In experiments on Wistar rats, we found that, within an early period (2 to 4 h) after injection of bacterial lypopolysaccharide, LPS (0.1 mg/kg, i.p.), the latency of generalized seizures induced by injection of benzylpenicillin (sodium salt; 3.0 million IU/kg, i.p.) became significantly shorter, while the severity of seizure manifestations was higher than in the control group. Within this period, the power of oscillations of the delta and alpha-frequency ranges increased in the frontal cortex and hippocampal structures; fast ECoG components (beta and gamma rhythms) were suppressed, and the power of the theta activity decreased. In the hippocampal structures, these changes were more clearly pronounced, as compared with the neocortex. Within a later period of the action of LPS (12 to 18 h from the moment of injection), the latency of penicillin-induced seizures significantly exceeded the control value, and the severity of such seizures was lower. Under such conditions, we observed a smaller power of the synchronized activity of delta and alpha frequencies combined with intensification of the theta activity (most clearly pronounced in the hippocampal structures), and also an increase in the power of “desynchronized” rhythms (beta and gamma oscillations) in the cortex and hippocampus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
L. S. Godlevsky and O. M. Nenova, “Neuroimmunological mechanisms underlying the control of excitability of the brain,” Dosyagnennya Biol. Med., 8, No. 1, 75–92 (2006).
V. I. Kresyun, E. V. Kobole, I. V. Smirnov, et al., “Effects of interferon-2α on convulsive manifestations in corazol-induced kindling,” Byull. Éksp. Biol. Med., 114, No. 7, 11–14 (2007).
A. A. Shandra, L. S. Godlevsky, R. S. Vastyanov, et al., “The role of TNF-α in amygdala kindled rats,” Neurosci. Res., 42, No. 1, 147–153 (2002).
S. Kapadia, J. Lee, G. Torre-Amione, et al., “Tumor necrosis factor-α gene and protein expression in adult feline myocardium after endotoxin administration,” J. Clin. Invest., 96, No. 3, 1042–1052 (1995).
J. G. Heida, G. C. Teskey, and Q. J. Pittman, “Febrile convulsions induced by the combination of lipopolysaccharide and low-dose kainic acid enhance seizure susceptibility, not epileptogenesis, in rats,” Epilepsia, 46, No. 12, 1898–1905 (2005).
J. D. Morrow and M. R. Opp, “Diurnal variation of lipopolysaccharide-induced alterations in sleep and body temperature of interleukin-6-deficient mice,” Brain, Behav., Immun., 19, No. 1, 40–51 (2005).
J. Mullington, C. Korth, D. M. Hermann, et al., “Dose-dependent effects of endotoxin on human sleep,” Am. J. Physiol. Regul. Integr. Comp. Physiol., 278, No. 4, 947–955 (2000).
T. Schiffelholz and M. Lancel, “Sleep changes induced by lipopolysaccharide in the rat are influenced by age,” Am. J. Physiol. Regul. Integr. Comp. Physiol., 280, No. 2, 398–403 (2001).
I. V. Smirnov, “Effect of bacterial lypopolysaccharide on generalized clonico-tonic and clonic seizures in rats,” Dosyagnennya Biol. Med., 11, No. 1, 26–31 (2008).
E. S. Akarsu, S. Ozdayi, E. Algan, and F. Ulupinar, “The neuronal excitability time-dependently changes after lipopolysaccharide administration in mice: possible role of cyclooxygenase-2 induction,” Epilepsy Res., 71, Nos. 2/3, 181–187 (2006).
G. Paxinos and Ch. Watson, The Rat Brain in Stereotaxic Coordinates, Academic Press, Sydney (1982).
L. S. Godlevskii (L.S. Godlevsky), E. M. Barnyak, A. M. Matsko, et al., “Effects of transcranial magnetostimulation on the epileptiform activity in rats with electrostimulation-induced kindling,” Neurophysiology, 33, No. 2, 114–117 (2001).
C. Mrangoz and F. Bagrici, “Effects of L-arginine on penicillin-induced epileptiform activity in rats,” Jpn. J. Pharmacol., 86, No. 3, 297–301 (2001).
L. S. Godlevsky, E. V. Kobolev, and I. V. Smirnov, Stimulation of the Brain: Mechanisms of Cessation of Seizure Activity [in Russian], Neptun-Tekhnologiya, Odessa (2006).
A. A. Shandra, L. S. Godlevsky, and A. I. Brusentsov, Kindling and Epileptic Activity [in Russian], Astropring, Odessa (1999).
D. M. Woodbury, “Convulsant drug: Mechanisms of action,” in: Antiepileptic Drugs: Mechanism of Action, Raven Press, New York (1980), pp. 249–303.
M. Lancel, J. Cronlein, P. Muller-Preuss, and F. Holsboer, “Lipopolysaccharide increases EEG delta activity within non-REM sleep and disrupts sleep continuity in rats,” Am. J. Physiol. Regul. Integr. Comp. Physiol., 268, No. 5, 1310–1318 (1995).
S. Kamei, T. Sakai, M. Matsuura, et al., “Alterations of quantitative EEG and mini-mental state examination in interferon-α-treated hepatitis C,” Eur. Neurol., 48, No. 1, 102–107 (2002).
R. G. Biniaurishvili, A. M. Vein, B. G. Gafurov, and A. R. Rakhimdzhanov, Epilepsy and Functional State of the Brain [in Russian], Meditsina, Moscow (1985).
F. N. Serkov and V. N. Kazakov, Neurophysiology of the Thalamus [in Russian], Naukova Dumka, Kyiv (1980).
M. N. Shouse, J. M. Siegel, M. F. Wu, et al., “Mechanisms of seizure suppression during rapid-eye-movement (REM) sleep in cats,” Brain Res., 505, Nos. 1/2, 271–282 (1989).
Author information
Authors and Affiliations
Corresponding author
Additional information
Neirofiziologiya/Neurophysiology, Vol. 40, No. 3, pp. 236–241, May–June, 2008.
Rights and permissions
About this article
Cite this article
Godlevsky, L.S., Kobolev, E.V. & Smirnov, I.V. Effect of bacterial lypopolysaccharide on penicillin-induced seizure activity in rats. Neurophysiology 40, 199–203 (2008). https://doi.org/10.1007/s11062-008-9035-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11062-008-9035-2