13C signatures of plant C inputs through vegetation shifts and changes in the amount of precipitation or could be related to soil processes such as the accumulation of 13C-enriched bacterial necromass in the subsoil. Pollen records can be used (if existing) in combination with δ13C data to identify vegetation changes (Rao et al. 2012) and separate this driver from the other internal and external drivers and processes affecting the δ13C signature of SOC within soil profiles.
Conclusions and outlook
In general, the Suess effect, the effect of increasing atmospheric CO2 concentrations, the distribution pattern of microbial necromass (fungi/bacteria ratio) in the soil column, and the translocation of enriched hydrophilic DOC in the subsoil are ubiquitous factors that enhance the δ13C signatures of SOC with depth in most soil profiles. Other factors are time-dependent, such as fractionation during decomposition stages, or site-specific, such as the effects of changing precipitation and vegetation composition or the contribution of geogenic and allochthonous C to SOC. Vegetation changes can dominate the depth gradients of δ13C signatures when C4 plants replace C3 plants or vice versa (about ± 14‰). This can be much higher than the Suess effect (about 2.0‰), the effect of increasing atmospheric CO2 (about 1.8‰) or environmental effects on the photosynthetic 13C fractionation (up to ± 3‰). Likewise, the variation in the proportion of enriched necromass to SOC and its fungi/bacteria ratio as well as the leaching of enriched DOC can lead to additional increases in the isotopic signature of up to 3‰. Aspects such as preferential decomposition, lower δ13C signatures of atmospheric CO2 in the early Holocene relative to the preindustrial signature, and the effects of drier or more nutritious conditions on plant photosynthesis are in contrast with the commonly observed enrichment of 13C with soil depth. However, these are of relatively subordinate importance in most soil profiles. External drivers, such as changes in atmospheric and climate conditions, are increasing in their importance and seem to be stronger in shaping the ẟ13C depth gradients than internal soil processes.
Future interpretations of depth gradients of δ13C signatures of SOC regarding internal soil processes should be aligned with pollen records to ascertain or rule out the impacts of vegetation changes. The storage, translocation and isotopic signature of pyrogenic C, PhytOC, DOC and the necromass of several microbes (fungi, bacteria, archaea) should be further studied to improve modelling of C cycles and elucidate their importance in SOC stabilisation. The effects of roots on the amount of SOC and the δ13C signature in the subsoil should be further evaluated in several recent ecosystems and on larger timescales (during whole pedogenesis). Future research should assess how fresh DOC from deep roots can cause priming effects on native SOC (Schiedung et al. 2023), potentially resulting in changes in the δ13C and Δ14C signatures of the SOC. The cumulative small effects of the C recycling of different microbial communities on the isotopic signatures of SOC deserve further attention. New experiments should be designed to consider the internal and external drivers and processes affecting the δ13C signatures of SOC separately.
References
Abraham W-R, Hesse C (2003) Isotope fractionations in the biosynthesis of cell components by different fungi: a basis for environmental carbon flux studies. FEMS Microbiol Ecol 46:121–128. https://doi.org/10.1016/S0168-6496(03)00203-4
Alewell C, Giesler R, Klaminder J, Leifeld J, Rollog M (2011) Stable carbon isotopes as indicators for environmental change in palsa peats. Biogeosciences 8:1769–1778. https://doi.org/10.5194/bg-8-1769-2011
Amiotte-Suchet P, Probst J-L, Ludwig W (2003) Worldwide distribution of continental rock lithology: implications for the atmospheric/soil CO2 uptake by continental weathering and alkalinity river transport to the oceans. Glob Biogeochem Cycles 17. https://doi.org/10.1029/2002GB001891
Angle JC, Morin TH, Solden LM, Narrowe AB, Smith GJ, Borton MA, Rey-Sanchez C, Daly RA, Mirfenderesgi G, Hoyt DW, Riley WJ, Miller CS, Bohrer G, Wrighton KC (2017) Methanogenesis in oxygenated soils is a substantial fraction of wetland methane emissions. Nat Commun 8:1567. https://doi.org/10.1038/s41467-017-01753-4
Balesdent J, Basile-Doelsch I, Chadoeuf J, Cornu S, Derrien D, Fekiacova Z, Hatté C (2018a) Atmosphere-soil carbon transfer as a function of soil depth. Nature 559:599–602. https://doi.org/10.1038/s41586-018-0328-3
Balesdent J, Basile-Doelsch I, Chadoeuf J, Cornu S, Derrien D, Fekiacova Z, Hatté C (2018b) Depth distribution of soil carbon age inferred from natural 13 C labelling meta-analysis. Recherche Data Gouv, V1. https://doi.org/10.15454/KMNR6R
Balesdent J, Mariotti A (1996) Measurement of soil organic matter turnover using 13C natural abundance. In: Boutton TW, Yamasaki S (eds) Mass spectrometry of soils. M. Dekker, New York, pp 83–111
Barker JF, Fritz P (1981) Carbon isotope fractionation during microbial methane oxidation. Nature 293:289–291. https://doi.org/10.1038/293289a0
Bashkin MA, Binkley D (1998) Changes in soil carbon following afforestation in Hawaii. Ecology 79:828. https://doi.org/10.2307/176582
