Abstract
Aims
In the Swedish sub-Arctic, mountain birch (Betula pubescens ssp. czerepanovii) forests mediate rapid soil C cycling relative to adjacent tundra heaths, but little is known about the role of individual trees within forests. Here we investigate the spatial extent over which trees influence soil processes.
Methods
We measured respiration, soil C stocks, root and mycorrhizal productivity and fungi:bacteria ratios at fine spatial scales along 3 m transects extending radially from mountain birch trees in a sub-Arctic ecotone forest. Root and mycorrhizal productivity was quantified using in-growth techniques and fungi:bacteria ratios were determined by qPCR.
Results
Neither respiration, nor root and mycorrhizal production, varied along transects. Fungi:bacteria ratios, soil organic C stocks and standing litter declined with increasing distance from trees.
Conclusions
As 3 m is half the average size of forest gaps, these findings suggest that forest soil environments are efficiently explored by roots and associated mycorrhizal networks of B. pubescens. Individual trees exert influence substantially away from their base, creating more uniform distributions of root, mycorrhizal and bacterial activity than expected. However, overall rates of soil C accumulation do vary with distance from trees, with potential implications for spatio-temporal soil organic matter dynamics and net ecosystem C sequestration.
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Introduction
Aboveground plant communities and belowground microbial communities, comprised of saprotrophic and mycorrhizal fungi, bacteria and archaea, form intimate and interdependent relationships (Wardle et al. 2004). Ultimately, any changes to aboveground vegetation communities could have cascading belowground effects on microbial communities, carbon (C) sequestration and turnover (Wookey et al. 2009).
The mountain birch (Betula pubescens Ehrh. ssp. czerepanovii (Orlova) Hämet Ahti) forest – tundra treeline ecotone south of Abisko, Sweden, provides a suitable natural system for studying the effect of aboveground vegetation on fundamental soil processes and belowground C cycling. The mosaic of forest and tundra heath across the landscape offers several vegetation transitions, in permafrost-free areas, reducing potential confounding hydrological effects. Previous research at the treeline near Abisko has established that shrub and tree cover have expanded between 1976 and 2009 (Rundqvist et al. 2011), and that there are clear differences in organic C storage between forest and tundra heath soils (Sjögersten and Wookey 2002). Extensive transect work has further shown that the relatively productive mountain birch forest and deciduous shrubs, with higher above-ground C stocks, have significantly less C and nitrogen (N) stored below-ground compared to the relatively less productive adjacent tundra heath (Hartley et al. 2012; Parker et al. 2015). These differences in below-ground C stocks and rates of soil C cycling have been linked to differences in associated belowground mycorrhizal communities between forest and tundra (Parker et al. 2015).
Previous work has focussed on differences in vegetation at the landscape scale, but little is known about the zone of influence of individual trees within treeline forests. Characterising the spatial complexity of soil processes (or lack thereof) in these forests is critical to understanding how soil C cycling may change with potential forest expansion. In a recent study, Parker et al. (2016) observed that respiration was higher at the base of mountain birch trees compared to 150 cm away, indicating ‘hot-spots’ of soil activity close to trees. However, their work lacks further information on possible variation in key soil processes, such as respiration and rhizosphere productivity at finer scales, as well as at greater distances from tree bases. As contrasting vegetation types, and individual birch trees, affect key soil processes (Hartley et al. 2012; Parker et al. 2016), we sought to investigate to what extent individual trees, and their associated root and ectomycorrhizal networks, influence surrounding soil C cycling. We also sought to determine how far these networks extend and, in addition, we deployed indirect techniques to identify any spatial shifts in the balance between autotrophic and heterotrophic soil CO2 efflux (Subke et al. 2006; Subke and Bahn 2010). Quantifying and understanding fine-scale variation in soil processes is particularly relevant in the mesic ecotone mountain birch forests, as many of these are open in structure with large spacing between trees. This sparse density forest is also common for many arctic treelines across North America and Eurasia (Kullman and Öberg 2009; Payette and Lavoie 1994).
