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Development and Validation of an HPLC-UV Method for Anilocaine Determination in Blood Plasma

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Pharmaceutical Chemistry Journal Aims and scope

A method for anilocaine determination in rat blood plasma using HPLC with UV-detection was developed. Samples were prepared by liquid—liquid extraction with 1-BuOH—hexane (2:98, v/v) followed by stripping with aqueous formic acid (0.1%). Chromatographic analysis used a Nucleodur HILIC column eluted in isocratic mode by a mobile phase of MeCN and aqueous ammonium formate (25 mM) (85:15) and UV detection at 205 nm. The method was validated for selectivity, carry-over, calibration curve linearity, precision, accuracy, limit of quantitation, dilution integrity, and stability. The analytical range was 25 – 1,000 ng/mL. The proposed method could be applied to pharmacokinetic studies of anilocaine preparations.

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Authors and Affiliations

  1. V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow, 123182, Russia

    V. A. Ryzhikova, V. Yu. Belov & V. I. Sevast’yanov

  2. Institute of Biomedical Research and Technology, Moscow, 123557, Russia

    S. V. Kursakov, V. Yu. Belov & V. I. Sevast’yanov

Authors
  1. V. A. Ryzhikova
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  2. S. V. Kursakov
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  3. V. Yu. Belov
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  4. V. I. Sevast’yanov
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Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 52, No. 2, pp. 54 – 58, February, 2018.

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Ryzhikova, V.A., Kursakov, S.V., Belov, V.Y. et al. Development and Validation of an HPLC-UV Method for Anilocaine Determination in Blood Plasma. Pharm Chem J 52, 166–170 (2018). https://doi.org/10.1007/s11094-018-1784-7

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  • DOI: https://doi.org/10.1007/s11094-018-1784-7

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