Basile-Doelsch I, Brun T, Borschneck D, Masion A, Marol C, Balesdent J (2009) Effect of landuse on organic matter stabilized in organomineral complexes: a study combining density fractionation, mineralogy and δ13C. Geoderma 151:77–86. https://doi.org/10.1016/j.geoderma.2009.03.008
Basu S, Agrawal S, Sanyal P, Mahato P, Kumar S, Sarkar A (2015) Carbon isotopic ratios of modern C3–C4 plants from the Gangetic Plain, India and its implications to paleovegetational reconstruction. Palaeogeogr Palaeoclimatol Palaeoecol 440:22–32. https://doi.org/10.1016/j.palaeo.2015.08.012
Beer C, Reichstein M, Tomelleri E, Ciais P, Jung M, Carvalhais N, Rödenbeck C, Arain MA, Baldocchi D, Bonan GB, Bondeau A, Cescatti A, Lasslop G, Lindroth A, Lomas M, Luyssaert S, Margolis H, Oleson KW, Roupsard O, Veenendaal E, Viovy N, Williams C, Woodward FI, Papale D (2010) Terrestrial gross carbon dioxide uptake: global distribution and covariation with climate. Science 329:834–838. https://doi.org/10.1126/science.1184984
Benner R, Fogel ML, Sprague EK, Hodson RE (1987) Depletion of 13 C in lignin and its implications for stable carbon isotope studies. Nature 329:708–710. https://doi.org/10.1038/329708a0
Bird MI, Ascough PL (2012) Isotopes in pyrogenic carbon: a review. Org Geochem 42:1529–1539. https://doi.org/10.1016/j.orggeochem.2010.09.005
Bird M, Kracht O, Derrien D, Zhou Y (2003) The effect of soil texture and roots on the stable carbon isotope composition of soil organic carbon. Soil Res 41:77. https://doi.org/10.1071/SR02044
Bird MI, Pousai P (1997) Variations of δ13C in the surface soil organic carbon pool. Glob Biogeochem Cycles 11:313–322. https://doi.org/10.1029/97GB01197
Blagodatskaya Е, Kuzyakov Y (2008) Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure: critical review. Biol Fertil Soils 45:115–131. https://doi.org/10.1007/s00374-008-0334-y
Bloom AJ, Troughton JH (1979) High productivity and photosynthetic flexibility in a CAM plant. Oecologia 38:35–43. https://doi.org/10.1007/BF00347822
Bol R, Eriksen J, Smith P, Garnett MH, Coleman K, Christensen BT (2005) The natural abundance of 13 C, 15 N, 34S and 14 C in archived (1923–2000) plant and soil samples from the Askov long-term experiments on animal manure and mineral fertilizer. Rapid Commun Mass Spectrom 19:3216–3226. https://doi.org/10.1002/rcm.2156
Boström B, Comstedt D, Ekblad A (2007) Isotope fractionation and 13 C enrichment in soil profiles during the decomposition of soil organic matter. Oecologia 153:89–98. https://doi.org/10.1007/s00442-007-0700-8
Boutton TW (1996) Stable carbon isotope ratios of soil organic matter and their use as indicators of vegetation and climate change. In: Boutton TW, Yamasaki S (eds) Mass spectrometry of soils. M. Dekker, New York, pp 47–82
Boutton TW, Archer SR, Midwood AJ, Zitzer SF, Bol R (1998) δ13C values of soil organic carbon and their use in documenting vegetation change in a subtropical savanna ecosystem. Geoderma 82:5–41. https://doi.org/10.1016/S0016-7061(97)00095-5
Boutton TW, Nordt LC, Archer SR, Midwood AJ, Casar I (1993) Stable carbon isotope ratios of soil organic matter and their potential use as indicators of palaeoclimate. In: IAEA (ed), Isotope techniques in the study of past and current environmental changes in the hydrosphere and the atmosphere. Proceedings of an international symposium on applications of isotope techniques in studying past and current environmental changes in the hydrosphere and the atmosphere, held in Vienna, 19–23 April 1993, Vol 908. IAEA, Vienna
Bowling DR, Pataki DE, Randerson JT (2008) Carbon isotopes in terrestrial ecosystem pools and CO2 fluxes. New Phytol 178:24–40. https://doi.org/10.1111/j.1469-8137.2007.02342.x
Braconnot P, Otto-Bliesner B, Harrison S, Joussaume S, Peterchmitt J-Y, Abe-Ouchi A, Crucifix M, Driesschaert E, Fichefet T, Hewitt CD, Kageyama M, Kitoh A, Laîné A, Loutre M-F, Marti O, Merkel U, Ramstein G, Valdes P, Weber SL, Yu Y, Zhao Y (2007) Results of PMIP2 coupled simulations of the mid-holocene and last glacial maximum – part 1: experiments and large-scale features. Clim Past 3:261–277. https://doi.org/10.5194/cp-3-261-2007
Briones M, Ineson P, Sleep D (1999) Use of δ13C to determine food selection in collembolan species. Soil Biol Biochem 31:937–940. https://doi.org/10.1016/S0038-0717(98)00179-5
Buchmann N, Kao W-Y, Ehleringer J (1997) Influence of stand structure on carbon-13 of vegetation, soils, and canopy air within deciduous and evergreen forests in Utah, United States. Oecologia 110:109–119. https://doi.org/10.1007/s004420050139
Canadell J, Jackson RB, Ehleringer JB, Mooney HA, Sala OE, Schulze E-D (1996) Maximum rooting depth of vegetation types at the global scale. Oecologia 108:583–595. https://doi.org/10.1007/BF00329030
Canadell JG, Monteiro P, Costa MH, Cotrim da Cunha L, Cox PM, Eliseev AV, Henson S, Ishii M, Jaccard S, Koven C, Lohila A, Patra PK, Piao S, Rogelj J, Syampungani S, Zaehle S, Zickfeld K (2021) Global Carbon and other Biogeochemical Cycles and Feedbacks. IPCC, Chap 5. In: Masson-Delmotte V, Zhai P, Pirani A, Connors SL, Péan C, Berger S, Caud N, Chen Y, Goldfarb L, Gomis MI, Huang M, Leitzell K, Lonnoy E, Matthews J, Maycock TK, Waterfield T, Yelekçi O, Yu R, Zhou B (eds) Climate change 2021: the physical science basis. contribution of working group I to the sixth assessment report of the intergovernmental panel on climate change. Cambridge University Press, pp 673–816. https://doi.org/10.1017/9781009157896.007