Given this ecological context there are two, potentially mutually exclusive, possibilities regarding spatial variation in the mycorrhizosphere within the forest. (i) If the influence of trees is limited in range, then there may be pockets within forests that function more similarly to tundra heath in terms of their soil processes, with lower respiration, high soil C stocks and ericoid fungal dominance (Cornelissen et al. 2001; Read and Perez-Moreno 2003). Alternatively, (ii) if the influence of trees and their associated mycorrhizosphere extends beyond the mean maximum distance between trees, there may be contiguous, or overlapping, networks of ectomycorrhizas exploring and exploiting resources throughout the forest floor. This could indicate the presence of a so called wood-wide-web (Beiler et al. 2010; Simard and Durall 2004; Wiemken and Boller 2002) and the potential for common mycelial networks (CMNs) where mycorrhizas associate with multiple plants, and vice versa (Hazard and Johnson 2018; Simard et al. 2012).
Understanding the coupling between plant and microbial communities in the Arctic, and the consequences for fundamental soil processes, is particularly important due to the large amounts of C stored in high latitude soils (Kuhry et al. 2013; Ping et al. 2008), as well as the substantial allocation of plant biomass below-ground (Iversen et al. 2015). Much of this C is potentially vulnerable to be released to the atmosphere, if temperatures rise, through its metabolism by soil organisms, constituting a potential positive feedback to climate forcing and the acceleration of climate change (Karhu et al. 2014; Luo 2007). The direct interface between plants and soil is the rhizosphere and the maintenance of symbiotic relationships with mycorrhizal fungi which explore the soil for water and nutrients and receive plant photosynthates (Anderson and Cairney 2007; Smith and Read 2008). In the sub-Arctic, the majority of mycorrhizal associations are those of ericaceous sub-shrubs with ericoid mycorrhizas (ERM), and betulaceae (both tree and dwarf birch) with ectomycorrhizas (ECM) (Cornelissen et al. 2001; Read and Perez-Moreno 2003).
The effects of microorganisms and mycorrhizal fungi on ecosystem function and soil C-cycling depend on the growth form and morphotype of individuals in a microbial community (Clemmensen et al. 2015; Cornelissen et al. 2001). These can vary significantly depending on the soil environment and the above-ground plant communities (Grayston and Prescott 2005; Hazard and Johnson 2018). One example is of ericaceous ERM dominated systems capable of significant C sequestration in melanised hyphal biomass vs boreal ECM dominated systems with more rapid biomass turnover and low C sequestration (Clemmensen et al. 2013, 2015). Another example is where soils below ericaceous dwarf shrubs are dominated by fungi, while those below herbaceous vegetation or temperate woodlands are dominated by bacteria (Högberg et al. 2007; Mitchell et al. 2010). This is thought to be related to differences in the quantity and quality of above- and below-ground litter and root exudates, C:N ratios in the soil (Fierer et al. 2009) and soil pH (Mitchell et al. 2010). As fungi have higher C assimilation efficiency (Zhang et al. 2005), and higher recalcitrance of necromass, than bacteria (Six et al. 2006; Strickland and Rousk 2010), fungal dominated soils sequester more C and have lower respiration rates than bacterial dominated soil (Averill and Hawkes 2016; Clemmensen et al. 2013, 2015). Understanding the link between vegetation, soils, and the ecological processes they mediate is key to understanding soil C sequestration and turnover at local and landscape scales.
Given the strong influence of mountain birch trees on soil processes around them, we hypothesise the following:
-
1)
Production of fine roots and ectomycorrhizal hyphae declines significantly at increasing distances from tree bases, reflected in declining contributions of autotrophic inputs towards total respiration.
-
2)
Respiration rates show corresponding patterns of decline with increasing distance from tree bases.
-
3)
The ratio of soil fungi:bacteria increases with distance away from tree bases, creating conditions similar to more open heath.
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4)
The patterns of soil organic carbon stocks will reflect the relative consequences of (a) declining litter deposition, and (b) declining mycorrhizosphere activity, with increasing distances from trees. All other factors remaining equal, the former would tend to contribute to higher C stocks closer to trees (denoted Hypothesis 4a), while the latter would tend to result in slower turnover of soil C, and higher C stocks, further away from trees (Hypothesis 4b).
Materials and methods
Site descriptions
All studied trees were selected within a permafrost-free area (approx. 1 km2) in the sub-Arctic treeline ecotone at Nissunsnuohkki, south of Abisko, Sweden (ca. 68°18′56.2”N 18°49′18.2″E), ~600 m asl. The treeline forest comprises mountain birch (Betula pubescens ssp. czerepanovii) and has an open canopy structure with an ericaceous understorey consisting of Vaccinium vitis-idaea, V. myrtillus and Empetrum nigrum ssp. hermaphroditum. In this area the Betula pubescens above-ground biomass was estimated to be 0.066 ± 0.036 kg m−2 (mean ± SD) and LAI estimated to be 0.17 ± 0.11 m2 m−2 (Dahlberg et al. 2004).
