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Mycopathologia

, Volume 175, Issue 1–2, pp 159–164 | Cite as

Synergic Effect of Photodynamic Therapy with Methylene Blue and Surfactants in the Inhibition of Candida albicans

  • Juliana Pereira LyonEmail author
  • Rafael Reis Rezende
  • Mariana Penido Rabelo
  • Carlos José de Lima
  • Leonardo Marmo Moreira
Article

Abstract

Photodynamic therapy (PDT) has been originally developed for the treatment of cancer, but it has been successfully employed in the treatment of infectious diseases, including fungal infections. Surfactants are amphiphilic compounds that also have antifungal properties. The present work demonstrates the synergic effect of PDT with methylene blue (MB) and LED combined with four different surfactants in the killing of Candida albicans. Subinhibitory concentrations of CTAC, HPS, SDS and Triton X-100 were tested with MB PDT. The combined therapies proved to be more efficient than PDT or surfactants separately. The best results were obtained with CTAC and HPS and PDT with MB at the concentration of 32 μg/mL. In conclusion, the combination of surfactants and PDT is an alternative antifungal treatment that can achieve more effective performance with minimal discomfort to the patient.

Keywords

Photodynamic therapy Antifungal PDT Surfactants Candida albicans 

Notes

Acknowledgments

We thank to FAPEMIG and CNPq for the Scientific Initiation scholarships granted to Rafael Reis Resende and Mariana Penido Rabelo.