Cerling TE, Solomon D, Quade J, Bowman JR (1991) On the isotopic composition of carbon in soil carbon dioxide. Geochim Cosmochim Acta 55:3403–3405. https://doi.org/10.1016/0016-7037(91)90498-T
Cernusak LA, Tcherkez G, Keitel C, Cornwell WK, Santiago LS, Knohl A, Barbour MM, Williams DG, Reich PB, Ellsworth DS, Dawson TE, Griffiths HG, Farquhar GD, Wright IJ (2009) Why are non-photosynthetic tissues generally 13 C enriched compared with leaves in C3 plants? Review and synthesis of current hypotheses. Funct Plant Biol: FPB 36:199–213. https://doi.org/10.1071/FP08216
Conrad R (1999) Contribution of hydrogen to methane production and control of hydrogen concentrations in methanogenic soils and sediments. FEMS Microbiol Ecol 28:193–202. https://doi.org/10.1016/S0168-6496(98)00086-5
Dalal RC (1998) Soil microbial biomass—what do the numbers really mean? Aust J Exp Agric 38:649. https://doi.org/10.1071/ea97142
Dalal RC, Harms BP, Krull E, Wang WJ (2005) Total soil organic matter and its labile pools following mulga (Acacia aneura) clearing for pasture development and cropping 1. Total and labile carbon. Soil Res 43:13. https://doi.org/10.1071/SR04044
Deines P (1988) The isotopic composition of reduced organic carbon. In: Fritz P (ed) Handbook of environmental isotope geochemistry, 1st edn. Elsevier, Amsterdam, pp 329–406
Delgado-Baquerizo M, Oliverio AM, Brewer TE, Benavent-González A, Eldridge DJ, Bardgett RD, Maestre FT, Singh BK, Fierer N (2018) A global atlas of the dominant bacteria found in soil. Science 359:320–325. https://doi.org/10.1126/science.aap9516
Desjardins T, Volkoff B, Andreux F, Cerri C (1991) Distribution du carbone total et de l’isotope 13 C dans des sols ferrallitiques du Brésil. Sci du Sol 29:175–187
Dijkstra P, Ishizu A, Doucett R, Hart SC, Schwartz E, Menyailo OV, Hungate BA (2006) 13 C and 15 N natural abundance of the soil microbial biomass. Soil Biol Biochem 38:3257–3266. https://doi.org/10.1016/j.soilbio.2006.04.005
Diochon A, Kellman L (2008) Natural abundance measurements of 13 C indicate increased deep soil carbon mineralization after forest disturbance. Geophys Res Lett 35:L14402. https://doi.org/10.1029/2008GL034795
Dorodnikov M, Knorr K-H, Fan L, Kuzyakov Y, Nilsson MB (2022) A novel belowground in-situ gas labeling approach: CH4 oxidation in deep peat using passive diffusion chambers and 13 C excess. Sci Total Environ 806:150457. https://doi.org/10.1016/j.scitotenv.2021.150457
Dungait JAJ, Hopkins DW, Gregory AS, Whitmore AP (2012) Soil organic matter turnover is governed by accessibility not recalcitrance. Glob Change Biol 18:1781–1796. https://doi.org/10.1111/j.1365-2486.2012.02665.x
Eggleston S, Schmitt J, Bereiter B, Schneider R, Fischer H (2016) Evolution of the stable carbon isotope composition of atmospheric CO2 over the last glacial cycle. Paleoceanography 31:434–452. https://doi.org/10.1002/2015PA002874
Ehleringer JR, Buchmann N, Flanagan LB (2000) Carbon isotope ratios in belowground carbon cycle processes. Ecol Appl 412–422. https://doi.org/10.2307/2641103
Ehleringer JR, Cerling TE (2002) C3 and C4 photosynthesis. In: Mooney HA, Canadell JG, Munn RE (eds) Encyclopedia of global environmental change. The earth system: biological and ecological dimensions of global environmental change. Wiley, Chichester, pp 186–190
Elsig J, Schmitt J, Leuenberger D, Schneider R, Eyer M, Leuenberger M, Joos F, Fischer H, Stocker TF (2009) Stable isotope constraints on Holocene carbon cycle changes from an Antarctic ice core. Nature 461:507–510. https://doi.org/10.1038/nature08393
Estop-Aragonés C (2022) Anoxic incubation and priming experiment in a permafrost and thermokarst peatland in Lutose, Alberta, Canada. Zenodo. https://doi.org/10.5281/zenodo.6326854
Farquhar GD (1991) Nuclear techniques in soil-plant studies for sustainable agriculture and environmental preservation. Proceedings of an internat. symp. on nuclear and related techniques in soil-plant studies on sustain. agric. and environm. preservation jointly organ. by the International Atomic Energy Agency and the Food and Agricultural Organization if the United Nations and held in Vienna 17–21 Oct. 1994. IAEA, International Atomic Energy Agency (IAEA)
Farquhar GD, Ehleringer JR, Hubick KT (1989) Carbon Isotope discrimination and photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 40:503–537. https://doi.org/10.1146/annurev.pp.40.060189.002443
Farquhar GD, O’Leary MH, Berry JA (1982) On the relationship between carbon isotope discrimination and the intercellular carbon dioxide concentration in leaves. Funct Plant Biol 9:121. https://doi.org/10.1071/pp9820121
Feng X (2002) A theoretical analysis of carbon isotope evolution of decomposing plant litters and soil organic matter. Global Biogeochem Cycles 16:66–1–66–11. https://doi.org/10.1029/2002GB001867
Fernandez I, Mahieu N, Cadisch G (2003) Carbon isotopic fractionation during decomposition of plant materials of different quality. Global Biogeochem Cycles 17. https://doi.org/10.1029/2001GB001834
Fontaine S, Barot S, Barré P, Bdioui N, Mary B, Rumpel C (2007) Stability of organic carbon in deep soil layers controlled by fresh carbon supply. Nature 450:277–280. https://doi.org/10.1038/nature06275
Freeman C, Ostle N, Kang H (2001) An enzymic ‘latch’ on a global carbon store. Nat: Int Wkly J Sci 409:149. https://doi.org/10.1038/35051650
