Skip to main content

The mycological and molecular study of Hortaea werneckii isolated from blood and splenic abscess

Mycopathologia volume 159, pages 495–500 (2005)Cite this article

Abstract

Hortaea werneckii is an environmental dematiaceous fungus found in the halophilic environment. It causes tinea nigra. We report the isolation of H. werneckii from blood and splenic abscess of two patients with acute myelomonocytic leukaemia. H. werneckii grew at room temperature but not at 37 °C, it was identified by biochemical tests, growth characteristics and the presence of conspicuous collarette intercalary on dividing yeast cells. The use of specific oligonucleotide primer Hor-F (5′-TGGACACCTTCA TAACTCTTG-3′) and Hor-R (5′-TCACAACGCTTAGAGACGG-3′) confirmed the two isolates were H. werneckii. The sequence for 281 nucleotide of HW299 and HW403 were 99% identical but differed only in one nucleotide. In vitro anti-fungal susceptibility testing showed that the isolates were resistant to amphotericin B and flucytosine.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

References

  1. MB Edmond SE Wallace DK McClish MA Pfaller RN Jones RP Wenzel (1999) ArticleTitleNosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin Infect Dis 29 239–244 Occurrence Handle1:STN:280:DyaK1MvgsVeluw%3D%3D Occurrence Handle10476719

    CAS  PubMed  Google Scholar 

  2. GS Hoog Particlede J Guarro J Gene MJ Figueras (2000) Atlas of clinical fungi EditionNumber2 Centraalbureau voor Schimmelcultures Utrecht Netherlands

    Google Scholar 

  3. P Abliz K Fukushima K Takizawa M Miyaji K Nishimura (2003) ArticleTitleOligonucleotide primrs for identification of Hortaea werneckii, a causative agent of tinea nigra. Diagn Microbiol Infect Dis 46 89–93 Occurrence Handle10.1016/S0732-8893(03)00035-X Occurrence Handle1:CAS:528:DC%2BD3sXksF2nt7w%3D Occurrence Handle12812723

    Article  CAS  PubMed  Google Scholar 

  4. S Sharmin K Haritani R Tanaka P Abliz K takizawa A Sano K Fukushima K Nishimura M Miyagi (2002) ArticleTitleThe first isolation of Hortaea werneckii from a household guinea pig. Nippon Ishinkin Gakki Zasshi 43 IssueID3 175–180 Occurrence Handle1:CAS:528:DC%2BD38Xns1yrtbw%3D

    CAS  Google Scholar 

  5. CE Huber T La Berge T Schwiesow K Carroll PS Bernstain N Mamalis (2000) ArticleTitleExophiala werneckii endophthalmitis following cataract surgery in an immunocompetent individual. Ophthal Surg Lasers. 31 IssueID5 417–422 Occurrence Handle1:STN:280:DC%2BD3cvltlClsg%3D%3D

    CAS  Google Scholar 

  6. S Shadomy H Wang HJ Shadomy (1988) ArticleTitleFurther in-vitro studies with oxiconazole nitrate. Diagn Microbiol Infect Dis 9 231–237 Occurrence Handle10.1016/0732-8893(88)90114-9 Occurrence Handle1:CAS:528:DyaL1cXlvVOisrY%3D Occurrence Handle3180708

    Article  CAS  PubMed  Google Scholar 

  7. R Sayegh-Carreno W Abramovits-Ackerman GM Giron (1989) ArticleTitleTherapy of tinea plantaris. Int J Dermatol 28 46–48 Occurrence Handle1:STN:280:BiaC3sfns1E%3D Occurrence Handle2917812

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

    K. P. Ng, T. S. Soo-Hoo, S. L. Na, S. T. Tay & H. Hamimah

  2. Department of Biomedical Sciences, Faculty of Medicine and Health Science, University Putra Malaysia, Serdang, Selangor, Malaysia

    P. C. Lim, P. P. Chong & H. F. Seow

  3. Department of Pathology, Roy J & Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa , 52242, USA

    A. J. Chavez & S. A. Messer

Authors
  1. K. P. Ng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. S. Soo-Hoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. L. Na
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. T. Tay
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Hamimah
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. C. Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. P. P. Chong
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. H. F. Seow
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. J. Chavez
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. S. A. Messer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. P. Ng.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ng, K.P., Soo-Hoo, T.S., Na, S.L. et al. The mycological and molecular study of Hortaea werneckii isolated from blood and splenic abscess. Mycopathologia 159, 495–500 (2005). https://doi.org/10.1007/s11046-005-1154-3

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11046-005-1154-3

Key words

  • acute myeloid leukaemia
  • dematiaceous fungi
  • Hortaea werneckii
  • oligonucleotide primer
  • tinea nigra