Background and Aims
Pinus cembra represent a typical and important tree species growing in European subalpine and alpine habitats. The ectomycorrhizal (ECM) fungal communities associated to this tree under natural conditions are largely unknown.
In this study, we investigated the ECM fungal abundance and composition at four high-altitude sites (two northern-exposed and two southern-exposed habitats) in South Tyrol (Italy), and included also two different age classes of P. cembra. The ECM partners were characterized morphologically, and identified by rDNA ITS sequence analysis.
The degree of mycorrhization in adult P. cembra was typically 100% in these natural habitats, with a total species diversity of 20 ECM species. The four high-altitude sites were similar concerning their species richness and mycobiont diversity, but they differed significantly in ECM species composition. Young P. cembra had a mycorrhization degree of 100% and a total of 10 species were observed. All mycorrhizal partners of naturally regenerated young P. cembra were only detected in one specific location, with the exception of Cenococcum sp. and Amphinema sp. which were detected at two sites. Young trees harbour a distinct ectomycorrhizal fungal diversity, which is clearly lower than the diversity detected in adult P. cembra trees. The P. cembra bolete (Suillus plorans) is the most important symbiotic partner of P. cembra at Southern Tyrolean high-altitude sites and is known for its strict, species-specific host association.
The ectomycorrhizal fungal community composition strongly depends on geographic region and on the slope exposure (north or south) of the site.
Pinus cembra L. (Swiss stone pine) is a tree species typical of subalpine / alpine European habitats that is often considered a glacial relict of high conservation value. This five-needle pine is well adapted to high-altitude ecosystems within an elevation range between 1500 and 2500 m above sea level (a.s.l.) (Casalegno et al. 2010) where harsh environmental conditions like low winter temperatures, elevated ozone levels, and radiation persist. Pinus cembra is an important tree species of the Alpine timberline ecotone (Ulber et al. 2004; Apetrei et al. 2011) where growth is clearly limited by temperature. In fact, this tree does not survives in areas with mean daily air temperatures above 6–8 °C during the vegetation period (Oberhuber 2004; Rossi et al. 2007) giving a competitive advantage over Picea abies, which otherwise dominates the subalpine vegetation zone of the Alps and the Prealps. The substratum type is not particularly significant for P. cembra, which grows both in calcareous or siliceous conditions. The bird Nucifraga caryocatates acts as the main dispersal vector of P. cembra seeds (Caudullo and de Rigo 2016), and may play an important role in the upward shift of the tree due to increasing rates of global warming (Holtmeier and Broll 2005).
Pinus cembra trees are usually strongly mycorrhized (Keller 1989; Göbl and Ladurner 2000). The host specificity of fungal symbiotic partners is highly variable: generalists form symbioses with a wide variety of plants, whereas specialists associate with plants of only one genus or species. The advantage of host-specialism lies in the highly efficient nutrient and water transfer between the symbiosis partners, and in the exclusion of mycoheterotrophy (Simard et al. 2012). Therefore, host-specialists are often the dominating ectomycorrhizal (ECM) species in high-altitude areas with extreme conditions (Moser 1958; Heumader 1992). Three basidiomycete species of the genus Suillus form a strictly host-specific symbiosis with P. cembra trees in these regions: S. plorans, S. placidus, and S. sibiricus (Göbl and Ladurner 2000; Bacher et al. 2010; Rainer et al. 2015). They belong to the largest monophyletic group of ECM fungi that occur exclusively in symbiosis with a single plant family (Pinaceae) (Zhang et al. 2022). These three Suillus species occur in the alpine region exclusively with Swiss stone pines and are therefore called "Swiss pine boletes". The Swiss pine boletus (S. plorans (Roll.) Sing.), the ivory boletus (S. placidus (Bon.) Sing.) and the ringed Swiss pine boletus (S. sibiricus (Sing.) Sing.) differ physiologically by different site and nutrient requirements (Keller 1996). Suillus mycorrhizae are characterized by their initially coralloid, then nodule-like form, and extensive mycelial network attached to the mycorrhiza (Göbl and Ladurner 2000; Rainer et al. 2015). With thick strands of hyphae (rhizomorphs), P. cembra boletes traverse the substrate and cover long distances in search of nutrients and water, thus they are considered to be of the "long-distance exploration type" according to Agerer (2001). Due to host specificity, these mycelial networks exclusively connect plants of the same species (Kennedy et al. 2007), potentially allowing nutrient translocations between different tree individuals (e.g., from old to young stone pines). Host specialization prevents material flows to other tree species (interspecific epiparasitism / mycoheterotrophy). Besides Suillus species host-specialist, there are a number of generalist ECM fungi that are associated with P. cembra. For example, Amphinema byssoides, Thelephora terrestris or Cortinarius species, are ECM symbionts whose function ranges from trophic interactions to protective effects (Selosse et al. 2004), but they are usually occurring in low abundance only (Kranabetter 2004; Rainer et al. 2015). The mycobiont species composition can change during the tree development, and seedlings may have a different species composition than adult trees (Visser 1995; Trocha et al. 2006; Bacher et al. 2010).
