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Neuroprotective Effect of Sesame Seed Oil in 6-Hydroxydopamine Induced Neurotoxicity in Mice Model: Cellular, Biochemical and Neurochemical Evidence

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Abstract

Natural antioxidants have shown a remarkable reduction in oxidative stress due to excess formation of reactive oxygen species by enhancing antioxidant mechanism in the neurodegenerative disorders. Sesame seed oil (SO) is one of the most important edible oil in India as well as in Asian countries and has potent antioxidant properties thus the present study evaluated the neuroprotective effect of SO by using 6-Hydroxydopamine (6-OHDA)-induced Parkinson’s disease model in mice. The mice were fed an SO mix diet for 15 days and then 6-OHDA was injected into the right striatum of mice brain. Three weeks after 6-OHDA infusion, mice were sacrificed and the striatum was removed. The neuroprotective role of SO on the activities of antioxidant and non-antioxidant enzymes such as glutathione reductase (GR), glutathione-S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT) and content of glutathione (GSH) and thiobarbituric acid reactive substance (TBARS) were studied in the striatum. The activities of all the above-mentioned enzymes decreased significantly in 6-OHDA group (Lesioned) when compared with Sham. The pretreatment of SO on antioxidant mechanism and dopamine level in the brain had shown some significant improvement in Lesion+SO (L+SO) group when compared with Lesioned group. However, NADPH oxidase subunit, Nox2 and inflammatory stimulator Cox2 expression was increased as well as antioxidant MnSOD level was decreased in Lesioned group while SO showed the inhibitory effect on the activation of Nox2 and Cox2 and restored MnSOD expression in L+SO group. Increased tyrosine hydroxylase (TH) expression in substantia nigra as well as dopamine and its metabolite DOPAC level in L+SO group also support our findings that SO may inhibit activation of NADPH oxidase dependent inflammatory mechanism due to 6-OHDA induced neurotoxicity in mice.

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Acknowledgments

Authors are thankful to CSIR, Ministry of Science and Technology, New Delhi, Govt. of India for providing authentic sesame seed as a gift sample to carry out this research work. This work was supported by women young scientist award (Department of Science and Technology (DST), New Delhi, Govt. of India) to DPK. We greatly acknowledge Ms Sylvia Megyardi, Oral Biology and Anatomy, GHSU, Augusta, GA, USA for reviewing and editing this manuscript.

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There is no conflict of interest with any person or other organization.

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Author notes

  1. Saif Ahmad

    Present address: Department of Ophthalmology, School of Medicine, Georgia Health Sciences University (GHSU), Augusta, GA, 30912, USA

  2. M. Badruzzaman Khan

    Present address: Center for Molecular Chaperone/Radiobiology and Cancer Virology, School of Medicine, Georgia Health Sciences University (GHSU), Augusta, GA, 30912, USA

  3. Kanchan Bhatia

    Present address: Experimental Medicine, School of Medicine, Georgia Health Sciences University (GHSU), Augusta, GA, 30912, USA

  4. Rizwanul Haque

    Present address: Department of Microbiology and Immunology, C6749 Penn State College of Medicine, 500 University Drive, Hershey, PA, 17033, USA

  5. Inayat Saleem Fazili

    Present address: Center of Animal Biotechnology, Sher-i-Kashmir University of Agricultural Sciences and Technology, Shuhama (Alusteng), Srinagar, 190 006, India

  6. Arshad Jamal

    Present address: Biological Science Department, College of Science and Arts Rabigh, King Abdulaziz University, Jeddah, Saudi Arabia

  7. Deepshikha Pande Katare

    Present address: Amity Institute of Biotechnology, Amity University, Sector-125, Noida, Uttar Pradesh, 201303, India

  8. M. Nasrul Hoda

    Present address: Department of Neurology, School of Medicine, Georgia Health Sciences University (GHSU), Augusta, GA, 30912, USA

Authors and Affiliations

  1. Department of Biotechnology, Jamia Hamdard (Hamdard University), New Delhi, 110 062, India

    Saif Ahmad, Inayat Saleem Fazili, Arshad Jamal & Deepshikha Pande Katare

  2. Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), New Delhi, 110062, India

    M. Badruzzaman Khan, M. Nasrul Hoda, Kanchan Bhatia & Rizwanul Haque

  3. Council of Scientific and Industrial Research(CSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India

    Jafar Salamt Khan

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  1. Saif Ahmad
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Ahmad, S., Khan, M.B., Hoda, M.N. et al. Neuroprotective Effect of Sesame Seed Oil in 6-Hydroxydopamine Induced Neurotoxicity in Mice Model: Cellular, Biochemical and Neurochemical Evidence. Neurochem Res 37, 516–526 (2012). https://doi.org/10.1007/s11064-011-0638-4

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