Abstract
Background and aim
Phosphorus (P) addition is a common practice to alleviate P limitation in agricultural ecosystems. However, information regarding microbial communities’ response to P addition in grasslands remains limited. The present study aimed to investigate the response of bacterial communities from rhizosphere and root endosphere of Achnatherum inebrians to P addition, and assess the potential roles played by mutualistic endophyte Epichloë gansuensis in these processes.
Methods
The response of bacterial communities to P addition was investigated based on 16S rRNA sequencing. Soil properties were determined and Mantel test was employed to evaluate the main factors contributing to bacterial community alteration. Additionally, the root exudates were assessed by GC-MS.
Results
P addition influenced the bacterial community composition in both the rhizosphere and root endosphere of A. inebrians with (E+) or without (E−) E. gansuensis, while not affecting community diversity. Moreover, P addition increased the soil available P, total P, and pH levels, which exhibited significant correlation with the bacterial communities in both rhizosphere and root endosphere of A. inebrians. Additionally, P addition increased the exudation of xylose, glycine, alanine, mandelic acid, and lactic acid from E+ plant roots.
Conclusions
P addition shapes the bacterial communities in rhizosphere and root endosphere of A. inebrians by altering soil total P, available P, pH levels. Meanwhile, P addition modulates the root exudate profiles that mediated by E. gansuensis. This study provides new insights into the response of plant-soil-microbe ecosystem to P addition, which is helpful for the fertilization management during grassland sustainability.
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Funding
This research was supported by the Natural Science Foundation of China (32001399), the Joint Fund of the Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province (Grant No. U1812401), Natural Science Foundation of Gansu Province (22JR5RA451), Changjiang Scholars and innovative Research Team in University (IRT_17R50), the Fundamental Research Funds for the Central Universities (lzujbky-2021-ey01, lzujbky-2021-kb12) in Lanzhou University, the Open Project of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University (2021-KF-02), Technical Service Contract of Microbiology Mechanism in the Process of Themeda japonica Adapting to Different Grades of Rocky Desertification [21(0520)].
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Jianfeng Wang, Chao Wang, Jie Jin conceived the ideas and designed the experiment; Rong Tang, Rong Zheng, Rong Huang collected and analyzed the samples; Jianfeng Wang, Xueli Niu, Yang Yang, Chengzhou Zhao analyzed the data; Jianfeng Wang, Chao Wang prepared the Figures and wrote the manuscript; Yang Yang, Kamran Malik revised and edited the draft.
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Wang, C., Wang, J., Niu, X. et al. Phosphorus addition modifies the bacterial community structure in rhizosphere of Achnatherum inebrians by influencing the soil properties and modulates the Epichloë gansuensis-mediated root exudate profiles. Plant Soil 491, 543–560 (2023). https://doi.org/10.1007/s11104-023-06133-0
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DOI: https://doi.org/10.1007/s11104-023-06133-0