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Development of an HPLC-UV Method for Quantitative Determination of Acetylsalicylic Acid and Its Main Metabolite

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Pharmaceutical Chemistry Journal Aims and scope

An HPLC-UV method for determination of acetylsalicylic acid and its main metabolite, salicylic acid, in a model solution and in rabbit blood plasma was developed. Plasma samples were prepared by salting out. Chromatographic analysis was performed in isocratic mode over a Hypersil BDS C18 column using mobile phase MeCN—H2O (pH 2.5, 30:70) with detection at 230 nm. The limit of quantitation for acetylsalicylic and salicylic acids in the model solution was 0.05 μg/mL; in blood plasma, 0.2 μg/mL. The developed method was applied to the development of new acetylsalicylic-acid dosage forms based on biocompatible polymer carriers, including pharmacokinetic studies after i.m. implantation.

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Acknowledgments

The work was financially supported by the Russian Foundation for Basic Research (Project No. 13-02-12215).

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Authors and Affiliations

  1. Institute of Biomedical Research and Technology, Moscow, 123557, Russia

    V. Yu. Belov, S. V. Kursakov & V. I. Sevast’yanov

  2. V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow, 123182, Russia

    V. I. Sevast’yanov

  3. Institute of Laser and Information Technologies, Russian Academy of Sciences, Shatura, Moscow Oblast, 140700, Russia

    E. N. Antonov, S. É. Bogorodskii & V. K. Popov

Authors
  1. V. Yu. Belov
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  2. S. V. Kursakov
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  3. V. I. Sevast’yanov
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  4. E. N. Antonov
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  5. S. É. Bogorodskii
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  6. V. K. Popov
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Corresponding author

Correspondence to V. K. Popov.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 52, No. 2, pp. 40 – 44, February, 2018.

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Belov, V.Y., Kursakov, S.V., Sevast’yanov, V.I. et al. Development of an HPLC-UV Method for Quantitative Determination of Acetylsalicylic Acid and Its Main Metabolite. Pharm Chem J 52, 151–155 (2018). https://doi.org/10.1007/s11094-018-1781-x

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  • DOI: https://doi.org/10.1007/s11094-018-1781-x

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