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Synthesis, characterization and mechanistic insights of mycogenic iron oxide nanoparticles

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Abstract

In the present study, extracellular synthesis of iron oxide nanoparticles (IONPs) was achieved using Aspergillus japonicus isolate AJP01. The isolate demonstrated its ability to hydrolyze the precursor salt solution, a mixture of iron cyanide complexes, under ambient conditions. Hydrolysis of these complexes released ferric and ferrous ions, which underwent protein-mediated coprecipitation and controlled nucleation resulting in the formation of IONPs. Transmission electron microscopy, selected area electron diffraction pattern, energy dispersive spectroscopy and grazing incidence X-ray diffraction analysis confirmed the mycosynthesis of IONPs. The synthesized particles were cubic in shape with a size range of 60–70 nm with crystal structure corresponding to magnetite. Scanning electron microscopy analysis revealed the absence of IONPs on fungal biomass surface, indicating the extracellular nature of synthesis. Fourier transform infrared spectroscopy confirmed the presence of proteins on as-synthesised IONPs, which may confer their stability. Preliminary investigation indicated the role of proteins in the synthesis and stabilization of IONPs. On the basis of present findings, a probable mechanism for synthesis of IONPs is suggested. The simplicity and versatility of the present approach can be utilized for the synthesis of other nanomaterials.

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Acknowledgments

This research was financially supported by the Indian Council of Agricultural Research, Government of India under the National Agricultural Innovation Project scheme (NAIP/C4/C-2032). Facilities provided by the Chonbuk National University, Jeonju, Republic of Korea, Advanced Instrumentation Research Facility, Jawaharlal Nehru University, New Delhi, India and Birla Institute of Technology and Science, Pilani, India are gratefully acknowledged. Arpit Bhargava and Navin Jain are thankful to the Indian Council of Agricultural Research and Council of Scientific and Industrial Research, Government of India, respectively, for providing research fellowship.

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Authors and Affiliations

  1. Centre for Biotechnology, Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, India

    Arpit Bhargava, Navin Jain, Manju Barathi L. & Jitendra Panwar

  2. Department of Applied Microbiology, College of Natural Sciences, Jimma University, Jimma, Ethiopia

    Mohd. Sayeed Akhtar

  3. Division of Environmental and Chemical Engineering, Chonbuk National University, Chonbuk, South Korea

    Yeoung-Sang Yun

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  1. Arpit Bhargava
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  2. Navin Jain
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  6. Jitendra Panwar
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Correspondence to Jitendra Panwar.

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Special Issue Editors: Mamadou Diallo, Neil Fromer, Myung S. Jhon

This article is part of the Topical Collection on Nanotechnology for Sustainable Development

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Bhargava, A., Jain, N., Barathi L., M. et al. Synthesis, characterization and mechanistic insights of mycogenic iron oxide nanoparticles. J Nanopart Res 15, 2031 (2013). https://doi.org/10.1007/s11051-013-2031-5

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