Up to the present, the knowledge concerning ECM communities of P. cembra is either based on empirical data concerning fruiting body occurrence (Favre 1960; Horak 1963; Keller 1989), mycorrhizal morphotyping (Göbl and Ladurner 2000), or ECM communities from afforestations (Moser 1963; Rainer et al. 2015) and nurseries (Bacher et al. 2010). The ECM fungi associated to P. cembra in an afforestation in North Tyrol (Austria) were long-term monitored, with the aim of confirming the sustainability of ECM inoculation with Swiss pine boletes, which had been carried out in 1960. The results documented only small differences in the species composition between young and adult trees in this afforestation, with the ECM communities strongly resembling the original Suillus species inoculum (Göbl and Ladurner 2000; Rainer et al. 2015). However, the ECM communities of young and adult P. cembra trees in natural forests remain largely unknown. Thus, this study aims to identify the ECM symbiotic partners of P. cembra in different natural Southern Tyrolean forests. Specifically, we wanted to ask the following questions: (i) What is the ECM status of natural Swiss stone pines? (ii) Are there different ECM fungal communities characteristic for the different age-stages? (iii) Are there ECM fungi characteristic for P. cembra habitats in general, or are there fungal indicator species adapted to specific habitat conditions? We hypothesize that some ECM partners are ubiquitous host-specialists to P. cembra, but that other fungal partners are specific to the location, environment and climatic conditions.
Materials and methods
Study site description
Two different regions in South Tyrol (Italy) were chosen to carry out the study on ECM fungal communities in P. cembra root systems, the Pustertal and Vinschgau. These regions have an opposite geographical location in South Tyrol with overall different climate due to wind exposure, annual precipitations, and historical development. For each valley, two different high-altitude P. cembra forest sites were selected (Fig. 1a, Table 1): one on a southern-exposed slope, and one on a northern-exposed slope. Because of the sun direction, slope exposure is an important variable when considering the local climate of the sites: southern slopes are usually drier and warmer, while northern slopes are cooler and moister.
In Pustertal, the area in Ahornach represents the southern-exposed site whereas the area in Weißenbach the northern-exposed one. In the former, the average age of P. cembra was about 80 years, with individual spruces interspersed in the stand. In the latter, the average tree age was about 50–80 years, with single spruces intermixed. In the region of Vinschgau, Kortsch near Schlanders represents the southern-exposed site. Here, the average age of the P. cembra was about 80 years, with interspersed spruces. The northern-exposed forest is located in Latzaun near Kurzras. The average age of this pure P. cembra stand was about 50 years. In all locations, sampling was carried out at the same altitude, at roughly 2000–2100 m above sea level.
The sampling area is typically free of snow between April or May and October or November. Detailed information on the soil properties (https://tirolatlas.uibk.ac.at/maps/interface/topo.py/index?image=c7_boden), mean year temperature, and total year precipitation (https://meteo.provincia.bz.it/default.asp) of the sites are reported in Table 1. Soil samples were taken at each site for pH measurements in 0.01 M CaCl2 as described in Thomas (1996) (Table 1).
