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Distribution pattern of aluminum in Panax notoginseng, a native medicinal plant adapted to acidic red soils

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Abstract

Aims

Panax notoginseng is normally cultivated in acidic red soil with high soluble Al, however, the distribution pattern of Al in P. notoginseng is unknown. The aim of this study is to investigate the Al distribution in different organs of P. notoginseng grown in different acid soil, in order to get a deeper insight into the Al tolerance mechanism in this species.

Methods

P. notoginseng was sampled from different sites and subjected to Al determination. Al tolerance and accumulation were investigated by exposing the seedlings to different Al concentrations in hydroponic solution. The expression of pectin methylesterase genes was determined by quantitative RT-PCR.

Results

The soil pH ranged from 4.68 to 6.13. The total Al concentration in soil was 76.7–168.5 g·kg−1 and active Al was 1.34–5.01 g·kg−1. Al concentration differed with organs of P. notoginseng, following the order: rootlet > rhizome > main root > leaf and stem. Furthermore, Al concentration in the cell wall, organelle and cytoplasmic supernatant of P. notoginseng was approximately 15:2:1 and most Al in the cell wall was bound to pectin component. Al treatment significantly increased the content of pectin and enhanced the expression of PME3, PME7, and PME40 of P. notoginseng root.

Conclusions

P. notoginseng was able to grow under high active Al condition and accumulated high Al in its underground parts. The pectin component in the cell wall was the major binding site of Al of P. notoginseng root.

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Abbreviations

DW:

Dry weight

FW:

Fresh weight

HC1:

Hemicellulose1

HC2:

Hemicellulose2

PME:

Pectin methylesterase

References

  • Anetta Z, Frankowski M (2017) Speciation analysis of aluminium in plant parts of betula pendula, and in soil. J Environ Sci 1–9

  • Blamey FPC, Asher CJ, Kerven GL, Edwards DG (1993) Factors affecting aluminium sorption by calcium pectate. Plant Soil 149:87–94

    Article  CAS  Google Scholar 

  • Blumenkrantz N, Asboe-Hansen G (1973) An improved method for the assay of hydroxylysine. Anal Biochem 56:10–15

    Article  CAS  PubMed  Google Scholar 

  • Chang YC, Yamamoto Y, Matsumoto H (1999) Accumulation of aluminium in the cell wall pectin in cultured tobacco (Nicotiana tabacum L.) cells treated with a combination of aluminium and iron. Plant Cell Environ 22(8):1009–1017

    Article  CAS  Google Scholar 

  • Chen T, Yan X, Liao X, Xiao XY, Huang ZC (2005) Subcellular distribution and compartmentalization of arsenic in Pteris vittata L. Science Bulletin 50:2843–2849

    Article  CAS  Google Scholar 

  • Chen YL, Wang HM, Hu W, Wang SS, Wang YH, Snider JL, Zhou ZG (2017) Combined elevated temperature and soil waterlogging stresses inhibit cell elongation by altering osmolyte composition of the developing cotton (Gossypium hirsutum L.) fiber. Plant Sci 256:196

    Article  CAS  PubMed  Google Scholar 

  • Cui XM (2017) Panax notoginseng cultivation. Science Press, Beijing (in Chinese)

    Google Scholar 

  • Eticha D, Stass A, Horst WJ (2005) Cell-wall pectin and its degree of methylation in the maize root-apex: significance for genotypic differences in aluminum resistance. Plant Cell Environ 28:1410–1420

    Article  CAS  Google Scholar 

  • Fan YZ, Fan LG, Yin HJ (2010) The al geochemical characteristics and resources potentiality of yunnan province. Yunnan. Geology 29:117–122

    Google Scholar 

  • Gan ZY, Peng JR, Nong YJ, Chen Q (2010) GFAAS determination of al in soil by microwave digestion. J Guangxi Acad Sci 26:287–288

    Google Scholar 

  • Gao HG, Yang SL, Zeng QW (2001) Soil and geological setting affecting the quality of pseudo-ginseng in Wenshan. Yunnan Geology 20:195–202

    Google Scholar 

  • Huang CM, Cui XM, Lan L, Chen WD, Wang CX, Yang XY, DH L, Yang Y (2015) Research on output and quality of Panax notoginseng and annual change characteristics of N, P and K nutrients of planting soil under stereo-cultivation. China J Chinese Materia Medica 40:2930–2936

    CAS  Google Scholar 

  • Kaul S, Koo H, Jenkins J, Copenhaver GP (2000) Analysis of the genome sequence of the flowering plant Arabidopsis Thaliana. Nature 408:796–815

    Article  CAS  Google Scholar 

  • Kochian LV, Piñeros MA, Liu J, Magalhaes JV (2015) Plant adaptation to acid soils: the molecular basis for crop aluminum resistance. Annu Rev Plant Biol 66:571

    Article  CAS  PubMed  Google Scholar 

  • Lai HY (2015) Subcellular distribution and chemical forms of cadmium in Impatiens walleriana in relation to its phytoextraction potential. Chemosphere 138:370–376

