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Effects of a bacterial consortium from acid mine drainage on cadmium phytoextraction and indigenous soil microbial community

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Abstract

Background and aims

A major concern in developing microbially-assisted phytoextraction (MAP) is that the effects of introduced microbes on indigenous soil microbial community are profound and irreversible. To date, however, the microbial properties of soils subjected to MAP remain poorly understood. Therefore, we explored the effects of inoculation with a bacterial consortium enriched from acid mine drainage on not only the cadmium (Cd) phytoextraction efficiency of Averrhoa carambola but also the microbial properties of the Cd-contaminated soil.

Methods

We conducted a field experiment and characterized the microbial community in the contaminated soil using next generation sequencing technology (Illumina MiSeq).

Results

The bacterial inoculation increased the Cd concentration in A. carambola shoot tissues by 20%–65%, leading to a relatively high Cd removal efficiency (4.63% annually). Meanwhile, there were no significant differences between the treatments in soil bacterial diversity and community composition one year after the initiation of the bacterial inoculation treatment. The most abundant genera of the introduced bacteria were found to either disappear from, or be present in similar relative abundance, in the soils of the different treatments, except Sulfobacillus.

Conclusions

Collectively, our results provide evidence that MAP could be practiced with minor effects on indigenous soil microbial community.

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Acknowledgements

We thank Prof. Alan Baker of the University of Melbourne for his insightful comments that have helped to improve the quality of the manuscript. This work was funded by the National Natural Science Foundation of China (Nos. 31100372 and 41471257), the Pearl River Nova Program of Guangzhou (No. 2014 J2200100), the Fok Ying Tong Education Foundation (No. 142025), the Guangdong Provincial Natural Science Foundation (No. 10451027501005629), and the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20110171120042).

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resource, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People’s Republic of China

    Jin-tian Li, Zhi-wei Liang, Pu Jia, Jun Liu, Yi-juan Xu, Yong-jian Chen, Hao-yue Shu, Jia-liang Kuang, Bin Liao & Wen-sheng Shu

  2. Biological Sciences, Scarborough & Ecology and Evolutionary Biology, University of Toronto, 1265 Military Trail, Toronto, ON, M1C 1A4, Canada

    Jin-tian Li

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  1. Jin-tian Li
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Correspondence to Wen-sheng Shu.

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Responsible Editor: Fangjie Zhao.

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Li, Jt., Liang, Zw., Jia, P. et al. Effects of a bacterial consortium from acid mine drainage on cadmium phytoextraction and indigenous soil microbial community. Plant Soil 415, 347–358 (2017). https://doi.org/10.1007/s11104-016-3170-0

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