Sample collection and mycorrhizal morphotyping
Within each site, two different age stages of P. cembra were identified, namely adults spruce (Adult) and young spruce (Young) based on their height and stem diameter (Fig. 1b).
To sample the ECM fungal community on the root system of adult pines (between ten and fifteen m high) in each of the four sites, 5 soil blocks were randomly taken with a spade in the P. cembra area. The soil blocks had an area of 20 × 20 cm and depth varying between 10 and 20 cm, depending on the subsoil conditions. Care was taken to ensure that the distance to interspersed spruce was as great as possible. Sampling of all sites took place during the last week of September and the first week of October 2008. In order to determine the autochthonous ECM fungal community in young pines, 10 whole individuals were retrieved from each of the four sites in mid-June 2009. The plants were between five and fifteen cm high, and had a maximum stem diameter of 6 cm.
The root systems retrieved from the young stone pines and from the soil blocks were gently washed in tap water over a 2 mm sieve to remove most of the soil and organic debris by hand, minimizing damage to the ectomycorrhizas. Material, which was adhering tightly to the root system, was removed with forceps. All root systems from each stone pine and soil block were distributed in Petri dishes and then a specific number of mycorrhized fine roots were randomly taken and counted. A total of 600 root tips were analysed for each age class and site. The number of analysed root tips associated with young plant varied between 30 and 300.
All observed root tips were distinguished based on morphological criteria following Agerer (2001) and classified into different morphotypes (MT) using a Nikon SMZ800 stereo-microscope at 10—to 100—fold magnification. The following parameters were decisive: colour of the root tip, surface texture (e.g., smooth, granular, tomentose, hairy), presence of detached hyphae or a hyphal web, structure and length of the mycorrhizal axis. For each MT, at least 4 root tips were selected and stored at -20 °C in Eppendorf-tubes containing 50 μl cetyltrimethyl ammonium bromide (CTAB) buffer until DNA extraction. The remaining root tips were placed in 200 μl of 70% ethanol and stored at 4 °C until further processing. Genomic DNA from MT which did not have at least 4 root tips was not extracted, thus not sequenced, and were categorized as “Rare Mycorrhizal tips”.
DNA extraction, amplification and sequencing
Genomic DNA was extracted from individual root tips following Southworth (2000; http://www.sou.edu/ BIOLOGY/Faculty/Southworth/CTAB.htm). For DNA amplification, the following primer combinations were used: ITS1F × LR15, ITS1F × LR21, ITS1F × NL4 and ITS1F × ITS4. Purified PCR products (ExoSAP-IT PCR Clean-up Kit, GE-Healtcare Europe GmbH, Austria) were sent to Genoscreen (Lille, France) for sequence analyses for the ITS1 region. Details for DNA extraction and molecular amplification can be found in Bacher et al. (2010).
Data analysis and statistics
Resulting rDNA ITS sequences were edited and checked using Sequencher (version 4.6; Gene codes Inc. Ann Arbor, MI). Sequences were merged and clustered into operational taxonomical units (OTUs) with a 97% score similarity based on greedy clustering algorithm (agc) using MOTHUR v1.44 (Schloss et al. 2009). Afterwards, the taxonomic affiliation of each unique sequence was obtained using UNITE Fungal ITS train set 10–05-2021 (Abarenkov et al. 2021) with kmers analysis (1000 interactions) on a confidence bootstrap threshold of 80% (classify.seqs). Then, UNITE and GenBank databases (Clark et al. 2016) were used to determine the best match sequence based on the taxonomic affiliation and bootstrap values (Kõljalg et al. 2005) (Supplementary Table S1). Species belonging to the Suillus genus frequently present higher variability in the ITS regions, while still belonging to the same morphological concept species based on the fruiting body material (ID: IB20050424) (Zhang et al. 2022). Thus, when distinct OTUs matched to the same best match sequence in the database, they were annotated according to the best match sequence classification, and distinguished using numbers (e.g., Suillus plorans_1).
Relative occurrence frequencies were calculated by counting how many times a taxon was found across individuals of the same sample (5 individuals for adults, 10 individuals for young). Frequencies are reported in percentage. In the heatmap, clustering analysis for the categorical factors are based on Euclidean distances.