Forest soils are ‘microspodosols’ with a thin O horizon (< 5 cm) underlain by glacial till on a bedrock typically of hard-shale (Sjögersten and Wookey 2002); Soil pH in the organic horizon is 4.3 ± 0.1 (Parker et al. 2015).
Gradients in relation to distance from single birch trees
Tree selection and transect set-up
Three blocks of 3 individual trees (n = 9) were selected for study (Fig. 1). The blocks were within a 500 m radius area and at least 150 m apart. Mountain birch trees of varying size and number of polycormic stems were selected, all located in open forest areas with ≥6 m to any neighbouring trees in the direction of the transect. Care was taken to select various topographical aspects in order to account for variation of influencing factors such as water or snow accumulation. Crown width of all trees was measured in the N-S and E-W orientation and averaged (Supp. Table 2).
Transects were set up in a straight line from the base of each selected tree with measurement locations at 25, 50, 75, 100, 200 and 300 cm from the tree base. Directions of transects were intentionally varied such that each block had transects of differing compass direction to account for shading, prevailing winds or snow drifting, and to allow transects to radiate into open forest gaps.
Digital photographs of each respiration collar were taken from directly above. Image analysis software ImageJ2 (Rueden et al. 2017) was used to determine the percentage vegetation cover of Vaccinium vitis-idaea, Empetrum nigrum and cryptogams in collars.
Soil and understorey C fluxes
At each point along the transects, PVC collars of 15 cm diameter and 6 cm height were secured to the soil surface with the centre of the PVC ring corresponding to a given distance. Non-setting putty (Plumber’s Mait®, Bostik Ltd., Stafford, UK) was used to secure and seal the PVC collars to the soil in order to minimise disturbance of the soil and prevent severing of any roots or fungal hyphae. The forest floor vegetation was parted to allow placement of each collar resulting in vegetation inside and outside the collar (Table 1).
An EGM-4 infrared gas analyser with a darkened CPY-3 chamber (PP Systems International, Amesbury, MA, USA) was used to measure respiration. Respiration in this study is defined as the sum of microbial, root and shoot (including cryptogam) respiration within the chamber, and therefore represents forest soil and understorey respiration. Plants were not initially removed from collars in order to minimise disturbance to the system. Respiration rates were calculated as the slope of a linear function of CO2 concentration increase within the closed system over a period of 96 s. Respiration measurements of all 3 blocks were always completed within 3 h and the order in which the blocks and transects within each block were measured was randomised each time. Measurements were conducted regularly through the full growing season in 2017 from bud swelling (08/06/17) to full leaf-out (23/06/17) and peak growing season (23–26/07/17) through to leaf senescence (22/09/17). A total of 11 repeated measurements were conducted at each collar. Additionally, on June 11th (12:00–16:40 h) CO2 flux measurements were taken with a clear chamber allowing photosynthesis as an estimate of understorey gross primary production (GPP). As the vegetation canopy in the forest is higher than the measurement chamber, respiration measured here is the sum of root, soil heterotroph and limited understorey leaf respiration (65.2 ± 31.3% cover), referred to as respiration. Soil temperature and moisture were measured every hour at 5 cm depth using ONSET (Bourne, MA, USA) 12-Bit Temperature Smart Sensor and EC5 Soil Moisture Smart Sensor, respectively, logged on a HOBO microstation. Temperature response curves were generated for the entire growing season for all plots (seasonal Q10 values); however, data from trees not in full leaf and data from extremely dry days were excluded from the analysis due to the known break-down of the temperature response at the extremes of low moisture (Sjögersten and Wookey 2002). We note that these fluxes incorporate both autotrophic and heterotrophic flux contributions, which vary throughout the growing season. Rather than being inherent temperature sensitivities of soil decomposition, these “virtual” Q10 values (sensu Subke and Bahn 2010), integrate total belowground metabolic activity, with higher values associated with areas of high autotrophic contributions during peak season.