Friedlingstein P, O’Sullivan M, Jones MW, Andrew RM, Hauck J, Olsen A, Peters GP, Peters W, Pongratz J, Sitch S, Le Quéré C, Canadell JG, Ciais P, Jackson RB, Alin S, Aragão LEOC, Arneth A, Arora V, Bates NR, Becker M, Benoit-Cattin A, Bittig HC, Bopp L, Bultan S, Chandra N, Chevallier F, Chini LP, Evans W, Florentie L, Forster PM, Gasser T, Gehlen M, Gilfillan D, Gkritzalis T, Gregor L, Gruber N, Harris I, Hartung K, Haverd V, Houghton RA, Ilyina T, Jain AK, Joetzjer E, Kadono K, Kato E, Kitidis V, Korsbakken JI, Landschützer P, Lefèvre N, Lenton A, Lienert S, Liu Z, Lombardozzi D, Marland G, Metzl N, Munro DR, Nabel JEMS, Nakaoka S-I, Niwa Y, O’Brien K, Ono T, Palmer PI, Pierrot D, Poulter B, Resplandy L, Robertson E, Rödenbeck C, Schwinger J, Séférian R, Skjelvan I, Smith AJP, Sutton AJ, Tanhua T, Tans PP, Tian H, Tilbrook B, van der Werf G, Vuichard N, Walker AP, Wanninkhof R, Watson AJ, Willis D, Wiltshire AJ, Yuan W, Yue X, Zaehle S (2020) Global carbon budget 2020. Earth Syst Sci Data 12:3269–3340. https://doi.org/10.5194/essd-12-3269-2020
Fu QA, Boutton TW, Ehleringer JR, Flagler RB (1993) 20 - environmental and developmental effects on carbon isotope discrimination by two species of Phaseolus. In: Ehleringer JR, Hall AE, Farquhar DF (eds) Stable isotopes and plant carbon-water relations. Academic, San Diego, pp 297–309
Gamage D, Thompson M, Sutherland M, Hirotsu N, Makino A, Seneweera S (2018) New insights into the cellular mechanisms of plant growth at elevated atmospheric carbon dioxide concentrations. Plant Cell Environ 41:1233–1246. https://doi.org/10.1111/pce.13206
Gerzabek MH, Pichlmayer F, Kirchmann H, Haberhauer G (1997) The response of soil organic matter to manure amendments in a long-term experiment at Ultuna, Sweden. Eur J Soil Sci 48:273–282. https://doi.org/10.1111/j.1365-2389.1997.tb00547.x
Ghashghaie J, Badeck FW (2014) Opposite carbon isotope discrimination during dark respiration in leaves versus roots - a review. New Phytol 201:751–769. https://doi.org/10.1111/nph.12563
Gleixner G, Danier HJ, Werner RA, Schmidt HL (1993) Correlations between the 13 C content of primary and secondary Plant Products in different cell compartments and that in decomposing Basidiomycetes. Plant Physiol 102:1287–1290. https://doi.org/10.1104/pp.102.4.1287
Godbold DL, Hoosbeek MR, Lukac M, Cotrufo MF, Janssens IA, Ceulemans R, Polle A, Velthorst EJ, Scarascia-Mugnozza G, de Angelis P, Miglietta F, Peressotti A (2006) Mycorrhizal hyphal turnover as a dominant process for carbon input into soil organic matter. Plant Soil 281:15–24. https://doi.org/10.1007/s11104-005-3701-6
Graven H, Allison CE, Etheridge DM, Hammer S, Keeling RF, Levin I, Meijer HAJ, Rubino M, Tans PP, Trudinger CM, Vaughn BH, White JWC (2017) Compiled records of carbon isotopes in atmospheric CO2 for historical simulations in CMIP6. Geosci Model Dev 10:4405–4417. https://doi.org/10.5194/gmd-10-4405-2017
Graven H, Keeling RF, Rogelj J (2020) Changes to carbon isotopes in atmospheric CO2 over the industrial era and into the future. Global Biogeochem Cycles 34. https://doi.org/10.1029/2019GB006170
Guillet B, Achoundong G, Happi JY, Beyala V, Kamgang K, Bonvallot J, Riera B, Mariotti A, Schwartz D (2001) Agreement between floristic and soil organic carbon isotope (13 C/ 12 C, 14 C) indicators of forest invasion of savannas during the last century in Cameroon. J Trop Ecol 17:809–832. https://doi.org/10.1017/S0266467401001614
Hare VJ, Loftus E, Jeffrey A, Ramsey CB (2018) Atmospheric CO2 effect on stable carbon isotope composition of terrestrial fossil archives. Nat Commun 9:252. https://doi.org/10.1038/s41467-017-02691-x
Heim A, Schmidt MWI (2007) Lignin turnover in arable soil and grassland analysed with two different labelling approaches. Eur J Soil Sci 58:599–608. https://doi.org/10.1111/j.1365-2389.2006.00848.x
Henneron L, Balesdent J, Alvarez G, Barré P, Baudin F, Basile-Doelsch I, Cécillon L, Fernandez-Martinez A, Hatté C, Fontaine S (2022) Bioenergetic control of soil carbon dynamics across depth. Nat Commun 13:7676. https://doi.org/10.1038/s41467-022-34951-w
Herzschuh U, Böhmer T, Chevalier M, Dallmeyer A, Li C, Cao X, Hébert R, Peyron O, Nazarova L, Novenko EY, Park J, Rudaya NA, Schlütz F, Shumilovskikh LS, Tarasov PE, Wang Y, Wen R, Xu Q, Zheng Z (2022) Regional pollen-based Holocene temperature and precipitation patterns depart from the Northern Hemisphere mean trends. EGUsphere:1–23. https://doi.org/10.5194/egusphere-2022-127
Hicks Pries CE, Sulman BN, West C, O’Neill C, Poppleton E, Porras RC, Castanha C, Zhu B, Wiedemeier DB, Torn MS (2018) Root litter decomposition slows with soil depth. Soil Biol Biochem 125:103–114. https://doi.org/10.1016/j.soilbio.2018.07.002
Hindshaw RS, Lang SQ, Bernasconi SM, Heaton THE, Lindsay MR, Boyd ES (2016) Origin and temporal variability of unusually low δ13 C-DOC values in two High Arctic catchments. Journal of Geophysical Research: Biogeosciences 121:1073–1085. https://doi.org/10.1002/2015JG003303
Ho A, Lee HJ, Reumer M, Meima-Franke M, Raaijmakers C, Zweers H, de Boer W, van der Putten WH, Bodelier PL (2019) Unexpected role of canonical aerobic methanotrophs in upland agricultural soils. Soil Biol Biochem 131:1–8. https://doi.org/10.1016/j.soilbio.2018.12.020
Hobbie EA, Weber NS, Trappe JM (2001) Mycorrhizal vs saprotrophic status of fungi: the isotopic evidence. New Phytol 150:601–610. https://doi.org/10.1046/j.1469-8137.2001.00134.x