Abundances of morphotypes were converted to abundances of OTUs based on molecular identification. The structure of the ECM fungal community based on counts of each OTU from each individual (biological replicates) across all samples was analyzed by non-metric multidimensional scaling (NMDS), using the Bray–Curtis distance measure to generate the dissimilarity matrix with the R package “vegan” (Oksanen 2015). The amount of variance explainable by the experimental factors (Location and Age) on the ECM fungal community was calculated using permutational multivariate analysis of variance (adonis) function (999 permutations) on Bray–Curtis distance matrix (Oksanen 2015).
For each age stage within each site, proportionate abundance matrices were generated by averaging the number of MT of each OTU across tree replicates and diving it by the total number of MT in each sample. This resulted in an OTU table listing the relative abundance of all the detected OTUs in all of the samples. Alpha diversity indexes and relative abundance graphics were carried out with relative abundance of OTUs. The ECM fungal diversity was expressed using taxa richness, evenness and Shannon’s diversity index (H′) at species level, calculated from the overall relative abundances of taxa for each site and age stage.
All the statistical analyses and graphs (ggplot2) were performed in R (version 4.0.3) (R Team 2013).
ECM fungal diversity increases with tree age and differs among forest locations
Both adult and young trees had a mycorrhization level of 100%. A total of 31 different root morphotypes were differentiated from P. cembra roots of both adult and young trees across all four sample sites. After OTU clustering and taxonomic assignment, a total of 25 unique ECM fungal taxa were identified (Fig. 3). We found no significant changes in total fungal species richness between Pustertal and Vinschgau regions, while there was a strong difference in richness between adult and young trees (F(2,1) = 32, P = 3e-04, Table 2). Moreover, there was a difference only within young trees, between northern and southern slopes (F(2,1) = 4.5, P = 0.01, Table 2). On average, ECM species richness in young trees was almost half-fold lower than in the adult trees (p = 0.001). Twenty ECM taxa were found belonging to adult trees across all sites, while 10 taxa in the young trees. A total of 15 species were unique to the adult and a total of 5 to the young trees (Fig. 4). In addition, beta diversity analyses showed that the species composition of each high-altitude site was relatively homogeneous within tree age, but sites differed greatly from one another (Fig. 2).
ECM fungal composition differed significantly between regions, slope exposure, and age of the trees (Fig. 2). Bray–Curtis distance analyses indicated that regions (Pustertal vs. Vinschgau) imposed the largest effect on community composition, accounting for 17% of the variation in the ECM fungal community composition (R2 = 0.17, p = 0.001). Additionally, sites, the combinatory factor of regions and slope exposure (north vs south), accounted for 15% of the total community composition (R2 = 0.15, p = 0.001). The tree age (adult vs. young) was also a strong explanatory factor, explaining 12% of the variability (R2 = 0.12, p = 0.001), followed by slope exposure with 10% (R2 = 0.102, p = 0.001). The interaction between regions and age, as well as the interaction between age and exposure, accounted for 8% (R2 = 0.0802, p = 0.001) and 5% (R2 = 0.049, p = 0.001) of the variability, respectively. The ECM communities associated with young P. cembra trees tended to cluster together and are significantly different from all other ECM communities of adult trees. Interestingly, the ECM community in young and adult trees of the northern site of Pustertal clustered closer to each other than to the ECM assemblages of other sites.
A few, different taxa dominate the ECM fungal community in each forest site
The most abundant species in adult P. cembra trees in the Pustertal valley were Russula decolorans (44%), Meliniomyces variabilis (21%), Russula paludosa (18%), and Suillus plorans_3 (16%). Sampling at the southern-exposed site Ahornach revealed a mycorrhizal degree of 100%, with a total of 13 morphotypes distinguished and 7 fungal partners identified (Fig. 3). The average number of morphotypes found in a soil block of was 6.40 ± 1.15 per L soil. The most frequent mycorrhizal partners in this site were Russula decolorans (44%), Suillus plorans_1 (9%), Amphinema byssoides (8%), Lactarius necator (8%), and Tylospora asterophora (8%). Sampling at the northern site-exposed Weißenbach revealed a mycorrhizal degree of 100%, with a total of 14 morphotypes distinguished and 6 fungal partners identified (Fig. 3). The average number of morphotypes found was 4.60 ± 0.55 per L soil. The most frequent mycorrhizal partner was Melinomyces variabilis (21%), followed by Russula paludosa (18%), Suillus plorans_3 (16%), Wilcoxina sp. (11%), and Suillus plorans_2 (8%).