References

  1. 1.
    Dougherty TJ, Gomer CJ, Henderson BW, Jori G, Kessel D, Korbelik M, Moang J, Peng Q. Photodynamic therapy. J Natl Cancer Inst. 1998;90:889–905.PubMedCrossRefGoogle Scholar
  2. 2.
    Bliss JM, Bigelow CE, Foster TH, Haidaris CG. Susceptibility of Candida Species to photodynamic effects of photofrin. Antimicrob Agents Chemother. 2011;48:2000–6.CrossRefGoogle Scholar
  3. 3.
    Moreira LM, Santos FV, Lyon JP, Maftoum-Costa M, Pacheco-Soares C, Silva NS. Photodynamic therapy: porphyrins and phthalocyanines as photosensitizers. Aust J Chem. 2008;61:741–54.CrossRefGoogle Scholar
  4. 4.
    Bristow C, Hudson R, Paget TA, Boyle RW. Potential of cationic porphyrins for photodynamic treatment of cutaneous Leishmaniasis. Photodiag Photodyn Ther. 2006;3:162–7.CrossRefGoogle Scholar
  5. 5.
    Asilian A, Davami M. Comparison between the efficacy of photodynamic therapy and topical paromomycin in the treatment of old world cutaneous leishmaniasis: a placebo-controlled, randomized clinical trial. Clin Exp Dermatol. 2006;31:634–7.PubMedCrossRefGoogle Scholar
  6. 6.
    Silva NS, Ribeiro CM, Machado AH, Pacheco-Soares C. Ultrastructural changes in Tritrichomonas foetus after treatments with AlPcS4 and photodynamic therapy. Vet Parasitol. 2007;146:175–81.PubMedCrossRefGoogle Scholar
  7. 7.
    Wainwright M. Photodynamic antimicrobial chemotherapy. J Antimicrobiol. 1998;42:13–28.CrossRefGoogle Scholar
  8. 8.
    Lyon JP, Moreira LM, Moraes PCG, Santos FV, Resende MA. Photodynamic therapy for pathogenic fungi. Mycoses. 2011;54:E265–71.PubMedCrossRefGoogle Scholar
  9. 9.
    Lyon JP, Azevedo CMPS, Moreira LM, Lima CJ, Resende MA. Photodynamic antifungal therapy against chromoblastomycosis. Mycopathologia. 2011;172:293–7.PubMedCrossRefGoogle Scholar
  10. 10.
    Calzavara-Pinton PG, Venturin M, Sala R. A comprehensive overview of photodynamic therapy in the treatment of superficial fungal infections of the skin. J Photochem Photobiol B Biol. 2005;78:1–6.CrossRefGoogle Scholar
  11. 11.
    Munin E, Giroldo LM, Alves LP, Costa MS. Study of germ tube formation by Candida albicans after photodynamic antimicrobial chemotherapy (PACT). J Photochem Photobiol B Biol. 2007;88:16–20.CrossRefGoogle Scholar
  12. 12.
    Giroldo LM, Felipe MP, Oliveira MA, Munin E, Alves LP, Costa MS. Photodynamic antimicrobial chemotherapy (PACT) with methylene blue increases membrane permeability in Candida albicans. Lasers Med Sci. 2009;24:109–12.PubMedCrossRefGoogle Scholar
  13. 13.
    Chabrier-Roseló Y, Foster TH, Pérez-Nazario N, Mitra S, Haidaris CG. Sensitivity of Candida albicans germ tubes and biofilms to photofrin-mediated phototoxicity. Antimicrob Agents Chemother. 2005;49:4288–95.CrossRefGoogle Scholar
  14. 14.
    Dovigo LN, Pavarina AC, Ribeiro DG, Adriano CS, Bagnato VS. Photodynamic inactivation of four Candida species induced by photogem. Braz J Microbiol. 2010;41:42–9.CrossRefGoogle Scholar
  15. 15.
    Soares BM, Resende MA, Silva DL, Cisalpino PS. In vitro photodynamic inactivation of Candida spp. growth and adhesion to buccal epithelial cells. J Photochem Photobiol B Biol. 2008;94:65–70.CrossRefGoogle Scholar
  16. 16.
    Pinto PM, Oliveira RCBW, Lyon JP, Cury VF, Arantes RR, Koga-Ito CY, Resende MA. In vitro antifungal susceptibility of clinical isolates of Candida spp. obtained from patients with different predisposing factors to candidosis. Microbiol Res. 2008;63:579–85.CrossRefGoogle Scholar
  17. 17.
    Miksovska J, Yom J, Diamond B, Larsen RW. Spectroscopic and photothermal study of myoglobin conformational changes in the presence of sodium dodecyl sulfate. Biomacromolecules. 2006;7:476–82.PubMedCrossRefGoogle Scholar
  18. 18.
    Lyon JP, Santos FV, Moraes PCG, Moreira LM. Inhibition of virulence factors of Candida spp. by different surfactants. Mycopathologia. 2011;171:93–101.PubMedCrossRefGoogle Scholar
  19. 19.
    Ellepola AN, Samaranayake LP. Adhesion of oral Candida albicans to human buccal epithelial cells following limited exposure to antifungal agents. J Oral Pathol Med. 1998;27:225–32.Google Scholar
  20. 20.
    Lyon JP, Costa SC, Totti VMG, Munho MFV, Resende MA. Predisposing conditions for Candida spp. carriage in the oral cavity of denture wearers and individuals with natural teeth. Can J Microbiol. 2006;52:462–7.PubMedCrossRefGoogle Scholar
  21. 21.
    Lyon JP, Moreira LM, Cardoso MG, Saade J, Resende MA. Antifungal susceptibility profile of Candida spp. oral isolates obtained from healthy individuals. Braz J Microbiol. 2008;39:668–72.CrossRefGoogle Scholar
  22. 22.
    Dovigo LN, Pavarina AC, Oliveira M, Garcia E, Giampaolo ET, Vergani CE, Bagnato VS. Fungicidal effect of photodynamic therapy against fluconazole-resistant Candida albicans and Candida glabrata. Mycoses. 2009;54:123–30.CrossRefGoogle Scholar
  23. 23.
    Dovigo LN, Pavarina AC, Oliveira M, Garcia E, Giampaolo ET, Vergani CE, Bagnato VS. Fungicidal effect of photodynamic therapy against fluconazole-resistant Candida albicans and Candida glabrata. Mycoses. 2011;54:123–30.PubMedCrossRefGoogle Scholar
  24. 24.
    Teichert MC, Jones JW, Usacheva MN, Biel MA. Treatment of oral candidiasis with methylene blue-mediated photodynamic therapy in an immunodeficient murine model. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;93:155–60.PubMedCrossRefGoogle Scholar
  25. 25.
    Donnelly RF, McCarron PA, Tunney MM. Antifungal photodynamic therapy: a review. Microbiol Res. 2008;163:1–12.PubMedCrossRefGoogle Scholar
  26. 26.
    Moreira LM, Santiago PS, Almeida EV, Tabak M. Interaction of giant extracellular glossoscolex paulistus hemoglobin (HbGp) with zwitterionic surfactant N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (HPS): effects of oligomeric dissociation. Coll Surf B Biointerf. 2008;61:153–63.CrossRefGoogle Scholar
  27. 27.
    Liu W, Guo X, Guo R. The interaction between hemoglobin and two surfactants with different charges. Int J Biol Macromol. 2007;41:548–57.PubMedCrossRefGoogle Scholar
  28. 28.
    McCarron PA, Donnelly RF, Marouf W, Calvert DE. Anti-adherent and antifungal activities of surfactant-coated poly (ethylcyanoacrylate) nanoparticles. Int J Pharmacol. 2007;340:182–90.CrossRefGoogle Scholar
  29. 29.
    Wainwright M, Giddens RM. Phenotiazinium photosensitizers: choices in synthesis and applications. Dyes Pigment. 2003;57:245–57.CrossRefGoogle Scholar
  30. 30.
    Peloi LS, Biondo CEG, Kimura E, Politi MJ, Lonardoni MVC, Aristides SMA, Dorea RGC, Hioka N, Silveira TGV. Photodynamic therapy for American cutaneous leishmaniasis: the efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis. Exp Parasitol. 2011;128:353–6.PubMedCrossRefGoogle Scholar
  31. 31.
    Biyani N, Singh AK, Mandal S, Chawla B, Madhubala R. Differential expression of proteins in antimony-susceptible and—resistant isolates of Leishmania donovani. Mol Biochem Parasitol. 2011;179:91–9.PubMedCrossRefGoogle Scholar
  32. 32.
    Costa AC, de Campos Rasteiro VM, Pereira CA, da Silva Hashimoto ES, Beltrame M Jr, Junqueira JC, Jorge AO. Susceptibility of Candida albicans and Candida dubliniensis to erythrosine- and LED-mediated photodynamic therapy. Arch Oral Biol. 2011;56:1299–305.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Juliana Pereira Lyon
    • 1
    Email author
  • Rafael Reis Rezende
    • 1
  • Mariana Penido Rabelo
    • 1
  • Carlos José de Lima
    • 2
  • Leonardo Marmo Moreira
    • 3
  1. 1.Departamento de Ciências NaturaisUniversidade Federal de São João Del ReiSão João Del ReiBrazil
  2. 2.Universidade Camilo Castelo BrancoSão José dos CamposBrazil
  3. 3.Departamento de ZootecniaUniversidade Federal de São João Del ReiSão João Del ReiBrazil

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