Abstract
Aims
The improvement of biological nitrogen fixation (BNF) is beneficial to improving legume yield and soil fertility. Thus, the potential of a white-rot fungus Ceriporia lacerata HG2011 in promoting soybean BNF and yield along with the mechanisms was investigated.
Methods
Fungal metabolites and the effects of C. lacerata HG2011 on rhizobial growth, chemotaxis, and biofilm formation were investigated through pure culture. Soybeans inoculated with this fungus were grown in the climatic cabinet, greenhouse, and field for measuring nodulation parameters, BNF-related enzyme activity, BNF, and yield.
Results
Although C. lacerata HG2011 had no significant influence on the growth of the rhizobium Ensifer fredii S2-1, the rhizobial chemotaxis and biofilm formation were improved by this fungus broth in pure culture. C. lacerata HG2011 dwelled on the root surface, increased root tips and surface area, produced the nodulation-associated metabolites (phytohormones, flavonoids, and low-molecular-weight organic acids) and hydrolases (cellulase, pectinase, protease, and phosphatase), mobilized soil P and Fe, and stimulated the activities of plant BNF-related enzymes (nitrogenase, glutamine synthetase, and dehydrogenase). Compared with fungus-free control plants, C. lacerata HG2011 improved nodulation and increased %Ndfa by 3.61–4.64%, BNF by 29.26–40.68%, nutrients uptake (N, P, and K) by 4.38–19.60%, and mean grain yield by 10.55% in the fields.
Conclusion
C. lacerata HG2011 promoted rhizobial chemotaxis as well as soybean nodulation and BNF via releasing phytohormones and improving mineral nutrition. Thus, the fungal inoculant significantly increased soybean yield in both greenhouse and fields, showing a promising application in legume cultivation.
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Data availability
The data that support the findings of this study are available from the corresponding authors upon request.
Abbreviations
- PGPMs:
-
Plant-growth-promoting microbes
- LMWOAs:
-
Low-molecular-weight organic acids
- LC-MS:
-
Liquid chromatography-mass spectrometry
- PDA:
-
Potato dextrose agar
- PDB:
-
Potato dextrose broth
- YMA:
-
Yeast mannitol agar
- YMB:
-
Yeast mannitol broth
- CLB:
-
Ceriporia lacerata HG2011 fermentation broth
- EFB:
-
Ensifer fredii S2-1 fermentation broth
- %Ndfa:
-
The percentage of N derived from the atmosphere
- BNF:
-
Biological nitrogen fixation
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Acknowledgements
This work was funded by Chongqing Science and Technology Commission, Chongqing, China (Project cstc2018jscx-mszdX0011) and the Scientific Innovation Project for Postgraduates, Chongqing, China (Project CYB20097).
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Zongming Sui: Conceptualization, Methodology, Software, Investigation, Formal Analysis, Writing - Original Draft, Funding Acquisition. Ling Yuan: Conceptualization, Resources, Supervision, Writing - Review & Editing, Funding Acquisition.
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Sui, Z., Yuan, L. White-rot fungus Ceriporia lacerata HG2011 improved the biological nitrogen fixation of soybean and the underlying mechanisms. Plant Soil 486, 425–439 (2023). https://doi.org/10.1007/s11104-023-05883-1
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DOI: https://doi.org/10.1007/s11104-023-05883-1