Hyphal in-growth
Hyphal in-growth bags made of 6 × 6 cm 37-μm nylon mesh (allowing hyphal in-growth while excluding roots), and sealed with a heat sealer, were filled with 18 g of sand from the shore of lake Torneträsk (68°21’N,18°49′E) that had been washed, sieved and autoclaved twice before being oven dried. Individual hyphal in-growth bags were deployed on June 12th 2017, 15 cm to the left (when facing the tree) of each PVC collar along each transect, creating a parallel transect. Each hyphal in-growth bag was flattened to ensure an even width of approximately 0.5 cm in each bag. This geometry was chosen to allow hyphae from various mycorrhizal species to grow into and explore through the sand depleted of organic carbon and into the organic carbon rich soil on the other side, thereby avoiding biasing for any particular species according to their characteristic ability to grow in low nutrient environments. Each bag was deployed in the organic soil horizon (just below the litter layer) at a 45° angle from the vertical (both to provide good contact with the soil, but also to avoid any biases associated with lateral or geotropic hyphal growth), thus covering a vertical depth of approximately 4 cm.
All hyphal in-growth bags were harvested on September 17th 2017, resulting in a total of 97 days in the organic soil layer. Bags were transported to the research station within 4 h of retrieval, and all content transferred to sterile plastic bags. Samples were stored at −80 °C for 3 days, then freeze-dried for 72 h in a ModulyoD freeze drier (ThermoFisher Scientific, Waltham, MA, USA). The majority of in-grown hyphae are assumed to originate from ECMs, as found by Wallander et al. (2001).
Hyphae were extracted by suspending 1.5 g of sand in 25 ml deionised water and sonicating the solution for 10 min. The separated hyphae and 10–15 ml water solution were aliquoted into a 15-ml falcon tube to allow mixing and further separation of hyphae from sand. This was then passed through a Büchner funnel with 25-mm glass microfiber filters (Whatman ™). The hyphae-filter matrix was then analysed for carbon content using a FLASH SMART elemental analyser (ThermoFisher Scientific, Waltham, MA, USA) after drying for 72 h at 50 °C. This process was repeated for 8 laboratory blank samples that had not been incubated in the field and the percentage carbon content (0.34 ± 0.05 %C) was subtracted from all samples before further data processing.
Root in-growth
Root in-growth bags of 2 mm plastic mesh were constructed from 8 × 9 cm squares of mesh resulting in a final height of ~5 cm and a cross-sectional area of ~3 cm2 after being filled and sealed using a heat sealer. The bags were loosely filled with organic soil collected from the tundra adjacent to the studied forest plots to mimic the loose structure of the organic soils within the forest. The collected soil was first oven dried at 85 °C for 48 h and sieved to 4 mm with most roots removed, but some small root fragments remaining. The bags were filled with 3.78 ± 0.34 g (mean ± standard deviation) of soil (average amount based on 10 randomly selected filled bags). Once filled, the bags were submerged overnight in deionised water to re-wet the dried soil and minimise loss of soil during transportation and deployment. All root in-growth bags were deployed on June 15th 2017. One root in-growth bag was deployed approximately 15 cm to the right (when facing the tree) of each PVC collar along the transect creating a parallel transect. A 2 cm diameter soil corer was used to extract a soil core to 5 cm depth and the root in-growth bag was inserted into the resulting hole. All root in-growth bags were harvested on September 15th 2017, giving a total of 92 days in the organic layer, incorporating the majority of the root growing season in subalpine birch forests in the Scandinavian subarctic (Blume-Werry et al. 2016). The bags were harvested using surgical grade scalpels to carefully cut any roots growing through the mesh to ensure all new root mass was maintained within the bag. In the lab, any roots protruding out of the mesh were cut off and discarded before the bags were opened; all new roots were collected and washed by finely examining the soil within each bag individually. Roots were not differentiated as originating from birch trees or ericoid shrubs. The new roots were oven dried at 60 °C for 5 days and weighed.
Soil core sampling
Soil cores were taken during root in-growth bag deployment. The full organic horizon depth was measured and the top 5 cm of the organic soil horizon was retained and stored at −18 °C for further analysis. Subsequently, samples were oven dried at 75 °C for 72 h and soil organic matter (SOM) content for each sample determined by loss on ignition in a furnace at 550 °C for 4 h (Ball 1964). Organic soil bulk density for forested areas in the Abisko forests is 0.115 ± 0.043 g cm−3 (mean ± SD, n = 24) and SOM was converted to soil organic carbon (SOC) content using the formula: SOC = SOM*0.5248, parameterized based on extensive data on the relationship between SOM and soil C content around this area (Parker et al. 2015).
