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Effect of phosphorus addition on total and alkaline phosphomonoesterase-harboring bacterial populations in ryegrass rhizosphere microsites

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Abstract

Rhizobacterial communities may play a crucial role in phosphorus (P) nutrition of plants. However, our knowledge of how P fertilization modulates rhizobacterial communities in crops and pastures is still poor. Here, we investigated the effect of P addition (phosphate [PHO] and phytate [PHY]) on the composition of total bacterial communities and alkaline phosphomonoesterases (APase)-harboring bacterial populations in the rhizosphere microsites (root tip [RT] and mature zone [MZ]) of L. perenne. Sizes and diversities of bacterial communities were studied by 454-pyrosequencing of 16S rRNA genes, denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR). Our results suggested that phosphorus addition induces significant changes in the rhizobacterial community composition. Despite that pyrosequence analysis showed that members of the Proteobacteria, Actinobacteria, Chloroflexi, and Acidobacteria were the dominant phyla in all sampled rhizosphere microsites, differences in the relative abundances of some bacterial genera were detected (e.g., Arthrobacter and Acidothermus). Greater richness in rhizosphere microsites of plants supplied with PHY compared with PHO were revealed. With respect to APase-harboring bacterial populations, DGGE (phoD gene) showed significant differences between microsites supplied with PHO, PHY and controls. qPCR (16S rRNA genes, phoD and phoX) showed significantly greater abundances of bacteria and APase genes in RT than in MZ microsites. This study contributes to our understanding of the effect P fertilization on rhizobacterial community compositions of pastures grown in Chilean Andisols.

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Acknowledgments

The authors wish to thank the Editor and three anonymous referees for helpful and constructive criticism. This study was supported by Fondecyt Projects (no. 1120505 and 1141247), International Cooperation Project Conicyt-USA (code USA2013-0010), and Conicyt-MEC (no. 80140015). J.J. Acuña thanks the Fondecyt Postdoctoral Fellowship no. 3140620 and L. Lagos thanks the Conicyt Ph.D. Scholarships no. 21120698.

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Authors and Affiliations

  1. Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile

    Lorena M. Lagos

  2. Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Ave. Francisco Salazar 01145, Temuco, Chile

    Jacquelinne J. Acuña, María de la Luz Mora & Milko A. Jorquera

  3. Department of Microbiology, Graduate School of Medicine, Kyoto University, Yoshida–Konoe–cho, Sakyo–ku, Kyoto, 606-8501, Japan

    Fumito Maruyama

  4. Soil and Water Science Department, University of Florida, PO Box 110290, 2181 McCarty Hall, Gainesville, Florida, 32611, USA

    Andrew Ogram

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  1. Lorena M. Lagos
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  2. Jacquelinne J. Acuña
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  3. Fumito Maruyama
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Correspondence to Milko A. Jorquera.

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Lagos, L.M., Acuña, J.J., Maruyama, F. et al. Effect of phosphorus addition on total and alkaline phosphomonoesterase-harboring bacterial populations in ryegrass rhizosphere microsites. Biol Fertil Soils 52, 1007–1019 (2016). https://doi.org/10.1007/s00374-016-1137-1

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