The most abundant species in adult trees in Vinschgau valley were Rhizopogon roseolus (37%), L. porninsis (35%), and R. adusta (31%). Sampling at the southern-exposed site Kortsch revealed a mycorrhization level of 100%, with a total of 13 morphotypes and 7 fungal partners identified (Fig. 3). The average number of morphotypes detected in a soil block was 4.20 ± 0.45 per L soil. The most frequently detected taxon was L. necator (22%). Other important mycobionts were Amphinema sp. (19%), L. porninsis (18%), and R. roseolus (15%). Sampling at the northern-exposed site Latzaun revealed a mycorrhization level of 100%, with a total of 11 morphotypes distinguished and 7 fungal partners identified (Fig. 3). The average number of morphotypes found was 5.00 ± 0.71 per L soil. The most frequent mycorrhizal partners were R. adusta (31%), R. roseolus (22%), L. porninsis (17%), and A. muscaria (16%).
In contrast to the ECM fungal community of adult trees, the mycobionts composition on young trees was much simpler. In Pustertal valley, Cenococcum sp. (50%), S. plorans_2 (42%), R. decolorans (29%), and Cortinarius aurantiobasis (21%) were the most abundant mycorrhizal partners of the trees. In the southern-exposed site Ahornach, three species could be distinguished: Cenococcum sp. (50%), R. decolorans (29%), and C. aurantiobasis (21%). On average, 2.20 ± 0.42 of morphotypes per plant were detected. In the northern site Weißenbach, 4 species could be distinguished, with on average 3.60 ± 0.52 morphotypes per plant (Fig. 3). The most frequently detected mycorrhizal partner was S. plorans_2 (42%). Suillus plorans_1 (17%), Melinomyces variabilis (15%), and Helotiales sp. (11%) were also very abundant.
In the young trees of Vinschgau valley, Amphinema sp. (82%) dominated the stone pine roots in both the northern and southern slopes (37% and 45%, respectively). In the southern-exposed site Kortsch, 6 morphotypes and only 1 species could be distinguished, with an average of 2.80 ± 0.92 morphotypes per plant detected, while in the northern-exposed site Latzaun 6 morphotypes and 4 species could be distinguished, with an average of 1.40 ± 0.51 morphotypes per plant (Fig. 3). In this site, also Cenococcum sp. (33%), Cortinarius illuminus (17%), and Molisia sp. (8%) were also identified.
Little resemblance was found across sites when considering the species compositions of the trees (Fig. 4). The few exceptions regarding the northern-exposed site of Pustertal, where M. variabilis and Suillus plorans_2 were found both in the adult and in the young trees in high abundances. In the southern-exposed site, only Russula decolorans was shared between different tree ages. No taxa were commonly found both in the northern-exposed and in the southern-exposed slopes, at any age stages. ECM communities in Vinschgau were the most distinct. In fact, with the exception of Amphinema sp., no taxa were found both in the adult and in the young trees, at any slope exposure. Nevertheless, there was a stronger similarity in species composition between the northern and southern sites in Vinschgau of adult trees compared to Pustertal, with at least three species found on both slope types: Rhizopogon roseolus, L. porninsis, and S. plorans_1. Across regions, very few ECM species were detected both in Vinschgau and Pustertal (Fig. 4). In the adult trees, Russula adusta, and S. plorans_1, while only Cenococcum sp. was found in both regions in the younger trees. S. plorans_1 was the most frequently and abundantly occurring mycobiont out of 25 fungal species associated to P. cembra at both regions.
Local distribution of ECM partners depends on the forest location site
Cluster analysis showed that the ECM fungal communities converged into three main clusters. Sites in Vinschgau and the southern-exposed site of Pustertal clustered together in two separated groups based on the age of the tree, while the ECM communities of Pustertal northern-exposed site grouped together for both adult and young tree communities, thus showing a unique and independent community structure (Fig. 5). In fact, beside S. plorans_1, the mycobiont assemblage of the tees was distinct for each location.