Standing litter
Standing litter along transects was collected from a 25 × 25 cm area next to each collar on June 11th 2018. Collected litter was sorted into intact birch leaves and fragmented litter (including some litter from understorey species). The litter was dried at 60 °C for 24 h and weighed.
DNA extraction and qPCR
DNA was extracted from homogenous, representative soil samples using a DNeasy PowerSoil kit (Quiagen, Hilden, Germany) following the manufacturer’s instructions. Extracted DNA was diluted 1:10 for qPCR reactions. Several different sets of primers were tested before the eventual selection of primers below. This selection was based on suitable amplicon length for the standards used. qPCR was run on a Step-One™ RealTime PCR system (Applied Biosystems) using the thermocycle programme described in Fierer and Jackson (2005) for Eub338/Eub518 primers, and May et al. (2001) for NS1/GCfung primers. Gradient PCR was performed on a Veriti 96-Well thermocycler (Applied Biosystems) prior to qPCR to validate the annealing temperatures. Primers used for ‘All fungi’ were NS1 (5′-GTA GTC ATA TGC TTG TCT −3′) (White et al. 1990) and GCFung (with GC clamp removed) (5’-CAT TCC CCG TTA CCC GTT −3′) (May et al. 2001). Primers used for ‘All bacteria’ were Eub338 (5’-ACT CCT ACG GGA GGC AGC AG-3′) and Eub518 (5′-ATT ACC GCG GCT GCT GG-3′) (Fierer and Jackson 2005). Each 20 μl reaction contained 10 μl (2x) PerfecTA SYBR Green Fastmix with ROX (QuantaBio, Maine, USA), 1.25 μl of each forward and reverse primer, 1.25 μl (20 mg ml−1) BSA, 2 μl DNA template and 4.25 μl nuclease free water. Two technical repeats were run per sample. Standards were created using Pseudomonas putida and Saccharomyces cerevisiae DNA with bacterial and fungal primers respectively. The resulting PCR product was cleaned using Wizard® SV Gel and PCR clean-up system (Promega, UK) and quantified by UV spectrophotometer (NanoDrop 2000, Thermo Scientific). Standard DNA was serially diluted by 1:10 and 5 point repeated standard curves were generated for bacteria (109–104, excluding 107) and fungi (107–103). All standard curves had R2 > 0.99. Melt curve analysis was performed for all samples and standards to confirm a single product.
Statistical analysis
Variation in respiration, root production and hyphal production along transects was investigated using a linear mixed effects model (Pinheiro et al. 2017) with block, tree and collar assigned as random effects, accounting for variation between sampling dates. Parametric bootstrapping (×1000) was used to predict 95% confidence intervals of the model. Tree basal area and vegetation cover were removed as covariates from the model as they did not significantly improve the model fit as measured by Akaike Information Criterion (AIC values. SOC content and vegetation cover were analysed using a similar method with block and tree as random effects. Seasonal Q10 values were modelled for each collar individually using the non-linear model fit nls.lm in R package minpack.lm (Elzhov et al. 2016) following the exponential regression: SR = Rate0 Q10(T/10), where SR is fitted soil CO2 efflux, Rate0 is the basic respiration rate at 0 °C (y-axis intercept), Q10 is the fitted temperature sensitivity, and T is soil temperature .
All analyses was carried out using R Version 3.4.0.
Results
Respiration
Respiration showed no significant differences (P = 0.22) between each point measured along transects extending 3 m radially from the base of mountain birch trees. A linear mixed effects model (Fig. 2a) predicts no difference in respiration ≤3 m away from the nearest tree. By contrast, at all points along transects, we found variation by a factor of 2–4 between replicate trees measured.
However, when respiration along transects is presented per unit SOC (Fig. 2b), rather than per unit area, there is a significant (P = 0.0003) increase in respiration per unit SOC with increasing distance from the tree.
The SOC stocks (P = 0.02; Table 1) and total standing litter (P = 0.0009; Supp. Figure 2) are significantly negatively related to distance from the tree. By contrast, understorey gross primary production (GPP), measured on 11 June 2017 as the difference in CO2 production in dark versus full sunlight conditions, did not vary significantly along the 3 m transects (P = 0.94; Table 1).
