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Overexpression of Zostera japonica J protein gene ZjDjB1 in Arabidopsis enhanced the tolerance to lead stress

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Abstract

Background

Among the heavy metal pollution in soil, lead pollution is particularly prominent. The lead in contaminated soil will not only cause damage to plants, animals and microorganisms, but also seriously affect the progress of the entire ecosystem. Under lead stress, the abundance of DnaJ protein in plants will increase. However, little is known about the role of DnaJ in lead stress.

Methods and results

We used transgenic Arabidopsis that overexpressed DnaJ gene ZjDjB1 of Zostera japonica as material to study the role of DnaJ in the mechanism of lead induced stress response. Under lead stress, the seedlings and adult plants of transgenic ZjDjB1 Arabidopsis have higher tolerance to lead stress than wild type. Under lead stress, the content of NO and O2·− free radicals in transgenic ZjDjB1 Arabidopsis was lower than that of wild type. The negative effect of catalase in transgenic ZjDjB1 Arabidopsis under lead stress was weaker than that of wild type. The expression of ABC transporter of mitochondrion 3 (ATM3; systematic name: ABCB25) in transgenic ZjDjB1 Arabidopsis under lead stress was higher than that in wild type.

Conclusions

These results confirmed that ZjDjB1, the DnaJ gene of Z. japonica, was involved in the reaction mechanism to lead pollution, which might improve the tolerance of plants to lead stress by maintaining catalase activity and increasing the expression level of ATM3 under lead stress.

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Data availability

All data generated or analyzed during this study are included in this published article or supplementary file. The nucleotide and deduced amino acid sequence data of ZjDjB1 were registered in GenBank (No. MN395290).

Code availability

Not applicable.

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Funding

This study was funded by the Natural Science Foundation of Guangxi Province (2020GXNSFAA297067), the Research Fund Program of Guangxi Key Lab of Mangrove Conservation and Utilization (GKLMC-22A02; GKLMC-21A01; GKLMC-20A04; GKLMC-20A01), the National Natural Science Foundation of China (32170399) and the National Science & Technology Fundamental Resources Investigation Program of China (2019FY100604). These funding bodies had no role in the design of the study, collection, analysis, and interpretation of data, or in the writing of the manuscript.

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Authors and Affiliations

  1. Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Academy of Marine Sciences (Guangxi Mangrove Research Center), Guangxi Academy of Sciences, Beihai, 536007, Guangxi, China

    Siting Chen & Guanglong Qiu

Authors
  1. Siting Chen
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  2. Guanglong Qiu
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Contributions

SC designed and conducted the experiment. GQ conducted field sampling and identification. SC and GQ wrote the manuscript. All the authors reviewed the manuscript.

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Correspondence to Siting Chen or Guanglong Qiu.

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Chen, S., Qiu, G. Overexpression of Zostera japonica J protein gene ZjDjB1 in Arabidopsis enhanced the tolerance to lead stress. Mol Biol Rep 50, 5117–5124 (2023). https://doi.org/10.1007/s11033-023-08470-w

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  • DOI: https://doi.org/10.1007/s11033-023-08470-w

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