The spatial distribution of most ECM partners across P. cembra individuals within a single location was highly wide-spread (Fig. 5). In fact, the majority of taxa (e.g., Suillus spp., Lactarius spp., Rhizopogon sp.) were found on more than 60% of the adult individual trees. Cortinarius spp., Hydnotrya cerebriformis, Inocybe whitei, Russula adusta, Molisia sp., and S. sibiricus were found in less than 50% of individuals, thus showing a very localized distribution across the forest ground.
Mycorrhizal status of adult Swiss stone pine at natural high altitudinal sites
During the present study, a total of 20 fungal species were identified as fungal partners of adult P. cembra at high-altitude natural forests in South Tyrol. Nine fungal species were already reported as ECM partners from a pilot study on ECM fungi occurring on 20–35 years old P. cembra afforestation in Haggen (Schmid et al. 2006; Rainer et al. 2015). Thus, a total of 30 mycorrhizal partners of P. cembra at such sites are now known.
The ECM symbiosis appears to be important for trees at high-altitude sites. In fact, the P. cembra roots investigated show a very high mycorrhization degree, coherent with the afforestation site of Haggen (Rainer et al. 2015). Although species richness, species distribution (evenness), and diversity were very similar among sites (5–9 fungal partners), adult P. cembra had unique ECM communities within each site. The P. cembra bolete (Suillus plorans) was the only fungal partner present in all locations investigated, in the afforestation in Haggen (Rainer et al. 2015), and in the afforestation in Venet near Zams (Moser 1963; Schmid et al. 2006), and thus can be considered the most important and widespread symbiotic partner of P. cembra in this area. Several species of the genera Lactarius, Russula, and of Rhizopogon were also frequently found, but usually had a more site-specific distribution.
A comparison with the mycorrhizal partners of a 20–35 years old P. cembra forest in the afforestation site in Venet near Zams (North Tyrol, Austria) and in Haggen (North Tyrol, Austria) provides information on the sustainability and suitability of mycorrhizal inoculations. The inoculation of the P. cembra in both locations was done with a mixture of the three P. cembra bolete species (S. placidus, S. plorans, S. sibiricus) (Moser 1958; Göbl 1963). All three species of Suillus have persisted in the habitats as P. cembra mycorrhizal partners, with S. plorans predominating (Schmid et al. 2006; Rainer et al. 2015). The uneven distribution of the different species of Suillus sp. is based on their different substrate and nutrient requirements (Keller 1996). Thus, for a successful mycorrhizal inoculation, the correct selection of the inoculated material is crucial. Both the fungal species and the inoculum provenances were carefully and correctly selected for the afforestation in Venet and in Haggen. In fact, they corresponded to the natural mycorrhizal distributions of optimally growing P. cembra at high-altitude sites where S. plorans and S. sibiricus also occurred, with S. plorans clearly dominant. S. placidus was never detected in our investigation, but its favourable role for the establishment of the tree in these environments cannot be excluded.
The fungal diversity of species detected as ectomycorrhizae on P. cembra root tips is within the range of the mycobiont richness as reported for other ectotrophic plants of the same altitudinal zone in the Alps with subalpine /alpine vegetation: for example, 22 species were reported for Larix decidua (Leski et al. 2008), 28 for Picea abies in Swiss forests (Peter et al. 2001), 39 for Arctostaphlylos uva-ursi (Krpata et al. 2007), and 19 for Salix herbacea growing in the alpine zone (Mühlmann and Peintner 2008). This diversity of fungi associated to P. cembra includes three host specialists (Suillus spp.). All other mycorrhizal fungi are either generalists or have a wider host range. Interestingly, our data also indicate that host shifts are comparatively frequent in these subalpine habitats. We detected several fungal species known as host specialists to other plant partners: Lactarius porninsis (Larix decidua) (Nuytinck and Verbeken 2007), and Rhizopogon roseolus (Pinus mugo, P. sylvestris) (Kipfer et al. 2012). Host shifts are likely to occur at the distributional margin of one partner. In this case, L. decidua, P. mugo, and P. sylvestris habitats are partly overlapping, and the switching to P. cembra hosts enables the fungal partner to escape from habitat restriction into a new and larger habitat.