    Article  CAS  PubMed  Google Scholar 

  • Li H, Deng CB, Zhang S, Liang Y, Luo XG (2009) Total saponins of Panax notoginseng modulate the expression of caspases and attenuate apoptosis in rats following focal cerebral ischemia-reperfusion. J Ethnopharmacol 121:412

    Article  CAS  PubMed  Google Scholar 

  • Li Y, Chen X, Li Y, Liang Q, Shi F, Dou M, Ma L (2016) Correlation of agronomic character of Panax notoginseng in Guangxi with soil physical and chemical properties. J Chinese Medicinal Mater 39(9):1982–1988

    Google Scholar 

  • Liu L, Zhao AJ, Yang Y, Jin H, Cui XM, XH OU, Liu DH (2013) Comparative analysis of physical and chemical properties of Panax notoginseng replant soils in different intervals. Southwest China. Journal of Agricultural Sciences 26:1946–1952

    Google Scholar 

  • Ma JF (2007) Syndrome of aluminum toxicity and diversity of aluminum resistance in higher plants. Int Rev Cytol 264:225

    Article  CAS  PubMed  Google Scholar 

  • Ma SC (2012) Effects of aluminum on uptake, distribution and accumulation of fluorine in tea plants and its mechanism. Dissertation, Zhejiang University (in Chinese)

  • Maejima E, Watanabe T, Osaki M, Wagatsuma T (2014) Phosphorus deficiency enhances aluminum tolerance of rice (Oryza sativa) by changing the physicochemical characteristics of root plasma membranes and cell walls. J Plant Physiol 171(2):9–15

    Article  CAS  PubMed  Google Scholar 

  • Muñoz AH, Corona FG, Wrobel K, Soto GM, Wrobel K (2005) Subcellular distribution of aluminum, bismuth, cadmium, chromium, copper, iron, manganese, nickel, and lead in cultivated mushrooms (agaricus bisporus and pleurotus ostreatus). Biol Trace Elem Res 106(3):265–277

    Article  PubMed  Google Scholar 

  • Ng TB (2006) Pharmacological activity of sanchi ginseng (Panax notoginseng). J Pharm Pharmacol 58:1007

    Article  CAS  PubMed  Google Scholar 

  • Rohyadi A (2009) Neighboring plants alleviate aluminum toxicity on the external hyphae of gigaspora margarita. Microbiol Indonesia 3:42–46

    Article  Google Scholar 

  • Siecińska J, Nosalewicz A (2016) Aluminium toxicity to plants as influenced by the properties of the root growth environment affected by other co-stressors: a review. Rev Environ Contam Toxicol 243:1–26

    Google Scholar 

  • Su YJ, Liao W, Wang Y, Zhang Y, Luo Y, SG H (2014) Effects of tannic acid on active aluminum forms distribution in tea garden soils with different pH. Chin J Eco-Agric 22(1):22–30

    Article  CAS  Google Scholar 

  • Sun XT, Long GQ, Zhang GH, Meng ZG, Chen ZJ, Yang SC, Chen JW (2015) Properties of soil physical-chemistry and activities of soil enzymes in context of continuous cropping obstacles for Panax notoginseng. Ecol Environ Sci 24:409–417

    Google Scholar 

  • Tabuchi A, Matsumoto H (2001) Changes in cell-wall properties of wheat (Triticum aestivum) roots during aluminum-induced growth inhibition. Physiology Plant 112:353–358

    Article  CAS  Google Scholar 

  • Wang H, Li Y, Hou J, Huang J, Liang WH (2016) Nitrate reductase-mediated nitric oxide production alleviates al-induced inhibition of root elongation by regulating the ascorbate-glutathione cycle in soybean roots. Plant Soil 1:1–13

    Google Scholar 

  • Wang SY, Yuan SL, LT S, Lv AM, Zhou P, An Y (2017) Aluminum toxicity in alfalfa (Medicago sativa) is alleviated by exogenous foliar IAA inducing reduction of al accumulation in cell wall. Environ Exp Bot 139:1–13

    Article  CAS  Google Scholar 

  • Willats WGT, McCartney L, Mackie W, Knox JP (2001) Pectin: cell biology and prospects for functional analysis. Plant Mol Biol 47:9–27

    Article  CAS  PubMed  Google Scholar 

  • Xie ZL, Jin H, Wang JH, Wang XY (2010) Effects of interaction of calcium-aluminium on the adsorption capacity and active aluminium in tea garden soil under extraneous source organic matter. Journal of agro-environment. Science 29:293–298

    CAS  Google Scholar 

  • Yang JL, Li YY, Zhang YJ, Zhang SS, YR W, Wu P et al (2008) Cell wall polysaccharides are specifically involved in the exclusion of aluminum from the rice root apex. Plant Physiol 146:602–611

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yang Y, Wang W, Liu H, Zhi-Juan YE, Zhao ZQ, Geng MJ (2010) Effects of aluminum stress on root elongation of different aluminum tolerance wheat cultivars. Plant Nutr Fert Sci 16:584–590