The composition and total coverage of vegetation within the collars used for respiration measurements along transects did not vary significantly with distance (P = 0.31; Table 1).
Root and hyphal production
There was no significant difference in either total root (P = 0.69) or hyphal (P = 0.14) production along transects over growing season (Fig. 3), with high levels of variation between replicate trees observed in both root and hyphal production. There was no significant difference (P = 0.31) with distance from tree when roots were normalised by % total vegetation cover. Corresponding to patterns observed in root and hyphal production, the seasonal Q10 (Fig. 8), does not vary significantly (P = 0.223) along transects extending from tree bases (Fig. 4).
Soil microbial communities
The quantity of neither total fungi (P = 0.082) nor total bacteria (P = 0.97) varied significantly with distance from trees (Fig. 5a). These data were used to generate a fungi:bacteria ratio along the transect and here, by contrast, there is a significantly decreasing relationship, declining by 0.077 ± 0.035 per m (P = 0.035) with increasing distances from trees (Fig. 5b).
Discussion
Through systematic measurements of key soil processes at varying distances from trees, we found no significant trends in respiration and mycorrhizosphere productivity within mesic mountain birch forests (Hypotheses 1 and 2 are therefore not supported by the data). We did, however, detect a significant increase in the ratio of fungi:bacteria with increasing distance from trees (Fig. 5b), providing support for Hypothesis 3. By contrast, we noted that litter fall and SOC declined significantly with increasing distance from trees (Figs. 2 and 3 and 7, Table 1), supporting Hypothesis 4a, but not 4b. These findings are summarised graphically in Fig. 6.
The lack of significant trends in mycorrhizosphere productivity does not signify homogeneity in these soils, as high levels of variation between studied trees were apparent (Figs. 1 and 2). This lack of a clear spatial trend in mycorrhizosphere productivity, however, is particularly relevant in the context of the open structure of treeline ecotone forests. With 2–7 m between trees (Table 1), a 3 m transect represents the likely maximum distance obtainable from the nearest tree base without being closer to a neighbouring tree. Thus, if root and hyphal production show no significant trends on a scale of up to 3 m with respect to the nearest tree, then it can be hypothesized that this lack of pattern reflects efficient exploitation of the soil resource by root and ECM networks extending throughout these forests (given similar environmental and edaphic contexts). The influence of trees on soil processes such as respiration and mycorrhizosphere productivity up to (and potentially beyond) 3 m away from trees is further supported by similar mean seasonal Q10 values across all measured distances along the transect (Figs. 4 and 8).
The absence of a trend in respiration and mycorrhizosphere productivity with increasing distance from trees within the forest does not support our original Hypotheses 1 and 2. These findings therefore also contrast with those of Parker et al. (2016), where systematic declines in respiration were noted 150 cm away from tree bases compared with 50 cm. It should be noted, however, that Parker et al. (2016) reported fewer fine scale spatial measurements and lower levels of replication than the current work. Our results are also in accordance with 46% of reviewed datasets in a recent meta-analysis across plant communities, which found no significant horizontal spatial variation in ectomycorrhizal communities (Bahram et al. 2015). The lack of spatial variation with respect to respiration and mycorrhizosphere productivity found here may be linked to widespread activity of birch tree roots, ectomycorrhizas and bacteria, directly or indirectly contributing to organic matter and soil carbon decomposition and nutrient cycling (Bödeker et al. 2016; Lindahl and Tunlid 2015; Talbot et al. 2008).