Högberg P, Ekblad A, Nordgren A, Plamboeck AH, Ohlsson A, Bhupinderpal-Singh, Högberg MN (2005) 4 - Factors Determining the 13C Abundance of Soil-Respired CO2 in Boreal Forests. In: Flanagan LB, Ehleringer JR, Pataki DE (eds), Stable Isotopes and Biosphere Atmosphere Interactions. Academic, San Diego, pp 47–68
Högberg P, Plamboeck AH, Taylor AF, Fransson PM (1999) Natural (13)C abundance reveals trophic status of fungi and host-origin of carbon in mycorrhizal fungi in mixed forests. Proc Natl Acad Sci USA 96:8534–8539. https://doi.org/10.1073/pnas.96.15.8534
Iversen CM, Keller JK, Garten CT, Norby RJ (2012) Soil carbon and nitrogen cycling and storage throughout the soil profile in a sweetgum plantation after 11 years of CO2-enrichment. Glob Chang Biol 18:1684–1697. https://doi.org/10.1111/j.1365-2486.2012.02643.x
Jackson RB, Lajtha K, Crow SE, Hugelius G, Kramer MG, Piñeiro G (2017) The Ecology of Soil Carbon: Pools, Vulnerabilities, and Biotic and Abiotic Controls. Annu Rev Ecol Evol Syst 48:419–445. https://doi.org/10.1146/annurev-ecolsys-112414-054234
Ji M, Greening C, Vanwonterghem I, Carere CR, Bay SK, Steen JA, Montgomery K, Lines T, Beardall J, van Dorst J, Snape I, Stott MB, Hugenholtz P, Ferrari BC (2017) Atmospheric trace gases support primary production in Antarctic desert surface soil. Nature 552:400–403. https://doi.org/10.1038/nature25014
Jones MW, Santín C, van der Werf GR, Doerr SH (2019) Global fire emissions buffered by the production of pyrogenic carbon. Nat Geosci 12:742–747. https://doi.org/10.1038/s41561-019-0403-x
Joos F, Gerber S, Prentice IC, Otto-Bliesner BL, Valdes PJ (2004) Transient simulations of Holocene atmospheric carbon dioxide and terrestrial carbon since the Last Glacial Maximum. Global biogeochemical cycles 18. https://doi.org/10.1029/2003GB002156
Kaiser K, Guggenberger G, Zech W (2001) Isotopic fractionation of dissolved organic carbon in shallow forest soils as affected by sorption. Eur J Soil Sci 52:585–597. https://doi.org/10.1046/j.1365-2389.2001.00407.x
Kaiser K, Kalbitz K (2012) Cycling downwards – dissolved organic matter in soils. Soil Biol Biochem 52:29–32. https://doi.org/10.1016/j.soilbio.2012.04.002
Kalbitz K, Kaiser K, Bargholz J, Dardenne P (2006) Lignin degradation controls the production of dissolved organic matter in decomposing foliar litter. Eur J Soil Sci 57:504–516. https://doi.org/10.1111/j.1365-2389.2006.00797.x
Kalks F, Noren G, Mueller CW, Helfrich M, Rethemeyer J, Don A (2021) Geogenic organic carbon in terrestrial sediments and its contribution to total soil carbon. SOIL 7:347–362. https://doi.org/10.5194/soil-7-347-2021
Kaplan JO, Prentice IC, Buchmann N (2002) The stable carbon isotope composition of the terrestrial biosphere: modeling at scales from the leaf to the globe. Glob Biogeochem Cycles 16:8-1-8–11
Kästner M, Miltner A, Thiele-Bruhn S, Liang C (2021) Microbial Necromass in Soils—Linking microbes to soil processes and Carbon turnover. Front Environ Sci 9. https://doi.org/10.3389/fenvs.2021.756378
Keeling CD, Piper SC, Bacastow RB, Wahlen M, Whorf TP, Heimann M, Meijer HA (2001) Exchanges of atmospheric CO2 and 13CO2 with the terrestrial biosphere and oceans from 1978 to 2000. I. Global Aspects. Scripps Institution of Oceanography, US San Diego, San Diego
Keeling RF, Graven HD, Welp LR, Resplandy L, Bi J, Piper SC, Sun Y, Bollenbacher A, Meijer HAJ (2017) Atmospheric evidence for a global secular increase in carbon isotopic discrimination of land photosynthesis. Proc Natl Acad Sci 114:10361–10366. https://doi.org/10.1073/pnas.1619240114
Khanongnuch R, Mangayil R, Santala V, Hestnes AG, Svenning MM, Rissanen AJ (2022) Batch experiments demonstrating a two-stage bacterial process coupling methanotrophic and heterotrophic bacteria for 1-Alkene production from methane. Front Microbiol. https://doi.org/10.3389/fmicb.2022.874627
Kirschbaum MU, Don A, Beare MH, Hedley MJ, Pereira RC, Curtin D, McNally SR, Lawrence-Smith EJ (2021) Sequestration of soil carbon by burying it deeper within the profile: a theoretical exploration of three possible mechanisms. Soil Biol Biochem 163:108432. https://doi.org/10.1016/j.soilbio.2021.108432
Klink S, Keller AB, Wild AJ, Baumert VL, Gube M, Lehndorff E, Meyer N, Mueller CW, Phillips RP, Pausch J (2022) Stable isotopes reveal that fungal residues contribute more to mineral-associated organic matter pools than plant residues. Soil Biol Biochem 168:108634. https://doi.org/10.1016/j.soilbio.2022.108634
Kohl L, Laganière J, Edwards KA, Billings SA, Morrill PL, van Biesen G, Ziegler SE (2015) Distinct fungal and bacterial δ13C signatures as potential drivers of increasing δ13C of soil organic matter with depth. Biogeochemistry 124:13–26. https://doi.org/10.1007/s10533-015-0107-2
Kohn MJ (2010) Carbon isotope compositions of terrestrial C3 plants as indicators of (paleo)ecology and (paleo)climate. Proc Natl Acad Sci USA 107:19691–19695. https://doi.org/10.1073/pnas.1004933107
Kopittke PM, Hernandez-Soriano MC, Dalal RC, Finn D, Menzies NW, Hoeschen C, Mueller CW (2018) Nitrogen-rich microbial products provide new organo-mineral associations for the stabilization of soil organic matter. Glob Change Biol 24:1762–1770. https://doi.org/10.1111/gcb.14009
Kristiansen SM, Brandt M, Hansen EM, Magid J, Christensen BT (2004) 13 C signature of CO2 evolved from incubated maize residues and maize-derived sheep faeces. Soil Biol Biochem 36:99–105. https://doi.org/10.1016/j.soilbio.2003.07.002