    CAS  Google Scholar 

  • Yang JL, Zhu XF, Zheng C, Zhang YJ, Zheng SJ (2011) Genotypic differences in al resistance and the role of cell-wall pectin in al exclusion from the root apex in fagopyrum tataricum. Ann Bot 107(3):371–378

    Article  CAS  PubMed  Google Scholar 

  • Yang XY, Zeng ZH, Yan JY, Fan W, Bian HW, Zhu MY, Yang JL, Zheng SJ (2013) Association of specific pectin methylesterases with al-induced rootelongation inhibition in rice. Physiol Plant 148:502–511

    Article  CAS  PubMed  Google Scholar 

  • Yang Y, Tian MY, Guo LP, Liu DH, Qu Y, Cui XM (2017) Effect of different cleaning processes on saponins’ loss rate and total ash clearance rate of Notoginseng and heavy metal health risk assessment J Food Process Preserv e13409. https://doi.org/10.1111/jfpp.13409

  • Yin C, Peng L, Sun L (1999) The characteristics of aluminium contents in compositae plants for medicine in yunnan. J Environ Health 02:334–3441

    Google Scholar 

  • Yoshikawa M, Morikawa T, Kashima Y, Ninomiya K, Matsuda H (2003) Structures of new dammarane-type triterpene saponins from the flower buds of and hepatoprotective effects of principal ginseng saponins . J Nat Prod 66(7):922–927

  • Zeng YW, Wang LX, Du J, Yang SM, Wang YC, Li QW, Sun ZH, Pu XY, Du W (2009) Correlation of mineral elements between milled and brown rice and soils in yunnan studied by icpaes. Spectrosc Spectr Anal 29(5):1413–1417

    CAS  Google Scholar 

  • Zhang GS, Li ZJ, Ai R, Jin LH (2011) Impacts of native and exotic vegetation types on a cambisols properties in the mid-yunnan plateau. Chinese J Soil Sci 42(4):852–858

    CAS  Google Scholar 

  • Zhang Q, Bao YQ, Tan XY (2015) Functional analysis of Arabidopsis Thaliana pectin methylesterase gene PME17 in immunity against pseudomonas syringae pv. Tomato DC3000. Plant Physiol J 51(7):1061–1066

    CAS  Google Scholar 

  • Zhao Y, Wu J, Shang D, Ning J, Zhai Y, Sheng X, Ding H (2015) Subcellular distribution and chemical forms of cadmium in the edible seaweed, Porphyra yezoensis. Food Chem 168:48–54

    Article  CAS  PubMed  Google Scholar 

  • Zhou Y, XY X, Chen LQ, Yang JL, Zheng SJ (2012) Nitric oxide exacerbates al-induced inhibition of root elongation in rice bean by affecting cell wall and plasma membrane properties. Phytochemistry 76(12):46–51

    Article  CAS  PubMed  Google Scholar 

  • Zhu ML, Li TF, Xia ZY, Zhang KQ (2003) Analyzing on the ecology factor of the soil infected by tobacco root knot nematodes in yunnan. Acta Tabacaria Sinica 9:30–34

    Google Scholar 

Download references

Acknowledgements

This work was supported by the Natural Science Foundation of China (No. 31201677, 81460580 and 31660595), China Postdoctoral Science Foundation (No. 167358).

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Authors and Affiliations

  1. Yunnan Provincial Key Laboratory of Panax notoginseng, Key Laboratory of Panax notoginseng Resources Sustainable Development and Utilization of State Administration of Traditional Chinese Medicine, Kunming Key Laboratory of Sustainable Development and Utilization of Famous-Region Drug, Faculty of Life Science and Technology, Kunming University of Science and Technology, No.727 South Jingming Rd., Chenggong District, Kunming, 650500, China

    Yang Ye, Dai Chunyan, Qu Yuan, Yang Xiaoyan, Chen Qi, Liu Diqiu, Wang Chengxiao & Cui Xiuming

  2. Chinese Medica Resources Center, China Academy of Chinese Medicinal Sciences, Beijing, 100700, China

    Guo Lanping

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  1. Yang Ye
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  2. Dai Chunyan
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  3. Guo Lanping
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  4. Qu Yuan
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  5. Yang Xiaoyan
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  9. Cui Xiuming
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Correspondence to Wang Chengxiao or Cui Xiuming.

Additional information

Responsible Editor: Jian Feng Ma.

Electronic supplementary material

Supplementary Fig. 1

Plant morphology of P. notoginseng (GIF 277 kb)

High resolution image (TIFF 438 kb)

Supplementary Table 1

(DOC 19 kb)

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Ye, Y., Chunyan, D., Lanping, G. et al. Distribution pattern of aluminum in Panax notoginseng, a native medicinal plant adapted to acidic red soils. Plant Soil 423, 375–384 (2018). https://doi.org/10.1007/s11104-017-3510-8

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