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CBM20CP, a novel functional protein of starch metabolism in green algae

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Abstract

Ostreococcus tauri is a picoalga that contains a small and compact genome, which resembles that of higher plants in the multiplicity of enzymes involved in starch synthesis (ADP-glucose pyrophosphorylase, ADPGlc PPase; granule bound starch synthase, GBSS; starch synthases, SSI, SSII, SSIII; and starch branching enzyme, SBE, between others), except starch synthase IV (SSIV). Although its genome is fully sequenced, there are still many genes and proteins to which no function was assigned. Here, we identify the OT_ostta06g01880 gene that encodes CBM20CP, a plastidial protein which contains a central carbohydrate binding domain of the CBM20 family, and a coiled coil domain at the C-terminus that lacks catalytic activity. We demonstrate that CBM20CP has the ability to bind starch, amylose and amylopectin with different affinities. Furthermore, this protein interacts with OsttaSSIII-B, increasing its binding to starch granules, its catalytic efficiency and promoting granule growth. The results allow us to postulate a functional role for CBM20CP in starch metabolism in green algae.

Key message

CBM20CP, a plastidial protein that has a modular structure but lacks catalytic activity, regulates the synthesis of starch in Ostreococcus tauri.

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Acknowledgements

We thank Dr. Olivier Vallon (Institut de Biologie Physico-Chimique CNRS/Sorbonne Université, Paris, France), Dr. Marianne Tardif (Atomic Energy and Alternative Energies Commission, Gif-sur-Yvette, France) and Dr. Laurent Cournac (Institute of Research for Development, Marseille, France) for their prompt support with PredAlgo tool. NH was a postdoctoral fellow from CONICET and MBV is a doctoral fellow from the same institution. JB, DFGC and MVB are research scientists from CONICET. This work was supported in part by grants from ANPCyT (PICT 2018 01440 to MVB).

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  1. Nicolas Hedin and Maria B. Velazquez have contributed equally to this study.

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  1. CEFOBI – CONICET. Centro de Estudios Fotosintéticos y Bioquímicos - Consejo Nacional de Investigaciones Científicas y Técnicas. Facultad de Ciencias Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, Santa Fe, Argentina

    Nicolas Hedin, Maria B. Velazquez, Julieta Barchiesi, Diego F. Gomez-Casati & Maria V. Busi

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  1. Nicolas Hedin
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  2. Maria B. Velazquez
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JB, DFGC, MVB conceived, designed and analyzed the experiments. NH, MBV performed the experiments and analyzed the results. JB, DFGC and MVB wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Maria V. Busi.

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Hedin, N., Velazquez, M.B., Barchiesi, J. et al. CBM20CP, a novel functional protein of starch metabolism in green algae. Plant Mol Biol 108, 363–378 (2022). https://doi.org/10.1007/s11103-021-01190-4

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