Suillus plorans as a "multi-stage" mycorrhizal partner and important host specialist
The fungi identified as symbionts of P. cembra can be divided into three different groups: "early-stage” fungi, "multi-stage” fungi, and "late-stage” fungi (Visser 1995). "Early-stage” fungi form symbioses mainly with seedlings and young plants, while “multi-stage” fungi accompany the host plant from the young stage to old age. In contrast, “late-stage” fungi preferentially occur in the adult stage of the plant (Jackson and Mason 1984). In recent studies, S. plorans as well as S. sibiricus occurred on P. cembra trees of all age classes and can therefore be described as typical "multi-stage” fungi (Bacher et al. 2010; Rainer et al. 2015). However, only S. plorans dominated in the Southern Tyrolean P. cembra forests investigated.
Cenococcum sp., Cortinarius sp., Amphinema sp., and Helotiales sp. were unique to the mycorrhizal assemblage of the young P. cembra investigated, and strongly resembled the ECM communities found on the seedlings of L. decidua, P. abies and P. cembra in a previous study carried out in nursery plants (Bacher et al. 2010). Their presence in middle-aged P. cembra afforestation (Schmid et al. 2006; Rainer et al. 2015), makes it difficult to categorize them as “early-stage” fungi. However, they are host generalists and where other trees already exist, their established mycelial network can interact and form symbiosis with the new seedlings in an afforestation. This means that ECM colonization of new seedling roots can occur if other subalpine trees exist on enough in the site.
Furthermore, ECM fungal diversity of P. cembra increased with host development, due to the addition of species-rich fungal lineages such as Lactarius sp. and Russula sp., among others. This is in accordance with the successional pattern of ECM communities in temperate and alpine pine systems, where these species become the major components of the ECM assemblages. Suillus sp. and Rhizopogon sp. have the predominant role in seedling establishment, while the observed succession of ECM fungi may be driven by the accumulation of organic soil matter as the host develops (Koizumi et al. 2018).
Are there mycorrhizal partners of P. cembra that are characteristic for site conditions?
Soil properties were the major drivers of ECM fungal composition in Pinus pumila alpine forests, with soil pH, electric conductivity, and total soil carbon being the most important factors (Koizumi et al. 2018). Suillus plorans and S. sibiricus are generally optimal partners for naturally-regenerated P. cembra, plants and adult P. cembra, and they were present at all high-altitude sites investigated. These habitats have a siliceous bedrock and are usually characterized by semipodsole soil type with low pH, suggesting a preferential pH range of these species. Rhizopogon spp. and Suillus spp. are responsible for the characteristic ECM morphotypes “Knöllchenmycorrhiza” (Göbl and Ladurner 2000), a typical representative of the long-distance mycorrhizal exploration type (Agerer 2001). These mycobionts, together with other highly abundant species occurring in both, natural forests habitats and afforestations (e.g., Amphinema byssoides), appear to be especially suitable for this special, very stony habitat with high soil heterogeneity.
In their Japanese multi-location study of alpine P. pumila forests, Koizumi and Nara (2020) showed that ECM fungal communities were structured by climate factors (temperature) rather than spatial and soil factors. This is in contrast to other recent studies carried out in the Alps, reporting that soil temperature manipulation in a P. cembra (Rainer et al. 2015) and Picea abies forest (Schindlbacher et al. 2011) did not cause significant changes neither in microbial biomass nor community composition. We speculate that such changes can probably only be detected during long-term studies.
