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A multigene phylogeny of theGibberella fujikuroi species complex: Detection of additional phylogenetically distinct species

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Mycoscience

Abstract

Phylogenetic relationships within theGibberella fujikuroi species complex were extended to newly discovered strains using nucleotide characters obtained by sequencing polymerase chain reaction (PCR) amplified DNA from 4 loci used in a previous study [nuclear large subunit 28S rDNA, nuclear ribosomal internal transcribed spacer (ITS) region, mitochondriaal small subunit (mtSSU) ribosomal DNA, and β-tubulin] together with two newly sampled protein-encoding nuclear genes, translation elongation factor EF-1α and calmodulin. Sequences from the ribosomal ITS region were analyzed separately and found to contain of two highly divergent, nonorthologous ITS2 types. Phylogenetic analysis of the individual and combined datasets identified 10 new phylogenetically distinct species distributed among the following three areas: 2 within Asia and 4 within both Africa and South America. Hypotheses of the monophyly ofFusarium subglutinans and its two formae speciales, f. sp.pini and f. sp.ananas, were strongly rejected by a likelihood analysis. Maximum parsimony results further indicate that the protein-encoding nuclear genes provide considerably more phylogenetic signal that the ribosomal genes sequenced. Relative apparent synapomorphy analysis was used to detect long-branch attraction taxa and to obtain a statistical measure of phylogenetic signal in the individual and combined datasets.

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Authors and Affiliations

  1. Microbial Properties Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Departement of Agriculture, 61604, Peoria, Illinois, USA

    Kerry O’Donnell & Elizabeth Cigelnik

  2. Biologische Bundesanstalt für Land-und Forstwirtschaft, Institut für Mikrobiologie, Konigin-Luise-Straße 19, D-14195, Berlin, Germany

    Helgard I. Nirenberg

  3. National Institute of Agrobiological Resources, Department of Genetic Resources I, Ministry of Agriculture, Forestry and Fisheries, Kannondai, 305-8602, Tsukuba, Ibaraki, Japan

    Takayuki Aoki

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  1. Kerry O’Donnell
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O’Donnell, K., Nirenberg, H.I., Aoki, T. et al. A multigene phylogeny of theGibberella fujikuroi species complex: Detection of additional phylogenetically distinct species. Mycoscience 41, 61–78 (2000). https://doi.org/10.1007/BF02464387

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