Abstract
Key message
Genome-wide targets of Actinidia chinensis SVP2 confirm roles in ABA- and dehydration-mediated growth repression and reveal a conservation in mechanism of action between SVP genes of taxonomically distant Arabidopsis and a woody perennial kiwifruit.
Abstract
The molecular mechanisms underlying growth and dormancy in woody perennials are largely unknown. In Arabidopsis, the MADS-box transcription factor SHORT VEGETATIVE PHASE (SVP) plays a key role in the progression from vegetative to floral development, and in woody perennials SVP-like genes are also proposed to be involved in controlling dormancy. During kiwifruit development SVP2 has a role in growth inhibition, with high-chill kiwifruit Actinidia deliciosa transgenic lines overexpressing SVP2 showing suppressed bud outgrowth. Transcriptomic analyses of these plants suggests that SVP2 mimics the well-documented abscisic acid (ABA) effect on the plant dehydration response. To corroborate the growth inhibition role of SVP2 in kiwifruit development at the molecular level, we analysed the genome-wide direct targets of SVP2 using chromatin immunoprecipitation followed by high-throughput sequencing in kiwifruit A. chinensis. SVP2 was found to bind to at least 297 target sites in the kiwifruit genome, and potentially modulates 252 genes that function in a range of biological processes, especially those involved in repressing meristem activity and ABA-mediated dehydration pathways. In addition, our ChIP-seq analysis reveals remarkable conservation in mechanism of action between SVP genes of taxonomically distant plant species.
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Acknowledgements
The authors wish to thank Cecilia Deng and Lindy Guo for assistance in bioinformatics and statistical analysis, Monica Dragulescu and Wade Wadasinghe for maintenance of plants in the glasshouse, Roger Hellens and Jo Putterill for advice on the project design and progress, and Zac Hanley and Ann Gunson for critical reading of the manuscript. This work was funded by the New Zealand Ministry of Business, Innovation and Employment, contract C10 × 0816 MeriNET, and was supported by funding from The New Zealand Institute for Plant and Food Research Limited (Core and KRIP).
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RW and EV-G designed the study; RW and TW performed the experimental work; RW, BAW and SJT performed bioinformatics analyses; RW interpreted the data and wrote the manuscript; EV-G, RCM and ACA conceived the project and revised the manuscript.
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Wu, R., Wang, T., Warren, B.A.W. et al. Kiwifruit SVP2 controls developmental and drought-stress pathways. Plant Mol Biol 96, 233–244 (2018). https://doi.org/10.1007/s11103-017-0688-3
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DOI: https://doi.org/10.1007/s11103-017-0688-3