Krull ES, Retallack GJ (2000) δ13C depth profiles from paleosols across the Permian-Triassic boundary: evidence for methane release. GSA Bull 112:1459–1472. https://doi.org/10.1130/0016-7606(2000)112<1459:CDPFPA>2.0.CO;2
Krull ES, Skjemstad JO (2003) δ13C and δ15N profiles in 14 C-dated Oxisol and Vertisols as a function of soil chemistry and mineralogy. Geoderma 112:1–29. https://doi.org/10.1016/S0016-7061(02)00291-4
Krull ES, Skjemstad JO, Burrows WH, Bray SG, Wynn JG, Bol R, Spouncer L, Harms B (2005) Recent vegetation changes in central Queensland, Australia: evidence from δ13C and 14 C analyses of soil organic matter. Geoderma 126:241–259. https://doi.org/10.1016/j.geoderma.2004.09.012
Kuyper TW (2016) Chap. 20 - Carbon and energy sources of mycorrhizal fungi: obligate symbionts or latent saprotrophs? In: Johnson NC, Gehring C, Jansa J (eds) Mycorrhizal mediation of soil. Fertility, structure, and carbon storage. Elsevier, Amsterdam, pp 357-374
Lansdown J, Quay P, King S (1992) CH4 production via CO2 reduction in a temperate bog: a source of 13 C-depIeted CH4. Geochim Cosmochim Acta 56:3493–3503. https://doi.org/10.1016/0016-7037(92)90393-W
Le Mer J, Roger P (2001) Production, oxidation, emission and consumption of methane by soils: a review. Eur J Soil Biol 37:25–50. https://doi.org/10.1016/S1164-5563(01)01067-6
Liang C, Schimel JP, Jastrow JD (2017) The importance of anabolism in microbial control over soil carbon storage. Nat Microbiol 2:17105. https://doi.org/10.1038/nmicrobiol.2017.105
Liang C, Amelung W, Lehmann J, Kästner M (2019) Quantitative assessment of microbial necromass contribution to soil organic matter. Glob Change Biol 25:3578–3590. https://doi.org/10.1111/gcb.14781
Lorenz K, Lal R, Preston CM, Nierop KG (2007) Strengthening the soil organic carbon pool by increasing contributions from recalcitrant aliphatic bio(macro)molecules. Geoderma 142:1–10. https://doi.org/10.1016/j.geoderma.2007.07.013
Lützow Mv, Kögel-Knabner I, Ekschmitt K, Matzner E, Guggenberger G, Marschner B, Flessa H (2006) Stabilization of organic matter in temperate soils: mechanisms and their relevance under different soil conditions - a review. Eur J Soil Sci 57:426–445. https://doi.org/10.1111/j.1365-2389.2006.00809.x
Ma J-Y, Sun W, Liu X-N, Chen F-H (2012) Variation in the stable carbon and nitrogen isotope composition of plants and soil along a precipitation gradient in northern China. PLoS ONE 7:e51894. https://doi.org/10.1371/journal.pone.0051894
Madhavan S, Treichel I, O’Leary MH (1991) Effects of relative humidity on carbon isotope fractionation in plants. Bot Acta 104:292–294. https://doi.org/10.1111/j.1438-8677.1991.tb00232.x
Marino BD, McElroy MB, Salawitch RJ, Spaulding WG (1992) Glacial-to-interglacial variations in the carbon isotopic composition of atmospheric CO2. Nature 357:461–466. https://doi.org/10.1038/357461a0
Mariotti A, Peterschmitt E (1994) Forest savanna ecotone dynamics in India as revealed by carbon isotope ratios of soil organic matter. Oecologia 97:475–480. https://doi.org/10.1007/BF00325885
Marschner B, Brodowski S, Dreves A, Gleixner G, Gude A, Grootes PM, Hamer U, Heim A, Jandl G, Ji R, Kaiser K, Kalbitz K, Kramer C, Leinweber P, Rethemeyer J, Schäffer A, Schmidt MWI, Schwark L, Wiesenberg GLB (2008) How relevant is recalcitrance for the stabilization of organic matter in soils? J Plant Nutr Soil Sci 171:91–110. https://doi.org/10.1002/jpln.200700049
Martinelli IA, Pessenda LCR, Espinoza E, Camargo PB, Telles FC, Cerri CC, Victoria RL, Aravena R, Richey J, Trumbore S (1996) Carbon-13 variation with depth in soils of Brazil and climate change during the Quaternary. Oecologia 106:376–381. https://doi.org/10.1007/BF00334565
Mathieu JA, Hatté C, Balesdent J, Parent É (2015) Deep soil carbon dynamics are driven more by soil type than by climate: a worldwide meta-analysis of radiocarbon profiles. Glob Change Biol 21:4278–4292. https://doi.org/10.1111/gcb.13012
Meija J, Coplen TB, Berglund M, Brand WA, de Bièvre P, Gröning M, Holden NE, Irrgeher J, Loss RD, Walczyk T, Prohaska T (2016) Isotopic compositions of the elements 2013. (IUPAC Technical Report). Pure Appl Chem 88:293–306. https://doi.org/10.1515/pac-2015-0503
Menichetti L, Houot S, van Oort F, Kätterer T, Christensen BT, Chenu C, Barré P, Vasilyeva NA, Ekblad A (2015) Increase in soil stable carbon isotope ratio relates to loss of organic carbon: results from five long-term bare fallow experiments. Oecologia 177:811–821. https://doi.org/10.1007/s00442-014-3114-4
Metya A, Chakraborty S, Bhattacharya SK, Datye A, Deb Burman PK, Dasgupta P, Sarma D, Gogoi N, Bora A (2021) Isotopic and concentration analyses of CO2 and CH4 in Association with the Eddy-Covariance based measurements in a Tropical Forest of Northeast India. Earth Space Sci 8:e2020EA001504
Mook WG, Bommerson JC, Staverman WH (1974) Carbon isotope fractionation between dissolved bicarbonate and gaseous carbon dioxide. Earth Planet Sci Lett 22:169–176. https://doi.org/10.1016/0012-821X(74)90078-8
Nel JA, Cramer MD (2019) Soil microbial anaplerotic CO2 fixation in temperate soils. Geoderma 335:170–178. https://doi.org/10.1016/j.geoderma.2018.08.014
Ni X, Liao S, Tan S, Peng Y, Wang D, Yue K, Wu F, Yang Y (2020) The vertical distribution and control of microbial necromass carbon in forest soils. Glob Ecol Biogeogr 29:1829–1839. https://doi.org/10.1111/geb.13159
O’Leary M (1988) Carbon isotopes in photosynthesis fractionation techniques may reveal new aspects of carbon dynamics in plants. BioScience