Despite the lack of trends mediated by trees on respiration and mycorrhizosphere production, we identified a significant decline in SOC stocks with increasing distance from trees (Table 1), from which we can infer a difference in C accumulation rates and/or turnover with distance from trees (Hypotheses 4a and 4b). A decline in SOC with increasing distance from the tree, or build-up of SOC closer to the tree, is likely a result of higher litter inputs (Fig. 7), or higher fungi:bacteria (Fig. 5b) ratio (and thus slower decomposition; Hypotheses 3 and 4), or a combination thereof. Respiration data (Fig. 2a) indicate that higher litter inputs close to the tree are not associated with higher rates of decomposition, which implies that, despite the apparent higher availability of C closer to trees, there is not a corresponding increase in microbial activity. This may reflect litter recalcitrance or slowed C turnover in ectomycorrhizal dominated soils potentially hampering early stage decomposition (Sterkenburg et al. 2018). Although we could not detect a trend in respiration per unit area, there was a clear increase in respiration per unit SOC with increasing distance from trees (Fig. 2b). This shows greater respiration per unit resource (specifically, here, carbon) available to heterotrophs further away from the tree (thus not supporting Hypothesis 4b). Greater respiration per unit SOC indicates higher potential for SOC loss if the carbon use efficiency (CUE) - the proportion of C assimilated by the microbial community relative to C lost through respiration - is low (Cotrufo et al. 2013; Sauvadet et al. 2018; Kallenbach et al. 2019). Furthermore, this suggests that the proportion of total respiration facilitated by the rhizosphere (roots and ECM) is larger further away from the tree (Fig. 4), consistent with declining SOC stocks (and leaf litter inputs) at increasing distances from trees. Larger relative influence of the rhizosphere suggests an increase in root and microbial activity further away from trees, with potentially low CUE, as there is no change in root and hyphal production along transects. Increased peripheral activity may result from competition between root and hyphal networks associated with neighbouring trees (Boddy 2000) and provides tantalising, although indirect, evidence for larger autotrophic contributions to respiration and potentially rhizosphere priming of the soil heterotrophic community (Fig. 6) (Fontaine et al. 2007; Wild et al. 2016). The current study does not, however, allow us to allocate the respiration signal to various C sources originating from many potential C pools within the ecosystem, and therefore caution must be applied when interpreting results.
Key soil processes, such as C turnover and storage, relate to the diversity of morphotypes (ECM or ERM, inferred here based on aboveground vegetation being ericaceous shrubs or Betula spp.), growth forms and exploration types of the mycorrhizal community (Cornelissen et al. 2001; Clemmensen et al. 2013, 2015; Hazard and Johnson 2018). Changes in soil microbial community composition along transects could be examined in more detail using appropriate sequencing techniques to identify microbial species present. Here we find that, although production rates of roots and hyphae remained consistently high up to 3 m from trees within the forest, there was a decline in the fungi:bacteria ratio, and therefore potential C storage (Bailey et al. 2002). The apparent discrepancy between hyphal production and fungi:bacteria ratio may be explained by an inability of the hyphal production assay to detect small changes in abundance detectable at a molecular level, or by variation in the abundance of bacteria or saprotrophic fungi, as the primers used are not ECM specific. This pattern may, in part, be caused by the dependence of saprotrophic fungi on C inputs in the form of birch tree litter, which decrease with increasing distance from trees (Fig. 7) and are more labile than understorey litter (Parker et al. 2018). Variation in standing litter is likely to cause spatial heterogeneity within the saprotrophic community not seen in mycorrhizal fungi communities, which receive plant photosynthates directly (Lindahl et al. 2007).
Our evidence for patchiness of SOC stocks in birch forests should be interpreted in the context of forest succession patterns, as varying SOC stocks and C accumulation in relation to individual trees may be linked to historical demographics and turnover (i.e. the forest seen today is merely a snapshot of a dynamic ecosystem (Callaghan et al. 2002; Van Bogaert et al. 2011). Without the on-going recruitment and deaths of trees, there might be net accumulation of SOC close to trees, and net loss of SOC further away from trees. However, with on-going turnover – tree death, and establishment of trees in forest gaps – the development, persistence and magnitude of spatial patterns in SOC stocks at contrasting distances from living trees will be related both to forest dynamics as well as soil organic matter turnover. Slow population turnover would lead to increasingly patchy SOC stocks driven by trees. However, establishment of trees in forest gaps on shorter time scales, could facilitate re-accumulation of lost SOC by new trees in forest gaps. In Fennoscandian Lapland, trees can be 200 years old (Tømmervik et al. 2009), but age varies significantly (86 ± 43 years (mean ± SD); Millar (1980)) with a high density of mountain birch seedlings/saplings (~1800 ha−1 of ≤1.5 m height and ≤ 15 mm basal stem diameter; B. Dick, pers. comm.). Decaying tree stumps are also evident and important as the influence of a single tree may be long lasting, given the microbial community it sustained whilst alive, and will influence future microbial communities after death (Clemmensen et al. 2013; Fernandez et al. 2016; Godbold et al. 2006; Varik et al. 2013). Furthermore, cyclical defoliating Epirrita moth outbreaks occur in this area on decadal timescales (Olsson et al. 2017; Tenow and Bylund 2000) causing tree loss or increased polycormy. Taken together, the timescales of forest dynamics may enable development of patchy SOC stocks related to individual trees, as found here. This further illustrates the complexity of soil C dynamics (Sulman et al. 2018) and emphasises the importance of long-term measurements and integration of biological traits with soil processes for global change modelling and predicting Arctic responses to climate change at plot and vegetation levels (Shaver et al. 2013; Wurzburger and Clemmensen 2018).
