Abstract
The incidence of candidemia and invasive candidiasis have increased markedly due to the increasing number of immunocompromised patients. There are five major medically important species of Candida with their frequency of isolation in the diminishing order namely Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata and Candida krusei. In addition, there are numerous other species of Candida which differ in their genetic makeup, virulence properties, drug susceptibilities and sugar assimilation capabilities. In this report, an unusual Candida species was isolated from the blood of two leukaemic patients. Conventional culture and biochemical tests identified the Candida species as C. parapsilosis. Using fungal-specific oligonucleotide primers ITS1 and ITS4, we managed to amplify the ribosomal RNA gene and its internal transcribed spacer region from the genomic DNA of these isolates. The PCR products were then purified and subjected to automated DNA sequencing using BLAST and CLUSTAL sequence analysis identified these isolates to be Candida orthopsilosis. Candida orthopsilosis is a new species recently identified in 2005, being morphologically indistinguishable from C. parapsilosis and was previously classified as a subspecies of C. parapsilosis. This report highlights the importance of complementing traditional culture and biochemical-based identification methods with DNA-based molecular assays such as PCR as the latter is more superior in terms of its discriminatory power and speed.
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Yang CW, Barkham TM, Chan FY, Wang Y. Prevalence of Candida species, including Candida dubliniensis, in Singapore. J Clin Microbiol. 2003;41:472–4.
Martin D, Persat F, Piens MA, Picot S. Candida species distribution in bloodstream cultures in Lyon, France, 1998–2001. Eur J Clin Microbiol Infect Dis. 2005;24:329–33.
Hajjeh RA, Sofair AN, Harrison LH, Lyon GM, Arthington-Skaggs BA, Mirza SA, Phelan M, Morgan J, Lee-Yang W, Ciblak MA, Benjamin LE, Sanza LT, Huie S, Yeo SF, Brandt ME, Warnock DW. Incidence of bloodstream infections due to Candida species and in vitro susceptibilities of isolates collected from 1998 to 2000 in a population-based active surveillance program. J Clin Microbiol. 2004;42:1519–27.
Ng KP, Saw TL, Na SL, Soo-Hoo TS. Systemic Candida infection in University hospital 1997–1999: the distribution of Candida biotypes and antifungal susceptibility patterns. Mycopathologia. 2001;149:141–6.
Ng KP, Madasamy M, Saw TL, Baki A, He J, Soo-Hoo TS. Candida biotypes isolated from clinical specimens in Malaysia. Mycopathologia. 1999;144:135–40.
Rycovska A, Valach M, Tomaska L, Bolotin-Fukuhara M, Nosek J. Linear versus circular mitochondrial genomes: intraspecies variability of mitochondrial genome architecture in Candida parapsilosis. Microbiology. 2004;150:1571–80.
Tavanti A, Davidson AD, Gow NA, Maiden MC, Odds FC. Candida orthopsilosis and Candida metapsilosis spp. nov. to replace Candida parapsilosis groups II and III. J Clin Microbiol. 2005;43:284–92.
Scherer S, Stevens DA. Application of DNA typing methods to epidemiology and taxonomy of Candida species. J Clin Microbiol. 1987;25:675–9.
Lehmann PF, Lin DM, Lasker BA. Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA. J Clin Microbiol. 1992;30:3249–54.
Lin DM, Wu LC, Rinaldi MG, Lehmann PF. Three distinct genotypes within Candida parapsilosis from clinical sources. J Clin Microbiol. 1995;33:1815–21.
Levin AS, Costa SF, Mussi NS, Basso M, Sinto SI, Machado C, Geiger DC, Villares MC, Schreiber AZ, Barone AA, Branchini ML. Candida parapsilosis fungemia associated with implantable and semi-implantable central venous catheters and the hands of healthcare workers. Diagn Microbiol Infect Dis. 1998;30:243–9.