Osborne CP, Sack L (2012) Evolution of C4 plants: a new hypothesis for an interaction of CO2 and water relations mediated by plant hydraulics. Philos Trans R Soc Lond B Biol Sci 367:583–600. https://doi.org/10.1098/rstb.2011.0261
Osono T (2007) Ecology of ligninolytic fungi associated with leaf litter decomposition. Ecol Res 22:955–974. https://doi.org/10.1007/s11284-007-0390-z
Parr JF, Sullivan LA (2005) Soil carbon sequestration in phytoliths. Soil Biol Biochem 37:117–124. https://doi.org/10.1016/j.soilbio.2004.06.013
Penger J, Conrad R, Blaser M (2012) Stable carbon isotope fractionation by methylotrophic methanogenic archaea. Appl Environ Microbiol 78:7596–7602. https://doi.org/10.1128/AEM.01773-12
Pessenda LCR, Gouveia SEM, Ribeiro AS, de Oliveira PE, Aravena R (2010) Late pleistocene and holocene vegetation changes in northeastern Brazil determined from carbon isotopes and charcoal records in soils. Palaeogeogr Palaeoclimatol Palaeoecol 297:597–608. https://doi.org/10.1016/j.palaeo.2010.09.008
Philben M, Bowering K, Podrebarac FA, Laganière J, Edwards K, Ziegler SE (2022) Enrichment of 13 C with depth in soil organic horizons is not explained by CO2 or DOC losses during decomposition. Geoderma 424:116004. https://doi.org/10.1016/j.geoderma.2022.116004
Potthoff M, Loftfield N, Buegger F, Wick B, John B, Joergensen RG, Flessa H (2003) The determination of δ13C in soil microbial biomass using fumigation-extraction. Soil Biol Biochem 35:947–954. https://doi.org/10.1016/S0038-0717(03)00151-2
Prescott CE, Vesterdal L (2021) Decomposition and transformations along the continuum from litter to soil organic matter in forest soils. For Ecol Manag 498:119522. https://doi.org/10.1016/j.foreco.2021.119522
Rao Z, Zhu Z, Jia G, Chen F, Barton L, Zhang J, Qiang M (2010) Relationship between climatic conditions and the relative abundance of modern C3 and C4 plants in three regions around the North Pacific. Chin Sci Bull 55:1931–1936. https://doi.org/10.1007/s11434-010-3101-z
Rao Z, Chen F, Zhang X, Xu Y, Xue Q, Zhang P (2012) Spatial and temporal variations of C3/C4 relative abundance in global terrestrial ecosystem since the last glacial and its possible driving mechanisms. Chin Sci Bull 57:4024–4035. https://doi.org/10.1007/s11434-012-5233-9
Rask HM, Schoenau JJ (1993) 13 C natural abundance variations in carbonates and organic carbon from boreal forest wetlands. Biogeochemistry 22:23–35. https://doi.org/10.1007/BF00002755
Reisser M, Purves RS, Schmidt MWI, Abiven S (2016) Pyrogenic Carbon in Soils: a literature-based inventory and a global estimation of its content in Soil Organic Carbon and stocks. Front Earth Sci 4. https://doi.org/10.3389/feart.2016.00080
Roscoe R, Buurman P, Velthorst E, Vasconcellos C (2001) Soil organic matter dynamics in density and particle size fractions as revealed by the 13 C/12 C isotopic ratio in a Cerrado’s oxisol. Geoderma 104:185–202. https://doi.org/10.1016/S0016-7061(01)00080-5
Rubino M, Etheridge D, Thornton D, Allison C, Francey R, Langenfelds R, Steele P, Trudinger C, Spencer D, Curran M, van Ommen T, Smith A (2019) Law Dome Ice Core 2000-Year CO2, CH4, N2O and d13C-CO2. CSIRO
Ruiz-Dueñas FJ, Martínez AT (2009) Microbial degradation of lignin: how a bulky recalcitrant polymer is efficiently recycled in nature and how we can take advantage of this. Microb Biotechnol 2:164–177. https://doi.org/10.1111/j.1751-7915.2008.00078.x
Šantrůčková H, Bird MI, Lloyd J (2000) Microbial processes and carbon-isotope fractionation in tropical and temperate grassland soils. Funct Ecol 14:108–114. https://doi.org/10.1046/j.1365-2435.2000.00402.x
Šantrůčková H, Kotas P, Bárta J, Urich T, Čapek P, Palmtag J, Eloy Alves RJ, Biasi C, Diáková K, Gentsch N, Gittel A, Guggenberger G, Hugelius G, Lashchinsky N, Martikainen PJ, Mikutta R, Schleper C, Schnecker J, Schwab C, Shibistova O, Wild B, Richter A (2018) Significance of dark CO2 fixation in arctic soils. Soil Biol Biochem 119:11–21. https://doi.org/10.1016/j.soilbio.2017.12.021
Schiedung M, Don A, Beare MH, Abiven S (2023) Soil carbon losses due to priming moderated by adaptation and legacy effects. Nat Geosci. https://doi.org/10.1038/s41561-023-01275-3
Schmidt MWI, Torn MS, Abiven S, Dittmar T, Guggenberger G, Janssens IA, Kleber M, Kögel-Knabner I, Lehmann J, Manning DAC, Nannipieri P, Rasse DP, Weiner S, Trumbore SE (2011) Persistence of soil organic matter as an ecosystem property. Nature 478:49–56. https://doi.org/10.1038/nature10386
Schmidt O, Dyckmans J, Schrader S (2016) Photoautotrophic microorganisms as a carbon source for temperate soil invertebrates. Biol Lett 12:20150646. https://doi.org/10.1098/rsbl.2015.0646
Schneckenberger K, Kuzyakov Y (2007) Carbon sequestration under Miscanthus in sandy and loamy soils estimated by natural 13 C abundance. J Plant Nutr Soil Sci 170:538–542. https://doi.org/10.1002/jpln.200625111
Schneider F, Amelung W, Don A (2021) Origin of carbon in agricultural soil profiles deduced from depth gradients of C:N ratios, carbon fractions, δ13C and δ15N values. Plant Soil 460:123–148. https://doi.org/10.1007/s11104-020-04769-w
Schubert BA, Jahren AH (2012) The effect of atmospheric CO2 concentration on carbon isotope fractionation in C3 land plants. Geochim Cosmochim Acta 96:29–43. https://doi.org/10.1016/j.gca.2012.08.003
