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Catuaba (Trichilia catigua) Prevents Against Oxidative Damage Induced by In Vitro Ischemia–Reperfusion in Rat Hippocampal Slices

Neurochemical Research volume 37, pages 2826–2835 (2012)Cite this article

Abstract

Oxidative stress is implicated in brain damage associated with ischemia–reperfusion. Natural antioxidants found in some plants used in folk medicine have been indicated as potential neuroprotective agents. Here we investigated whether Trichilia catigua, a traditional Brazilian herbal medicine alleged to exhibit a variety of neuropharmacological properties (antidepressant, anti-neurasthenic, anti-inflammatory etc.), could have neuroprotective properties in rat hippocampal slices subjected to 2 h oxygen and glucose deprivation (OGD) followed by 1 h reperfusion. Ischemia–reperfusion (I/R) significantly decreased mitochondrial viability, increased dichlorofluorescein oxidation above control both in the incubation medium and slices homogenates, increased lactate dehydrogenase into the incubation medium and decreased non-protein thiols. T. catigua (40–100 μg/mL) protected slices from the deleterious effects of OGD when present before OGD and during the reperfusion periods. Oxidative stress in the medium was also determined under different conditions and the results demonstrated that T. catigua could not protect slices from I/R when it was added to the medium after ischemic insult. Although the translation to a real in vivo situation of I/R is difficult to be done, the results indicated that T. catigua should be used as preventive and not as a curative agent against brain damage.

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Acknowledgments

JPK would like to thanks specially CNPq-TWAS for financial support. JPK is a beneficiary of the TWAS-CNPq postgraduate (Doctoral) fellowship. Work supported by CNPq, CAPES, FAPERGS, FAPERGS-PRONEX-CNPq, VITAE Fundation, Rede Brasileira de Neurociências (IBNET-FINEP), FINEP-CTINFRA and INCT for excitotoxicity and neuroprotection-CNPQ.

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The authors declare no conflict of interest with any person or other organization.

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Authors and Affiliations

  1. Departamento de Química, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, CEP 97105-900, Brazil

    Jean Paul Kamdem, Ige Joseph Kade & João Batista Teixeira Rocha

  2. Department of Biochemistry, Federal University of Technology, Akure, PMB 704, Ondo State, Nigeria

    Ige Joseph Kade

  3. Universidade Federal do Pampa, Campus Caçapava do Sul, Caçapava do Sul, RS, CEP 96570-000, Brazil

    Caroline Wagner

  4. Departamento de Farmácia Industrial, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Aline Augusti Boligon & Margareth Linde Athayde

  5. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Jean Paul Kamdem & Diogo Onofre Souza

  6. Universidade Federal do Pampa, UNIPAMPA, Uruguaina, RS 97500-970, Brazil

    Emily Pansera Waczuk

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  1. Jean Paul Kamdem
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Kamdem, J.P., Waczuk, E.P., Kade, I.J. et al. Catuaba (Trichilia catigua) Prevents Against Oxidative Damage Induced by In Vitro Ischemia–Reperfusion in Rat Hippocampal Slices. Neurochem Res 37, 2826–2835 (2012). https://doi.org/10.1007/s11064-012-0876-0

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  • DOI: https://doi.org/10.1007/s11064-012-0876-0

Keywords

  • Trichilia catigua
  • Hippocampus
  • Neuroprotection
  • Antioxidant
  • Oxidative stress