Based on our measurements, we suggest that the influence of an individual mountain birch tree within these forests extends at least 3 m from the main stem, far exceeding the average crown diameter (167 ± 35 cm; Table 3). This influence includes continued high production rates of fine roots and ectomycorrhizas to at least 3 m away from trees, with the ectomycorrhizal network likely to extend further beyond that of the root network (Anderson and Cairney 2007; Simard and Durall 2004). The spatial distribution and range of ectomycorrhizal hyphae depends on fine root distribution (Pickles et al. 2010) and mycorrhizal taxa (Agerer 2001), but mycelia have been demonstrated to extend up to 9.6 cm away from mycorrhizal root tips, depending on exploration types (Weigt et al. 2012). Although we lack direct evidence, the wide ranging influence and extent of the birch mycorrhizosphere and associated ectomycorrhizas in this area provides a tantalizing suggestion of the presence of a wood-wide-web and the potential for common mycelial networks (CMNs) (Beiler et al. 2010; Johnson 2015; Peter 2006; Selosse et al. 2006). The ability of ectomycorrhizas to colonise multiple host plants, and thereby create a cytoplasmic link through which nutrients (He et al. 2003; Selosse et al. 2006) and signalling molecules (Babikova et al. 2013) can be exchanged, has been well documented in the lab (Arnebrant et al. 1993; Finlay and Read 1986; Pickles et al. 2017). However, there are very few studies of CMNs in the field (Deslippe and Simard 2011; Simard et al. 1997) and, to our knowledge, none on mountain birch trees in the Arctic. Deslippe and Simard (2011) found transfer of C between conspecific pairs of dwarf birch (Betula nana L.) in the Alaskan Arctic and argued that the magnitude of this C transfer may alter competitive plant interactions in the arctic tundra. The implications of the potential presence of common mycelial networks in the Arctic, and in other regions, could be profound for forest health, C turnover and stocks, both above- and belowground.
Conclusions
Here we have focussed on the influence of mountain birch trees within forests on key soil processes such as respiration, SOC stocks, root and mycorrhizal production and microbial community abundance in an ecotone forest in sub-Arctic Sweden. We conclude that the direction, strength and magnitude of spatial trends within the forest floor in relation to proximal trees vary greatly. Our results show spatial variation in C accumulation rates in relation to nearest trees through changes in SOC stocks and litter fall. Contrastingly, we find no trend in respiration with distance from trees, likely mediated by an extensive root and ectomycorrhizal network of the birch trees, which efficiently exploit resources throughout the forest. This is an early indication of a ‘wood-wide-web’ in these forests. Further investigation is required, however, to understand the extent and significance of such a network. Recognising the interactions between the aboveground vegetation and belowground soil processes, and the soil C cycles that they mediate, is crucial in these globally important sub-Arctic ecosystems undergoing climate change.
Data Availability
All transect data including: respiration, root production, hyphae production, GPP, soil characteristics, vegetation cover, standing litter and molecular qPCR data will be added to the Dryad Data Repository upon acceptance.
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Acknowledgements
The research leading to these results has received funding from the European Union’s Horizon 2020 project INTERACT, under grant agreement No 730938. We extend our warm thanks to staff at the Abisko Scientific Research Station for hosting us and for supporting this work. We would also like to thank Ian Washbourne, Alyssa Parker, Ilona Kater and Gwen Lancashire for assistance in the field and processing of lab samples as well as Dr. Gaius R. Shaver for an extensive pre-submission review and Gerald Emmans for comments on an earlier version of this manuscript.
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Friggens, N.L., Aspray, T.J., Parker, T.C. et al. Spatial patterns in soil organic matter dynamics are shaped by mycorrhizosphere interactions in a treeline forest. Plant Soil 447, 521–535 (2020). https://doi.org/10.1007/s11104-019-04398-y
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DOI: https://doi.org/10.1007/s11104-019-04398-y