Ellepola AN, Hurst BSF, Elie CM, Morrison CJ. Rapid and unequivocal differentiation of Candida dubliniensis from other Candida species using species-specific DNA probes: comparison with phenotypic identification methods. Oral Microbiol Immunol. 2003;18:379–88.
Fisher MC, Koenig GL, White TJ, Taylor JT. Molecular and phenotypic description of Coccidioides posadasii sp. nov., previously recognized as the non-California population of Coccidioides immitis. Mycologia 2002;94:73–84.
Pfaller MA, Jones RN, Doern GV, Fluit AC, Verhoef J, Sader HS, Messer SA, Houston A, Coffman S, Hollis RJ, et al. International surveillance of blood stream infections due to Candida species in the European SENTRY program: species distribution and antifungal susceptibility including the investigational triazole and echinocandin agents. Diagn Microbiol Infect Dis. 1999;35:19–25.
Pagano L, Antinori A, Ammassari A, Mele L, Nosari A, Melillo L, Martino B, Sanguinetti M, Equitani F, Nobile F, Carotenuto M, Morra E, Morace G, Leone G. Retrospective study of candidemia in patients with hematological malignancies: clinical features, risk factors, and outcome of 76 episodes. Eur J Hematol. 1999;63:77–85.
Krcmery V Jr, Kovacicova G, et al. Longitudinal 10-year prospective survey of fungaemia in Slovak Republic: trends in etiology in 310 episodes. Diagn Microbiol Infect Dis. 2000;36:7–11.
Lupetti A, Tavanti A, Davini P, Ghelardi E, Corsini V, Merusi I, Boldrini A, Campa M, Senesi S. Horizontal transmission of Candida parapsilosis candidemia in a neonatal intensive care unit. J Clin Microbiol. 2002;40:2363–9.
Pfaller MA, Diekema DJ, Jones RN, Messer SA, Hollis RJ. Trends in antifungal susceptibility of Candida spp. isolated from pediatric and adult patients with bloodstream infections: SENTRY antimicrobial surveillance program, 1997–2000. J Clin Microbiol. 2002;40:852–6.
Saiman L, Ludington E, Dawson JD, Patterson JE, Rangel-Frausto S, Wiblin RT, Blumberg HM, Pfaller M, Rinaldi M, Edwards JE, Wenzel RP, Jarvis W. Risk factors for Candida species colonization of neonatal intensive care unit patients. Pediatr Infect Dis J. 2001;20:1119–24.
Kuhn DM, Mikherjee PK, Clark TA, Pujol C, Chandra J, Hajjeh RA, Warnock DW, Soil DR, Ghannoum MA. Candida parapsilosis characterization in an outbreak setting. Emerg Infect Dis. 2004;10:1074–81.
Sanchez V, Vazquez JA, Barth-Jones D, Dembry L, Sobel JD, Zervos MJ. Nosocomial acquisition of Candida parapsilosis: an epidemiologic study. Am J Med. 1993;94:577–82.
Song JW, Shin JH, Shint DH, Jung SI, Cho D, Kee SJ, Shin MG, Suh SP, Ryang DW. Differences in biofilm production by three genotypes of Candida parapsilosis from clinical sources. Med Mycol. 2005;43:657–61.
Shin JH, Kee SJ, Shin MG, Kim SH, Shin DH, Lee SK, Suh SP, Ryang DW. Biofilm production by isolates of Candida species recovered from nonneutropenic patients: comparison of bloodstream isolates with isolates from other sources. J Clin Microbiol. 2002;40:1244–8.
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The authors are grateful to University Putra Malaysia for the Research University Grant Scheme (RUGS) which provided the financial support for this project.
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Yong, P.V.C., Chong, P.P., Lau, L.Y. et al. Molecular Identification of Candida orthopsilosis Isolated from Blood Culture . Mycopathologia 165, 81–87 (2008). https://doi.org/10.1007/s11046-007-9086-8
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DOI: https://doi.org/10.1007/s11046-007-9086-8