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OsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factors

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Abstract

High salinity causes remarkable losses in rice productivity worldwide mainly because it inhibits growth and reduces grain yield. To cope with environmental changes, plants evolved several adaptive mechanisms, which involve the regulation of many stress-responsive genes. Among these, we have chosen OsRMC to study its transcriptional regulation in rice seedlings subjected to high salinity. Its transcription was highly induced by salt treatment and showed a stress-dose-dependent pattern. OsRMC encodes a receptor-like kinase described as a negative regulator of salt stress responses in rice. To investigate how OsRMC is regulated in response to high salinity, a salt-induced rice cDNA expression library was constructed and subsequently screened using the yeast one-hybrid system and the OsRMC promoter as bait. Thereby, two transcription factors (TFs), OsEREBP1 and OsEREBP2, belonging to the AP2/ERF family were identified. Both TFs were shown to bind to the same GCC-like DNA motif in OsRMC promoter and to negatively regulate its gene expression. The identified TFs were characterized regarding their gene expression under different abiotic stress conditions. This study revealed that OsEREBP1 transcript level is not significantly affected by salt, ABA or severe cold (5 °C) and is only slightly regulated by drought and moderate cold. On the other hand, the OsEREBP2 transcript level increased after cold, ABA, drought and high salinity treatments, indicating that OsEREBP2 may play a central role mediating the response to different abiotic stresses. Gene expression analysis in rice varieties with contrasting salt tolerance further suggests that OsEREBP2 is involved in salt stress response in rice.

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Acknowledgments

We are grateful to Prof. Dorothea Bartels for her critical reading of the manuscript and Dr. Pieter B. F. Ouwerkerk for his helpful advices regarding the yeast one-hybrid system. This work was supported by Fundação para a Ciência e a Tecnologia (FCT) through national funds allocated to research projects [POCI/BIA-BCM/56063/2004 and PTDC/BIA-BCM/099836/2008] and PhD scholarships [SFRH/BD/31011/2006 to TS, SFRH/BD/29258/2006 to DF, SFRH/BD/74946/2010 to AC, SFRH/BD/65229/2009 to DA, SFRH/BPD/34943/2007 to TL]. NS and IA were supported by Programa Ciência 2007, financed by POPH (QREN). AS and BCM work was supported by funding from Programa Euroinvestigación 2008 [EUI2008-03612].

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Authors and Affiliations

  1. Genomics of Plant Stress Laboratory, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da Republica, 2780-157, Oeiras, Portugal

    Tânia S. Serra, Duarte D. Figueiredo, André M. Cordeiro, Diego M. Almeida, Tiago Lourenço, Isabel A. Abreu, M. Margarida Oliveira & Nelson J. M. Saibo

  2. Instituto de Biologia Experimental e Tecnológica, Av. da Republica, 2780-157, Oeiras, Portugal

    Tânia S. Serra, Duarte D. Figueiredo, André M. Cordeiro, Diego M. Almeida, Tiago Lourenço, Isabel A. Abreu, M. Margarida Oliveira & Nelson J. M. Saibo

  3. Laboratory of Computational Biology, Estación Experimental de Aula Dei/CSIC, Av. Montañana 1005, Zaragoza, Spain

    Alvaro Sebastián & Bruno Contreras-Moreira

  4. Yeast Stress Laboratory, Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156, Oeiras, Portugal

    Lisete Fernandes

  5. Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa, Av. D. João II, lote 4.69.01, 1900-096, Lisbon, Portugal

    Lisete Fernandes

  6. Fundación ARAID, Paseo María Agustín 36, Zaragoza, Spain

    Bruno Contreras-Moreira

Authors
  1. Tânia S. Serra
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  2. Duarte D. Figueiredo
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  3. André M. Cordeiro
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  4. Diego M. Almeida
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  5. Tiago Lourenço
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  6. Isabel A. Abreu
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  7. Alvaro Sebastián
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  8. Lisete Fernandes
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  9. Bruno Contreras-Moreira
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  10. M. Margarida Oliveira
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  11. Nelson J. M. Saibo
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Corresponding author

Correspondence to Nelson J. M. Saibo.

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Serra, T.S., Figueiredo, D.D., Cordeiro, A.M. et al. OsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factors. Plant Mol Biol 82, 439–455 (2013). https://doi.org/10.1007/s11103-013-0073-9

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  • DOI: https://doi.org/10.1007/s11103-013-0073-9

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