Due to significant differences in the species composition of ECM partners at all high-altitude sites, we speculate that the fungal distribution is highly dependent on the availability of fungal inoculation present at each site. This is in accordance with other studies showing that ECM fungi are found to be patchily distributed at both large (regional) and small (less than 1 m) spatial scales in natural environments (Downie et al. 2021). In fact, no fungal species characteristic of dry or cool sites could be detected. Nevertheless, our results indicate that S. plorans might be negatively affected by drought, or higher mean temperatures, as the occurrence of this important mycobiont is clearly reduced in Vinschgau. In fact, this area is characterized by lower annual precipitations and, together with the southern-exposed sites of Pustertal, may result in generally less humid conditions (lower water availability). Furthermore, an in-vitro study on the growth response of drought-stressed P. sylvestris seedlings, inoculated with the associated host specialists S. granulatus and Rhizopogon roseolus, indicated a positive effect of S. granulatus on shoot growth that was more pronounced under moist than under dry conditions (Kipfer et al. 2012). Environmental factors are also known to limit the distribution of P. cembra, which is then replaced by other tree species. Pinus cembra is sensitive to drought stress mainly in lower altitudes. It grows better in cool/humid, deep and well-drained soils (Caudullo and de Rigo 2016). We speculate that shifts in ECM communities might be indirectly speeding up replacement of P. cembra specialists in these high-altitude habitats: With warmer and drier climate, the highly efficient P. cembra host specialist are replaced by ECM host generalist (e.g., Cenoccoccum, Wilcoxina), which facilitate introgression of other plant hosts in the habitat. Furthermore, the increased occurrence of hypogeous Rhizopogon species at the costs of epigeous S. plorans indicates an adaptation to drier environmental conditions. In fact, the Rhizopogon genus is a sister group to the genus Suillus, and both are known to be host specific to Pinaceae (Grubisha et al. 2002). However, Rhizopogon was shown to predominate over Suillus sp. in dry coniferous forest habitat types dominated by P. sylvestris (Hilszczańska et al. 2019). Finally, our data indicate that Russula paludosa might be considered as an indicator species typical for cool sites. In Austria, this species is restricted to sites with an annual mean temperature below 8–9 °C and Podsol or Braunerde soil type (http://austria.mykodata.net/Taxa_map.aspx?qvtaxIdTaxon=317058&). Along with other species belonging to this genus, it is usually distributed in hygrophilous conifer forests (Sarnari 2005).
Pinus cembra trees have a rich and diverse partnership with ECM fungi. This partnership is characterized by the occurrence of typical host specialists like Suillus placidus and S. sibiricus with species-specific adaptation to P. cembra, and Rhizopogon roseolus, which is known to be host-specific to several Pinus spp. The most distinct ECM fungal composition resulted between young and adult trees, with the latter having higher overall richness and diversity than the young trees.
There are clearly distinct ECM communities clustering individually by site, first, and forming sub-clusters by region and slope-exposure (north and south). The age effect is always present regardless of the environment. This means that, especially for adult P. cembra trees, fungal communities are typically similar within the same region, but differ with the slope-exposure. A convergence between dry sites can be indicated by cluster analysis and occurrence of taxa. ECM community establishment probably took decennia in each of these sites (highly heterogenic soil which developed on block terrain). The fungal distribution is also highly depending on the availability of fungal inoculum. The specific ecological roles of the detected fungal taxa remain an open question, which warrants to be addressed by further research on these important but vulnerable high-altitude habitats.
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We thank Dr. Friederike Göbl for advice during planning the project. We are especially grateful to the Amt für Forstverwaltung and all involved forest officials for their precious and active support, to Paul Zipperle for administrative help, and Kurt Stolzlechner for support in the Pustertal. Thanks to Irmgard Oberkofler and Regina Kuhnert for their help in the lab and Maraike Probst for her advice over the statistical analysis.
Open access funding provided by University of Innsbruck and Medical University of Innsbruck. This work was funded by the Autonomous Provinz Bozen (Mycorrhizaprojekt Lärche und Zirbe in Südtirol: P7180-017–013) which provided financial support to MB, and by the FWF (MICINSNOW project: 310380) providing financial support to EM.
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Mandolini, E., Bacher, M. & Peintner, U. Ectomycorrhizal fungal communities of Swiss stone pine (Pinus cembra) depend on climate and tree age in natural forests of the Alps. Plant Soil (2022). https://doi.org/10.1007/s11104-022-05497-z
- Alpine timberline ecotone
- Cool and dry forests
- Slope exposure