Schubert BA, Jahren AH (2015) Global increase in plant carbon isotope fractionation following the last glacial maximum caused by increase in atmospheric pCO2. Geology 43:435–438. https://doi.org/10.1130/G36467.1
Schweizer M, Fear J, Cadisch G (1999) Isotopic (13 C) fractionation during plant residue decomposition and its implications for soil organic matter studies. Rapid Commun Mass Spectrom 13:1284–1290. https://doi.org/10.1002/(SICI)1097-0231(19990715)13:13<1284::AID-RCM578>3.0.CO;2-0
Shi Z, Allison SD, He Y, Levine PA, Hoyt AM, Beem-Miller J, Zhu Q, Wieder WR, Trumbore S, Randerson JT (2020) The age distribution of global soil carbon inferred from radiocarbon measurements. Nat Geosci 13:555–559. https://doi.org/10.1038/s41561-020-0596-z
Smith P, Chapman SJ, Scott WA, Black HIJ, Wattenbach M, Milne R, Campbell CD, Lilly A, Ostle N, Levy PE, Lumsdon DG, Millard P, Towers W, Zaehle S, Smith J (2007) Climate change cannot be entirely responsible for soil carbon loss observed in England and Wales, 1978–2003. Glob Change Biol 13:2605–2609. https://doi.org/10.1111/j.1365-2486.2007.01458.x
Spohn M, Müller K, Höschen C, Mueller CW, Marhan S (2020) Dark microbial CO2 fixation in temperate forest soils increases with CO2 concentration. Glob Change Biol 26:1926–1935. https://doi.org/10.1111/gcb.14937
Staddon PL (2004) Carbon isotopes in functional soil ecology. Trends Ecol Evol 19:148–154. https://doi.org/10.1016/j.tree.2003.12.003
Stevenson BA, Kelly EF, McDonald EV, Busacca AJ (2005) The stable carbon isotope composition of soil organic carbon and pedogenic carbonates along a bioclimatic gradient in the palouse region. Wash State USA Geoderma 124:37–47. https://doi.org/10.1016/j.geoderma.2004.03.006
Swap RJ, Aranibar JN, Dowty PR, Gilhooly WP, Macko SA (2004) Natural abundance of 13 C and 15 N in C3 and C4 vegetation of southern Africa: patterns and implications. Glob Change Biol 10:350–358. https://doi.org/10.1111/j.1365-2486.2003.00702.x
Torn MS, Lapenis AG, Timofeev A, Fischer ML, Babikov BV, Harden JW (2002) Organic carbon and carbon isotopes in modern and 100-year-old-soil archives of the russian steppe. Glob Change Biol 8:941–953. https://doi.org/10.1046/j.1365-2486.2002.00477.x
Torn MS, Swanston CW, Castanha C, Trumbore SE (2009) Storage and turnover of organic matter in soil. In: Huang P, Senesi N (eds) Biophysico-chemical processes involving natural nonliving organic matter in environmental systems. John Wiley & Sons Inc, pp 219–272
Trouve C, Mariotti A, Schwartz D, Guillet B (1994) Soil organic carbon dynamics under Eucalyptus and Pinus planted on savannas in the Congo. Soil Biol Biochem 26:287–295. https://doi.org/10.1016/0038-0717(94)90169-4
Trumbore SE, Schiff SL, Aravena R, Elgood R (1992) Sources and Transformation of dissolved Organic Carbon in the Harp Lake Forested Catchment: the role of soils. Radiocarbon 34:626–635. https://doi.org/10.1017/S0033822200063918
Uzaki M, Mizutani H, Wada E (1991) Carbon isotope composition of CH4 from rice paddies in Japan. Biogeochemistry 13. https://doi.org/10.1007/BF00002775
Valentine DL, Chidthaisong A, Rice A, Reeburgh WS, Tyler SC (2004) Carbon and hydrogen isotope fractionation by moderately thermophilic methanogens. Geochim Cosmochim Acta 68:1571–1590. https://doi.org/10.1016/j.gca.2003.10.012
Walder F, Niemann H, Natarajan M, Lehmann MF, Boller T, Wiemken A (2012) Mycorrhizal networks: common goods of plants shared under unequal terms of trade. Plant Physiol 159:789–797. https://doi.org/10.1104/pp.112.195727
Wang W, Liu X, Shao X, Leavitt S, Xu G, An W, Qin D (2011) A 200 year temperature record from tree ring δ13 C at the Qaidam Basin of the Tibetan Plateau after identifying the optimum method to correct for changing atmospheric CO2 and δ13C. J Phys Res 116. https://doi.org/10.1029/2011JG001665
Wang B, An S, Liang C, Liu Y, Kuzyakov Y (2021) Microbial necromass as the source of soil organic carbon in global ecosystems. Soil Biol Biochem 162:108422. https://doi.org/10.1016/j.soilbio.2021.108422
Werth M, Kuzyakov Y (2010) 13 C fractionation at the root–microorganisms–soil interface: a review and outlook for partitioning studies. Soil Biol Biochem 42:1372–1384. https://doi.org/10.1016/j.soilbio.2010.04.009
Wynn JG, Bird MI, Wong VN (2005) Rayleigh distillation and the depth profile of 13 C/12 C ratios of soil organic carbon from soils of disparate texture in Iron Range National Park, Far North Queensland, Australia. Geochim Cosmochim Acta 69:1961–1973. https://doi.org/10.1016/j.gca.2004.09.003
Xia Z, Zheng Y, Stelling JM, Loisel J, Huang Y, Yu Z (2020) Environmental controls on the carbon and water (H and O) isotopes in peatland Sphagnum mosses. Geochim Cosmochim Acta 277:265–284. https://doi.org/10.1016/j.gca.2020.03.034
Zhang J, Quay PD, Wilbur DO (1995) Carbon isotope fractionation during gas-water exchange and dissolution of CO2. Geochim Cosmochim Acta 59:107–114. https://doi.org/10.1016/0016-7037(95)91550-D
Zhao F-J, Spiro B, McGrath SP (2001) Trends in 13 C/ 12 C ratios and C isotope discrimination of wheat since 1845. Oecologia 128:336–342. https://doi.org/10.1007/s004420100663
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Krüger, N., Finn, D.R. & Don, A. Soil depth gradients of organic carbon-13 – A review on drivers and processes. Plant Soil 495, 113–136 (2024). https://doi.org/10.1007/s11104-023-06328-5
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DOI: https://doi.org/10.1007/s11104-023-06328-5