Hortaea werneckii is an environmental dematiaceous fungus found in the halophilic environment. It causes tinea nigra. We report the isolation of H. werneckii from blood and splenic abscess of two patients with acute myelomonocytic leukaemia. H. werneckii grew at room temperature but not at 37 °C, it was identified by biochemical tests, growth characteristics and the presence of conspicuous collarette intercalary on dividing yeast cells. The use of specific oligonucleotide primer Hor-F (5′-TGGACACCTTCA TAACTCTTG-3′) and Hor-R (5′-TCACAACGCTTAGAGACGG-3′) confirmed the two isolates were H. werneckii. The sequence for 281 nucleotide of HW299 and HW403 were 99% identical but differed only in one nucleotide. In vitro anti-fungal susceptibility testing showed that the isolates were resistant to amphotericin B and flucytosine.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
MB Edmond SE Wallace DK McClish MA Pfaller RN Jones RP Wenzel (1999) ArticleTitleNosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin Infect Dis 29 239–244 Occurrence Handle1:STN:280:DyaK1MvgsVeluw%3D%3D Occurrence Handle10476719
GS Hoog Particlede J Guarro J Gene MJ Figueras (2000) Atlas of clinical fungi EditionNumber2 Centraalbureau voor Schimmelcultures Utrecht Netherlands
P Abliz K Fukushima K Takizawa M Miyaji K Nishimura (2003) ArticleTitleOligonucleotide primrs for identification of Hortaea werneckii, a causative agent of tinea nigra. Diagn Microbiol Infect Dis 46 89–93 Occurrence Handle10.1016/S0732-8893(03)00035-X Occurrence Handle1:CAS:528:DC%2BD3sXksF2nt7w%3D Occurrence Handle12812723
S Sharmin K Haritani R Tanaka P Abliz K takizawa A Sano K Fukushima K Nishimura M Miyagi (2002) ArticleTitleThe first isolation of Hortaea werneckii from a household guinea pig. Nippon Ishinkin Gakki Zasshi 43 IssueID3 175–180 Occurrence Handle1:CAS:528:DC%2BD38Xns1yrtbw%3D
CE Huber T La Berge T Schwiesow K Carroll PS Bernstain N Mamalis (2000) ArticleTitleExophiala werneckii endophthalmitis following cataract surgery in an immunocompetent individual. Ophthal Surg Lasers. 31 IssueID5 417–422 Occurrence Handle1:STN:280:DC%2BD3cvltlClsg%3D%3D
S Shadomy H Wang HJ Shadomy (1988) ArticleTitleFurther in-vitro studies with oxiconazole nitrate. Diagn Microbiol Infect Dis 9 231–237 Occurrence Handle10.1016/0732-8893(88)90114-9 Occurrence Handle1:CAS:528:DyaL1cXlvVOisrY%3D Occurrence Handle3180708
R Sayegh-Carreno W Abramovits-Ackerman GM Giron (1989) ArticleTitleTherapy of tinea plantaris. Int J Dermatol 28 46–48 Occurrence Handle1:STN:280:BiaC3sfns1E%3D Occurrence Handle2917812
Rights and permissions
About this article
Cite this article
Ng, K.P., Soo-Hoo, T.S., Na, S.L. et al. The mycological and molecular study of Hortaea werneckii isolated from blood and splenic abscess. Mycopathologia 159, 495–500 (2005). https://doi.org/10.1007/s11046-005-1154-3
- acute myeloid leukaemia
- dematiaceous fungi
- Hortaea werneckii
- oligonucleotide primer
- tinea nigra